Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02115 and BPHYT_RS02120 overlap by 4 nucleotides BPHYT_RS02120 and BPHYT_RS02125 are separated by 19 nucleotides BPHYT_RS02125 and BPHYT_RS02130 are separated by 54 nucleotides BPHYT_RS02130 and BPHYT_RS02135 are separated by 60 nucleotides
BPHYT_RS02115: BPHYT_RS02115 - acyl dehydratase, at 486,833 to 487,324
_RS02115
BPHYT_RS02120: BPHYT_RS02120 - hypothetical protein, at 487,321 to 487,545
_RS02120
BPHYT_RS02125: BPHYT_RS02125 - prolyl-tRNA synthetase, at 487,565 to 488,086
_RS02125
BPHYT_RS02130: BPHYT_RS02130 - hydroxymethylglutaryl-CoA lyase, at 488,141 to 489,067
_RS02130
BPHYT_RS02135: BPHYT_RS02135 - glyoxylate reductase, at 489,128 to 490,069
_RS02135
Position (kb) 488
489
490 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 487.440 kb on + strand, within BPHYT_RS02120 at 487.441 kb on - strand, within BPHYT_RS02120 at 488.299 kb on - strand, within BPHYT_RS02130 at 488.299 kb on - strand, within BPHYT_RS02130 at 489.079 kb on + strand at 489.080 kb on - strand at 489.124 kb on - strand at 489.405 kb on + strand, within BPHYT_RS02135 at 489.405 kb on + strand, within BPHYT_RS02135 at 489.763 kb on - strand, within BPHYT_RS02135 at 489.818 kb on - strand, within BPHYT_RS02135
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 487,440 + BPHYT_RS02120 0.53 -3.5 487,441 - BPHYT_RS02120 0.53 -0.0 488,299 - BPHYT_RS02130 0.17 -2.5 488,299 - BPHYT_RS02130 0.17 -2.2 489,079 + -0.0 489,080 - -0.8 489,124 - -0.4 489,405 + BPHYT_RS02135 0.29 -1.1 489,405 + BPHYT_RS02135 0.29 -1.7 489,763 - BPHYT_RS02135 0.67 -1.8 489,818 - BPHYT_RS02135 0.73 -2.0
Or see this region's nucleotide sequence