Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02290 and BPHYT_RS02295 are separated by 442 nucleotides BPHYT_RS02295 and BPHYT_RS02300 are separated by 142 nucleotides BPHYT_RS02300 and BPHYT_RS02305 are separated by 159 nucleotides
BPHYT_RS02290: BPHYT_RS02290 - arginine--tRNA ligase, at 524,071 to 525,861
_RS02290
BPHYT_RS02295: BPHYT_RS02295 - hypothetical protein, at 526,304 to 526,630
_RS02295
BPHYT_RS02300: BPHYT_RS02300 - acid shock protein, at 526,773 to 526,982
_RS02300
BPHYT_RS02305: BPHYT_RS02305 - methionine synthase, at 527,142 to 529,850
_RS02305
Position (kb) 526
527 Strain fitness (log2 ratio) -2
-1
0
1
2 at 526.075 kb on - strand at 526.172 kb on + strand at 526.172 kb on + strand at 526.172 kb on + strand at 526.172 kb on + strand at 526.172 kb on + strand at 526.173 kb on - strand at 526.173 kb on - strand at 526.173 kb on - strand at 526.173 kb on - strand at 526.175 kb on + strand at 526.175 kb on + strand at 526.175 kb on + strand at 526.175 kb on + strand at 526.175 kb on + strand at 526.175 kb on + strand at 526.176 kb on - strand at 526.176 kb on - strand at 526.313 kb on - strand at 526.313 kb on - strand at 526.313 kb on - strand at 526.367 kb on + strand, within BPHYT_RS02295 at 526.367 kb on + strand, within BPHYT_RS02295 at 526.367 kb on + strand, within BPHYT_RS02295 at 526.368 kb on - strand, within BPHYT_RS02295 at 526.719 kb on + strand at 526.720 kb on - strand at 526.775 kb on - strand at 526.877 kb on - strand, within BPHYT_RS02300 at 527.011 kb on - strand at 527.011 kb on - strand at 527.063 kb on - strand at 527.091 kb on + strand at 527.143 kb on + strand at 527.203 kb on + strand at 527.204 kb on - strand at 527.245 kb on + strand at 527.245 kb on + strand at 527.246 kb on - strand at 527.246 kb on - strand at 527.246 kb on - strand at 527.246 kb on - strand at 527.419 kb on + strand, within BPHYT_RS02305 at 527.419 kb on + strand, within BPHYT_RS02305 at 527.560 kb on + strand, within BPHYT_RS02305 at 527.638 kb on + strand, within BPHYT_RS02305 at 527.639 kb on - strand, within BPHYT_RS02305 at 527.782 kb on + strand, within BPHYT_RS02305
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 526,075 - +0.5 526,172 + +0.7 526,172 + -1.7 526,172 + -0.1 526,172 + +0.1 526,172 + -0.5 526,173 - -0.6 526,173 - -0.4 526,173 - +0.3 526,173 - +0.7 526,175 + -0.3 526,175 + -0.1 526,175 + -1.3 526,175 + +0.3 526,175 + +0.6 526,175 + +0.3 526,176 - -0.7 526,176 - -0.2 526,313 - +0.2 526,313 - +1.0 526,313 - +0.5 526,367 + BPHYT_RS02295 0.19 +0.1 526,367 + BPHYT_RS02295 0.19 +1.8 526,367 + BPHYT_RS02295 0.19 +0.2 526,368 - BPHYT_RS02295 0.20 +1.0 526,719 + +0.4 526,720 - -0.0 526,775 - +0.4 526,877 - BPHYT_RS02300 0.50 -1.2 527,011 - -0.2 527,011 - -0.1 527,063 - -0.9 527,091 + -0.2 527,143 + -0.2 527,203 + -0.3 527,204 - -2.0 527,245 + -0.3 527,245 + +0.3 527,246 - -0.5 527,246 - +0.1 527,246 - -1.0 527,246 - -0.2 527,419 + BPHYT_RS02305 0.10 -0.9 527,419 + BPHYT_RS02305 0.10 -0.6 527,560 + BPHYT_RS02305 0.15 -0.1 527,638 + BPHYT_RS02305 0.18 +0.7 527,639 - BPHYT_RS02305 0.18 +0.7 527,782 + BPHYT_RS02305 0.24 -0.6
Or see this region's nucleotide sequence