Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS08850

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS08840 and BPHYT_RS08845 are separated by 145 nucleotidesBPHYT_RS08845 and BPHYT_RS08850 overlap by 11 nucleotidesBPHYT_RS08850 and BPHYT_RS08855 are separated by 293 nucleotidesBPHYT_RS08855 and BPHYT_RS08860 are separated by 51 nucleotides BPHYT_RS08840: BPHYT_RS08840 - LysR family transcriptional regulator, at 2,003,850 to 2,004,764 _RS08840 BPHYT_RS08845: BPHYT_RS08845 - RNA polymerase sigma factor, at 2,004,910 to 2,005,644 _RS08845 BPHYT_RS08850: BPHYT_RS08850 - transcriptional regulator, at 2,005,634 to 2,006,569 _RS08850 BPHYT_RS08855: BPHYT_RS08855 - 30S ribosomal protein S6, at 2,006,863 to 2,007,237 _RS08855 BPHYT_RS08860: BPHYT_RS08860 - primosomal replication protein N, at 2,007,289 to 2,007,588 _RS08860 Position (kb) 2005 2006 2007Strain fitness (log2 ratio) -3 -2 -1 0 1at 2004.646 kb on - strand, within BPHYT_RS08840at 2004.700 kb on - strandat 2004.700 kb on - strandat 2004.726 kb on + strandat 2005.013 kb on - strand, within BPHYT_RS08845at 2005.159 kb on + strand, within BPHYT_RS08845at 2005.642 kb on + strandat 2006.145 kb on + strand, within BPHYT_RS08850at 2006.307 kb on - strand, within BPHYT_RS08850at 2006.369 kb on + strand, within BPHYT_RS08850at 2006.370 kb on - strand, within BPHYT_RS08850at 2006.624 kb on + strandat 2006.625 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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2,004,646 - BPHYT_RS08840 0.87 +1.2
2,004,700 - +1.2
2,004,700 - -0.7
2,004,726 + +0.1
2,005,013 - BPHYT_RS08845 0.14 +0.1
2,005,159 + BPHYT_RS08845 0.34 -0.0
2,005,642 + -1.0
2,006,145 + BPHYT_RS08850 0.55 -2.8
2,006,307 - BPHYT_RS08850 0.72 -3.5
2,006,369 + BPHYT_RS08850 0.79 -2.7
2,006,370 - BPHYT_RS08850 0.79 -2.2
2,006,624 + +0.3
2,006,625 - +1.3

Or see this region's nucleotide sequence