Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS13640

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS13625 and BPHYT_RS13630 are separated by 158 nucleotidesBPHYT_RS13630 and BPHYT_RS13635 are separated by 29 nucleotidesBPHYT_RS13635 and BPHYT_RS13640 are separated by 246 nucleotidesBPHYT_RS13640 and BPHYT_RS13645 are separated by 103 nucleotides BPHYT_RS13625: BPHYT_RS13625 - phenylalanine--tRNA ligase alpha subunit, at 3,106,841 to 3,107,854 _RS13625 BPHYT_RS13630: BPHYT_RS13630 - 50S ribosomal protein L20, at 3,108,013 to 3,108,372 _RS13630 BPHYT_RS13635: BPHYT_RS13635 - 50S ribosomal protein L35, at 3,108,402 to 3,108,599 _RS13635 BPHYT_RS13640: BPHYT_RS13640 - translation initiation factor IF-3, at 3,108,846 to 3,109,316 _RS13640 BPHYT_RS13645: BPHYT_RS13645 - threonine--tRNA ligase, at 3,109,420 to 3,111,327 _RS13645 Position (kb) 3108 3109 3110Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 3108.735 kb on - strandat 3108.735 kb on - strandat 3108.735 kb on - strandat 3109.557 kb on - strandat 3109.591 kb on - strandat 3109.592 kb on + strandat 3109.593 kb on - strandat 3109.593 kb on - strandat 3109.593 kb on - strandat 3109.597 kb on - strandat 3109.597 kb on - strandat 3109.597 kb on - strandat 3109.597 kb on - strandat 3109.597 kb on - strandat 3109.597 kb on - strandat 3109.597 kb on - strandat 3109.625 kb on - strand, within BPHYT_RS13645at 3109.671 kb on - strand, within BPHYT_RS13645at 3109.872 kb on + strand, within BPHYT_RS13645at 3109.928 kb on + strand, within BPHYT_RS13645at 3109.929 kb on - strand, within BPHYT_RS13645at 3109.929 kb on - strand, within BPHYT_RS13645at 3109.929 kb on - strand, within BPHYT_RS13645at 3109.946 kb on + strand, within BPHYT_RS13645at 3110.108 kb on + strand, within BPHYT_RS13645at 3110.108 kb on + strand, within BPHYT_RS13645at 3110.108 kb on + strand, within BPHYT_RS13645at 3110.109 kb on - strand, within BPHYT_RS13645at 3110.109 kb on - strand, within BPHYT_RS13645at 3110.109 kb on - strand, within BPHYT_RS13645at 3110.128 kb on - strand, within BPHYT_RS13645at 3110.128 kb on - strand, within BPHYT_RS13645at 3110.176 kb on - strand, within BPHYT_RS13645at 3110.176 kb on - strand, within BPHYT_RS13645at 3110.242 kb on - strand, within BPHYT_RS13645at 3110.268 kb on - strand, within BPHYT_RS13645at 3110.268 kb on - strand, within BPHYT_RS13645

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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3,108,735 - -0.8
3,108,735 - -2.2
3,108,735 - -2.8
3,109,557 - +1.2
3,109,591 - +1.7
3,109,592 + -0.7
3,109,593 - +3.0
3,109,593 - +3.8
3,109,593 - +1.1
3,109,597 - +1.2
3,109,597 - +0.9
3,109,597 - +1.1
3,109,597 - +1.3
3,109,597 - +0.0
3,109,597 - +2.1
3,109,597 - +3.1
3,109,625 - BPHYT_RS13645 0.11 +1.2
3,109,671 - BPHYT_RS13645 0.13 +2.0
3,109,872 + BPHYT_RS13645 0.24 +3.8
3,109,928 + BPHYT_RS13645 0.27 +1.6
3,109,929 - BPHYT_RS13645 0.27 +1.6
3,109,929 - BPHYT_RS13645 0.27 +2.3
3,109,929 - BPHYT_RS13645 0.27 +2.8
3,109,946 + BPHYT_RS13645 0.28 +4.0
3,110,108 + BPHYT_RS13645 0.36 +2.8
3,110,108 + BPHYT_RS13645 0.36 +2.9
3,110,108 + BPHYT_RS13645 0.36 +2.5
3,110,109 - BPHYT_RS13645 0.36 +1.9
3,110,109 - BPHYT_RS13645 0.36 +2.7
3,110,109 - BPHYT_RS13645 0.36 +2.6
3,110,128 - BPHYT_RS13645 0.37 +3.1
3,110,128 - BPHYT_RS13645 0.37 +1.7
3,110,176 - BPHYT_RS13645 0.40 +1.4
3,110,176 - BPHYT_RS13645 0.40 +2.6
3,110,242 - BPHYT_RS13645 0.43 +2.5
3,110,268 - BPHYT_RS13645 0.44 +2.6
3,110,268 - BPHYT_RS13645 0.44 +3.8

Or see this region's nucleotide sequence