Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS06535

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS06525 and BPHYT_RS06530 are separated by 16 nucleotidesBPHYT_RS06530 and BPHYT_RS06535 are separated by 16 nucleotidesBPHYT_RS06535 and BPHYT_RS06540 overlap by 4 nucleotidesBPHYT_RS06540 and BPHYT_RS06545 are separated by 361 nucleotides BPHYT_RS06525: BPHYT_RS06525 - membrane protein, at 1,482,741 to 1,483,265 _RS06525 BPHYT_RS06530: BPHYT_RS06530 - membrane protein, at 1,483,282 to 1,484,040 _RS06530 BPHYT_RS06535: BPHYT_RS06535 - membrane protein, at 1,484,057 to 1,484,485 _RS06535 BPHYT_RS06540: BPHYT_RS06540 - RNA polymerase sigma70 factor, at 1,484,482 to 1,485,045 _RS06540 BPHYT_RS06545: BPHYT_RS06545 - acetolactate synthase, at 1,485,407 to 1,487,170 _RS06545 Position (kb) 1484 1485Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 1483.133 kb on - strand, within BPHYT_RS06525at 1483.174 kb on + strand, within BPHYT_RS06525at 1483.175 kb on - strand, within BPHYT_RS06525at 1483.210 kb on - strand, within BPHYT_RS06525at 1483.575 kb on - strand, within BPHYT_RS06530at 1483.629 kb on - strand, within BPHYT_RS06530at 1483.967 kb on + strandat 1484.034 kb on - strandat 1484.077 kb on - strandat 1484.101 kb on - strand, within BPHYT_RS06535at 1484.126 kb on + strand, within BPHYT_RS06535at 1484.127 kb on - strand, within BPHYT_RS06535at 1484.127 kb on - strand, within BPHYT_RS06535at 1484.181 kb on + strand, within BPHYT_RS06535at 1484.181 kb on + strand, within BPHYT_RS06535at 1484.485 kb on + strandat 1484.485 kb on + strandat 1484.486 kb on - strandat 1484.601 kb on + strand, within BPHYT_RS06540at 1484.602 kb on - strand, within BPHYT_RS06540at 1484.602 kb on - strand, within BPHYT_RS06540at 1484.811 kb on + strand, within BPHYT_RS06540at 1484.811 kb on + strand, within BPHYT_RS06540at 1484.811 kb on + strand, within BPHYT_RS06540at 1484.811 kb on + strand, within BPHYT_RS06540at 1484.838 kb on - strand, within BPHYT_RS06540at 1484.862 kb on + strand, within BPHYT_RS06540at 1484.862 kb on + strand, within BPHYT_RS06540at 1484.863 kb on - strand, within BPHYT_RS06540at 1484.863 kb on - strand, within BPHYT_RS06540at 1484.975 kb on + strand, within BPHYT_RS06540at 1485.077 kb on + strandat 1485.113 kb on + strandat 1485.162 kb on + strandat 1485.163 kb on - strandat 1485.163 kb on - strandat 1485.224 kb on - strandat 1485.224 kb on - strandat 1485.253 kb on + strandat 1485.274 kb on + strandat 1485.274 kb on + strandat 1485.275 kb on - strandat 1485.275 kb on - strandat 1485.412 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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1,483,133 - BPHYT_RS06525 0.75 -0.1
1,483,174 + BPHYT_RS06525 0.82 +0.1
1,483,175 - BPHYT_RS06525 0.83 -0.1
1,483,210 - BPHYT_RS06525 0.89 -1.7
1,483,575 - BPHYT_RS06530 0.39 -4.8
1,483,629 - BPHYT_RS06530 0.46 -1.7
1,483,967 + -3.2
1,484,034 - -4.0
1,484,077 - -2.5
1,484,101 - BPHYT_RS06535 0.10 -3.3
1,484,126 + BPHYT_RS06535 0.16 -3.8
1,484,127 - BPHYT_RS06535 0.16 -4.7
1,484,127 - BPHYT_RS06535 0.16 -1.7
1,484,181 + BPHYT_RS06535 0.29 +0.1
1,484,181 + BPHYT_RS06535 0.29 -0.1
1,484,485 + +0.5
1,484,485 + -1.8
1,484,486 - -4.8
1,484,601 + BPHYT_RS06540 0.21 +0.0
1,484,602 - BPHYT_RS06540 0.21 +0.6
1,484,602 - BPHYT_RS06540 0.21 +0.4
1,484,811 + BPHYT_RS06540 0.58 +0.6
1,484,811 + BPHYT_RS06540 0.58 +0.8
1,484,811 + BPHYT_RS06540 0.58 -0.1
1,484,811 + BPHYT_RS06540 0.58 -0.9
1,484,838 - BPHYT_RS06540 0.63 +0.1
1,484,862 + BPHYT_RS06540 0.67 +0.3
1,484,862 + BPHYT_RS06540 0.67 -1.8
1,484,863 - BPHYT_RS06540 0.68 -0.5
1,484,863 - BPHYT_RS06540 0.68 +0.3
1,484,975 + BPHYT_RS06540 0.87 +0.1
1,485,077 + +0.2
1,485,113 + +0.6
1,485,162 + +0.2
1,485,163 - +0.5
1,485,163 - -0.4
1,485,224 - +0.1
1,485,224 - -0.2
1,485,253 + -0.5
1,485,274 + -0.1
1,485,274 + +1.1
1,485,275 - -0.0
1,485,275 - -0.5
1,485,412 + +1.3

Or see this region's nucleotide sequence