Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS04450

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS04445 and BPHYT_RS04450 are separated by 224 nucleotidesBPHYT_RS04450 and BPHYT_RS04455 are separated by 12 nucleotides BPHYT_RS04445: BPHYT_RS04445 - amino acid ABC transporter substrate-binding protein, at 1,027,872 to 1,029,089 _RS04445 BPHYT_RS04450: BPHYT_RS04450 - UDP-glucose 4-epimerase, at 1,029,314 to 1,030,336 _RS04450 BPHYT_RS04455: BPHYT_RS04455 - glycosyl transferase, at 1,030,349 to 1,031,497 _RS04455 Position (kb) 1029 1030 1031Strain fitness (log2 ratio) -3 -2 -1 0 1at 1028.555 kb on - strand, within BPHYT_RS04445at 1028.666 kb on - strand, within BPHYT_RS04445at 1028.666 kb on - strand, within BPHYT_RS04445at 1028.666 kb on - strand, within BPHYT_RS04445at 1028.789 kb on - strand, within BPHYT_RS04445at 1029.025 kb on + strandat 1029.197 kb on - strandat 1029.219 kb on + strandat 1029.528 kb on + strand, within BPHYT_RS04450at 1029.529 kb on - strand, within BPHYT_RS04450at 1029.631 kb on - strand, within BPHYT_RS04450at 1030.028 kb on - strand, within BPHYT_RS04450at 1030.036 kb on - strand, within BPHYT_RS04450at 1030.238 kb on + strandat 1030.356 kb on - strandat 1030.852 kb on - strand, within BPHYT_RS04455at 1031.043 kb on - strand, within BPHYT_RS04455

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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1,028,555 - BPHYT_RS04445 0.56 -0.4
1,028,666 - BPHYT_RS04445 0.65 +0.2
1,028,666 - BPHYT_RS04445 0.65 +0.2
1,028,666 - BPHYT_RS04445 0.65 -0.7
1,028,789 - BPHYT_RS04445 0.75 -1.0
1,029,025 + +0.0
1,029,197 - -0.3
1,029,219 + +0.6
1,029,528 + BPHYT_RS04450 0.21 +1.4
1,029,529 - BPHYT_RS04450 0.21 +1.0
1,029,631 - BPHYT_RS04450 0.31 +0.0
1,030,028 - BPHYT_RS04450 0.70 +1.4
1,030,036 - BPHYT_RS04450 0.71 +0.3
1,030,238 + -1.2
1,030,356 - -1.0
1,030,852 - BPHYT_RS04455 0.44 -0.7
1,031,043 - BPHYT_RS04455 0.60 -2.9

Or see this region's nucleotide sequence