Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS18745 and BPHYT_RS18750 are separated by 3 nucleotides BPHYT_RS18750 and BPHYT_RS18755 overlap by 1 nucleotides BPHYT_RS18755 and BPHYT_RS18760 are separated by 91 nucleotides BPHYT_RS18760 and BPHYT_RS18765 overlap by 4 nucleotides
BPHYT_RS18745: BPHYT_RS18745 - coniferyl aldehyde dehydrogenase, at 4,216,245 to 4,217,663
_RS18745
BPHYT_RS18750: BPHYT_RS18750 - flagellar biosynthetic protein FlhB, at 4,217,667 to 4,218,026
_RS18750
BPHYT_RS18755: BPHYT_RS18755 - flagellar hook-length control protein FliK, at 4,218,026 to 4,219,459
_RS18755
BPHYT_RS18760: BPHYT_RS18760 - flagellar biosynthesis protein FliT, at 4,219,551 to 4,219,877
_RS18760
BPHYT_RS18765: BPHYT_RS18765 - flagellar biosynthesis protein FliS, at 4,219,874 to 4,220,308
_RS18765
Position (kb) 4218
4219
4220 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 4217.083 kb on - strand, within BPHYT_RS18745 at 4217.319 kb on + strand, within BPHYT_RS18745 at 4217.319 kb on + strand, within BPHYT_RS18745 at 4217.319 kb on + strand, within BPHYT_RS18745 at 4217.320 kb on - strand, within BPHYT_RS18745 at 4217.379 kb on + strand, within BPHYT_RS18745 at 4217.379 kb on + strand, within BPHYT_RS18745 at 4217.380 kb on - strand, within BPHYT_RS18745 at 4217.385 kb on + strand, within BPHYT_RS18745 at 4217.523 kb on + strand at 4217.524 kb on - strand at 4217.813 kb on - strand, within BPHYT_RS18750 at 4217.813 kb on - strand, within BPHYT_RS18750 at 4217.887 kb on + strand, within BPHYT_RS18750 at 4217.887 kb on + strand, within BPHYT_RS18750 at 4217.887 kb on + strand, within BPHYT_RS18750 at 4217.939 kb on - strand, within BPHYT_RS18750 at 4217.987 kb on - strand, within BPHYT_RS18750 at 4217.987 kb on - strand, within BPHYT_RS18750 at 4218.157 kb on - strand at 4219.397 kb on - strand at 4219.498 kb on + strand at 4219.498 kb on + strand at 4219.499 kb on - strand at 4219.499 kb on - strand at 4219.520 kb on + strand at 4219.520 kb on + strand at 4219.520 kb on + strand at 4219.685 kb on - strand, within BPHYT_RS18760 at 4219.685 kb on - strand, within BPHYT_RS18760 at 4219.759 kb on + strand, within BPHYT_RS18760 at 4220.030 kb on - strand, within BPHYT_RS18765 at 4220.030 kb on - strand, within BPHYT_RS18765 at 4220.030 kb on - strand, within BPHYT_RS18765 at 4220.030 kb on - strand, within BPHYT_RS18765 at 4220.030 kb on - strand, within BPHYT_RS18765 at 4220.032 kb on - strand, within BPHYT_RS18765 at 4220.032 kb on - strand, within BPHYT_RS18765 at 4220.032 kb on - strand, within BPHYT_RS18765 at 4220.036 kb on - strand, within BPHYT_RS18765 at 4220.036 kb on - strand, within BPHYT_RS18765 at 4220.038 kb on - strand, within BPHYT_RS18765 at 4220.038 kb on - strand, within BPHYT_RS18765 at 4220.275 kb on - strand at 4220.275 kb on - strand at 4220.388 kb on - strand at 4220.388 kb on - strand at 4220.388 kb on - strand at 4220.388 kb on - strand at 4220.420 kb on + strand at 4220.421 kb on - strand at 4220.440 kb on + strand at 4220.441 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 4,217,083 - BPHYT_RS18745 0.59 +0.9 4,217,319 + BPHYT_RS18745 0.76 +1.0 4,217,319 + BPHYT_RS18745 0.76 +0.0 4,217,319 + BPHYT_RS18745 0.76 +0.9 4,217,320 - BPHYT_RS18745 0.76 +0.0 4,217,379 + BPHYT_RS18745 0.80 +1.3 4,217,379 + BPHYT_RS18745 0.80 -0.8 4,217,380 - BPHYT_RS18745 0.80 -1.3 4,217,385 + BPHYT_RS18745 0.80 +0.0 4,217,523 + +0.1 4,217,524 - -1.8 4,217,813 - BPHYT_RS18750 0.41 +0.1 4,217,813 - BPHYT_RS18750 0.41 +0.7 4,217,887 + BPHYT_RS18750 0.61 +1.5 4,217,887 + BPHYT_RS18750 0.61 +0.3 4,217,887 + BPHYT_RS18750 0.61 -0.6 4,217,939 - BPHYT_RS18750 0.76 +1.4 4,217,987 - BPHYT_RS18750 0.89 +1.2 4,217,987 - BPHYT_RS18750 0.89 +1.6 4,218,157 - +0.3 4,219,397 - +0.0 4,219,498 + +0.0 4,219,498 + +0.7 4,219,499 - -0.1 4,219,499 - +0.9 4,219,520 + +0.0 4,219,520 + +1.4 4,219,520 + +0.2 4,219,685 - BPHYT_RS18760 0.41 -1.7 4,219,685 - BPHYT_RS18760 0.41 +1.5 4,219,759 + BPHYT_RS18760 0.64 +0.3 4,220,030 - BPHYT_RS18765 0.36 -3.4 4,220,030 - BPHYT_RS18765 0.36 -2.5 4,220,030 - BPHYT_RS18765 0.36 -0.1 4,220,030 - BPHYT_RS18765 0.36 -2.7 4,220,030 - BPHYT_RS18765 0.36 -3.5 4,220,032 - BPHYT_RS18765 0.36 -1.2 4,220,032 - BPHYT_RS18765 0.36 -4.5 4,220,032 - BPHYT_RS18765 0.36 -1.8 4,220,036 - BPHYT_RS18765 0.37 -2.6 4,220,036 - BPHYT_RS18765 0.37 -3.3 4,220,038 - BPHYT_RS18765 0.38 -1.0 4,220,038 - BPHYT_RS18765 0.38 -1.6 4,220,275 - -1.3 4,220,275 - -3.1 4,220,388 - +1.0 4,220,388 - -0.1 4,220,388 - -0.7 4,220,388 - -0.3 4,220,420 + +0.7 4,220,421 - +0.9 4,220,440 + +1.3 4,220,441 - +0.3
Or see this region's nucleotide sequence