Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02645 and BPHYT_RS02650 are separated by 114 nucleotides BPHYT_RS02650 and BPHYT_RS02655 are separated by 42 nucleotides BPHYT_RS02655 and BPHYT_RS02660 are separated by 36 nucleotides BPHYT_RS02660 and BPHYT_RS02665 are separated by 132 nucleotides
BPHYT_RS02645: BPHYT_RS02645 - cytochrome oxidase subunit I, at 597,377 to 598,990
_RS02645
BPHYT_RS02650: BPHYT_RS02650 - membrane protein, at 599,105 to 599,239
_RS02650
BPHYT_RS02655: BPHYT_RS02655 - cytochrome C oxidase assembly protein, at 599,282 to 599,920
_RS02655
BPHYT_RS02660: BPHYT_RS02660 - membrane protein, at 599,957 to 600,166
_RS02660
BPHYT_RS02665: BPHYT_RS02665 - MFS transporter, at 600,299 to 601,156
_RS02665
Position (kb) 599
600
601 Strain fitness (log2 ratio) -2
-1
0
1
2 at 599.036 kb on + strand at 599.036 kb on + strand at 599.075 kb on + strand at 599.132 kb on - strand, within BPHYT_RS02650 at 599.450 kb on - strand, within BPHYT_RS02655 at 599.470 kb on + strand, within BPHYT_RS02655 at 599.594 kb on + strand, within BPHYT_RS02655 at 599.594 kb on + strand, within BPHYT_RS02655 at 599.649 kb on - strand, within BPHYT_RS02655 at 599.673 kb on - strand, within BPHYT_RS02655 at 599.755 kb on - strand, within BPHYT_RS02655 at 600.124 kb on + strand, within BPHYT_RS02660 at 600.156 kb on + strand at 600.159 kb on + strand at 600.184 kb on + strand at 600.251 kb on + strand at 600.252 kb on - strand at 600.323 kb on + strand at 600.323 kb on + strand at 600.326 kb on + strand at 600.466 kb on + strand, within BPHYT_RS02665 at 600.518 kb on + strand, within BPHYT_RS02665 at 600.518 kb on + strand, within BPHYT_RS02665 at 600.519 kb on - strand, within BPHYT_RS02665 at 600.519 kb on - strand, within BPHYT_RS02665 at 600.519 kb on - strand, within BPHYT_RS02665 at 600.614 kb on + strand, within BPHYT_RS02665 at 600.614 kb on + strand, within BPHYT_RS02665 at 600.614 kb on + strand, within BPHYT_RS02665 at 600.615 kb on - strand, within BPHYT_RS02665 at 600.615 kb on - strand, within BPHYT_RS02665 at 600.656 kb on + strand, within BPHYT_RS02665 at 600.657 kb on - strand, within BPHYT_RS02665 at 600.918 kb on - strand, within BPHYT_RS02665 at 600.918 kb on - strand, within BPHYT_RS02665 at 600.953 kb on + strand, within BPHYT_RS02665 at 600.954 kb on - strand, within BPHYT_RS02665 at 600.954 kb on - strand, within BPHYT_RS02665 at 600.954 kb on - strand at 601.039 kb on + strand, within BPHYT_RS02665 at 601.057 kb on + strand at 601.058 kb on - strand at 601.134 kb on - strand at 601.134 kb on - strand at 601.134 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 599,036 + +0.3 599,036 + -0.1 599,075 + +1.3 599,132 - BPHYT_RS02650 0.20 +0.1 599,450 - BPHYT_RS02655 0.26 -1.1 599,470 + BPHYT_RS02655 0.29 +0.3 599,594 + BPHYT_RS02655 0.49 +0.4 599,594 + BPHYT_RS02655 0.49 +0.5 599,649 - BPHYT_RS02655 0.57 +0.5 599,673 - BPHYT_RS02655 0.61 -0.0 599,755 - BPHYT_RS02655 0.74 -1.1 600,124 + BPHYT_RS02660 0.80 +1.3 600,156 + +0.7 600,159 + +0.5 600,184 + -0.0 600,251 + -0.1 600,252 - -1.1 600,323 + -0.3 600,323 + +0.4 600,326 + -0.6 600,466 + BPHYT_RS02665 0.19 -0.0 600,518 + BPHYT_RS02665 0.26 -0.1 600,518 + BPHYT_RS02665 0.26 -0.6 600,519 - BPHYT_RS02665 0.26 -0.3 600,519 - BPHYT_RS02665 0.26 -0.0 600,519 - BPHYT_RS02665 0.26 -0.6 600,614 + BPHYT_RS02665 0.37 -0.2 600,614 + BPHYT_RS02665 0.37 +0.9 600,614 + BPHYT_RS02665 0.37 -0.1 600,615 - BPHYT_RS02665 0.37 -0.0 600,615 - BPHYT_RS02665 0.37 +1.0 600,656 + BPHYT_RS02665 0.42 +0.4 600,657 - BPHYT_RS02665 0.42 +0.4 600,918 - BPHYT_RS02665 0.72 -2.5 600,918 - BPHYT_RS02665 0.72 +0.3 600,953 + BPHYT_RS02665 0.76 +0.4 600,954 - BPHYT_RS02665 0.76 -0.0 600,954 - BPHYT_RS02665 0.76 +0.2 600,954 - +0.9 601,039 + BPHYT_RS02665 0.86 +0.3 601,057 + -0.6 601,058 - +1.9 601,134 - -0.8 601,134 - +0.1 601,134 - -0.5
Or see this region's nucleotide sequence