Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02960 and BPHYT_RS02965 are separated by 184 nucleotides BPHYT_RS02965 and BPHYT_RS02970 are separated by 37 nucleotides
BPHYT_RS02960: BPHYT_RS02960 - peptidase, at 657,164 to 658,390
_RS02960
BPHYT_RS02965: BPHYT_RS02965 - membrane protein, at 658,575 to 659,660
_RS02965
BPHYT_RS02970: BPHYT_RS02970 - excinuclease ABC subunit A, at 659,698 to 662,571
_RS02970
Position (kb) 658
659
660 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 657.799 kb on + strand, within BPHYT_RS02960 at 657.799 kb on + strand, within BPHYT_RS02960 at 657.799 kb on + strand, within BPHYT_RS02960 at 657.800 kb on - strand, within BPHYT_RS02960 at 657.800 kb on - strand, within BPHYT_RS02960 at 657.800 kb on - strand, within BPHYT_RS02960 at 657.800 kb on - strand, within BPHYT_RS02960 at 657.801 kb on + strand, within BPHYT_RS02960 at 657.978 kb on + strand, within BPHYT_RS02960 at 658.142 kb on - strand, within BPHYT_RS02960 at 658.142 kb on - strand, within BPHYT_RS02960 at 658.170 kb on + strand, within BPHYT_RS02960 at 658.171 kb on - strand, within BPHYT_RS02960 at 658.171 kb on - strand, within BPHYT_RS02960 at 658.610 kb on + strand at 658.613 kb on - strand at 659.066 kb on + strand, within BPHYT_RS02965 at 659.066 kb on + strand, within BPHYT_RS02965 at 659.067 kb on - strand, within BPHYT_RS02965 at 659.540 kb on + strand, within BPHYT_RS02965 at 659.543 kb on - strand, within BPHYT_RS02965 at 659.614 kb on - strand at 659.666 kb on - strand at 659.666 kb on - strand at 659.696 kb on - strand at 659.700 kb on - strand at 659.892 kb on + strand at 659.895 kb on + strand at 659.895 kb on + strand at 659.896 kb on - strand at 659.977 kb on + strand at 659.977 kb on + strand at 659.977 kb on + strand at 659.977 kb on + strand at 659.977 kb on + strand at 659.977 kb on + strand at 659.977 kb on + strand at 659.978 kb on - strand at 659.981 kb on + strand at 659.981 kb on + strand at 659.981 kb on + strand at 659.981 kb on + strand at 659.981 kb on + strand at 659.981 kb on + strand at 659.981 kb on + strand at 659.982 kb on - strand at 659.982 kb on - strand at 659.982 kb on - strand at 659.983 kb on + strand at 659.983 kb on + strand at 660.022 kb on + strand, within BPHYT_RS02970 at 660.022 kb on + strand, within BPHYT_RS02970 at 660.022 kb on + strand, within BPHYT_RS02970 at 660.022 kb on + strand, within BPHYT_RS02970 at 660.084 kb on - strand, within BPHYT_RS02970 at 660.152 kb on + strand, within BPHYT_RS02970 at 660.152 kb on + strand, within BPHYT_RS02970 at 660.203 kb on + strand, within BPHYT_RS02970 at 660.204 kb on - strand, within BPHYT_RS02970 at 660.230 kb on + strand, within BPHYT_RS02970 at 660.263 kb on + strand, within BPHYT_RS02970 at 660.263 kb on + strand, within BPHYT_RS02970 at 660.263 kb on + strand at 660.264 kb on - strand, within BPHYT_RS02970 at 660.337 kb on + strand, within BPHYT_RS02970 at 660.338 kb on - strand, within BPHYT_RS02970 at 660.431 kb on + strand, within BPHYT_RS02970 at 660.431 kb on + strand, within BPHYT_RS02970 at 660.431 kb on + strand, within BPHYT_RS02970 at 660.431 kb on + strand, within BPHYT_RS02970 at 660.432 kb on - strand, within BPHYT_RS02970 at 660.575 kb on + strand, within BPHYT_RS02970
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 657,799 + BPHYT_RS02960 0.52 -1.7 657,799 + BPHYT_RS02960 0.52 -1.0 657,799 + BPHYT_RS02960 0.52 +1.1 657,800 - BPHYT_RS02960 0.52 +1.0 657,800 - BPHYT_RS02960 0.52 +1.2 657,800 - BPHYT_RS02960 0.52 +0.2 657,800 - BPHYT_RS02960 0.52 -0.7 657,801 + BPHYT_RS02960 0.52 -0.2 657,978 + BPHYT_RS02960 0.66 -2.1 658,142 - BPHYT_RS02960 0.80 -0.1 658,142 - BPHYT_RS02960 0.80 -0.1 658,170 + BPHYT_RS02960 0.82 +0.0 658,171 - BPHYT_RS02960 0.82 -0.1 658,171 - BPHYT_RS02960 0.82 +0.1 658,610 + -2.3 658,613 - -4.4 659,066 + BPHYT_RS02965 0.45 -4.2 659,066 + BPHYT_RS02965 0.45 -1.4 659,067 - BPHYT_RS02965 0.45 -1.4 659,540 + BPHYT_RS02965 0.89 -3.6 659,543 - BPHYT_RS02965 0.89 -1.9 659,614 - -2.4 659,666 - +1.6 659,666 - +2.1 659,696 - -0.1 659,700 - +1.5 659,892 + -1.0 659,895 + -1.7 659,895 + +0.3 659,896 - -1.9 659,977 + +0.7 659,977 + +0.1 659,977 + +0.7 659,977 + +0.7 659,977 + +0.6 659,977 + +0.3 659,977 + +0.3 659,978 - -2.7 659,981 + +0.3 659,981 + +0.3 659,981 + -0.1 659,981 + +0.7 659,981 + +0.8 659,981 + +0.1 659,981 + +0.5 659,982 - -0.4 659,982 - +0.9 659,982 - -0.4 659,983 + +0.9 659,983 + -0.8 660,022 + BPHYT_RS02970 0.11 +1.6 660,022 + BPHYT_RS02970 0.11 +2.2 660,022 + BPHYT_RS02970 0.11 +1.5 660,022 + BPHYT_RS02970 0.11 +1.7 660,084 - BPHYT_RS02970 0.13 -0.9 660,152 + BPHYT_RS02970 0.16 +1.8 660,152 + BPHYT_RS02970 0.16 +0.9 660,203 + BPHYT_RS02970 0.18 +1.2 660,204 - BPHYT_RS02970 0.18 -1.1 660,230 + BPHYT_RS02970 0.19 +0.2 660,263 + BPHYT_RS02970 0.20 +0.9 660,263 + BPHYT_RS02970 0.20 +1.0 660,263 + +1.9 660,264 - BPHYT_RS02970 0.20 -1.3 660,337 + BPHYT_RS02970 0.22 +1.1 660,338 - BPHYT_RS02970 0.22 -0.0 660,431 + BPHYT_RS02970 0.26 +0.2 660,431 + BPHYT_RS02970 0.26 +1.4 660,431 + BPHYT_RS02970 0.26 +1.3 660,431 + BPHYT_RS02970 0.26 -1.6 660,432 - BPHYT_RS02970 0.26 -2.1 660,575 + BPHYT_RS02970 0.31 +0.7
Or see this region's nucleotide sequence