Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01240 and BPHYT_RS01245 are separated by 95 nucleotides BPHYT_RS01245 and BPHYT_RS01250 are separated by 54 nucleotides BPHYT_RS01250 and BPHYT_RS01255 are separated by 285 nucleotides BPHYT_RS01255 and BPHYT_RS01260 are separated by 215 nucleotides
BPHYT_RS01240: BPHYT_RS01240 - hypothetical protein, at 285,205 to 285,423
_RS01240
BPHYT_RS01245: BPHYT_RS01245 - hypothetical protein, at 285,519 to 286,262
_RS01245
BPHYT_RS01250: BPHYT_RS01250 - integrase, at 286,317 to 287,315
_RS01250
BPHYT_RS01255: BPHYT_RS01255 - AsnC family transcriptional regulator, at 287,601 to 288,062
_RS01255
BPHYT_RS01260: BPHYT_RS01260 - hypothetical protein, at 288,278 to 289,408
_RS01260
Position (kb) 286
287
288 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 285.381 kb on - strand, within BPHYT_RS01240 at 285.427 kb on - strand at 285.527 kb on + strand at 285.591 kb on + strand at 285.693 kb on + strand, within BPHYT_RS01245 at 285.694 kb on - strand, within BPHYT_RS01245 at 285.951 kb on + strand, within BPHYT_RS01245 at 285.951 kb on + strand, within BPHYT_RS01245 at 285.952 kb on - strand, within BPHYT_RS01245 at 285.952 kb on - strand, within BPHYT_RS01245 at 286.058 kb on - strand, within BPHYT_RS01245 at 286.058 kb on - strand, within BPHYT_RS01245 at 286.171 kb on + strand, within BPHYT_RS01245 at 286.172 kb on - strand, within BPHYT_RS01245 at 286.260 kb on + strand at 286.516 kb on + strand, within BPHYT_RS01250 at 286.516 kb on + strand, within BPHYT_RS01250 at 286.522 kb on - strand, within BPHYT_RS01250 at 286.701 kb on + strand, within BPHYT_RS01250 at 286.702 kb on - strand, within BPHYT_RS01250 at 286.934 kb on + strand, within BPHYT_RS01250 at 286.935 kb on - strand, within BPHYT_RS01250 at 287.196 kb on - strand, within BPHYT_RS01250 at 287.228 kb on + strand at 287.228 kb on + strand at 287.229 kb on - strand at 287.229 kb on - strand at 287.533 kb on - strand at 287.652 kb on - strand, within BPHYT_RS01255 at 287.717 kb on - strand, within BPHYT_RS01255 at 287.717 kb on - strand, within BPHYT_RS01255 at 287.756 kb on - strand, within BPHYT_RS01255 at 287.852 kb on - strand, within BPHYT_RS01255 at 287.973 kb on + strand, within BPHYT_RS01255 at 288.074 kb on + strand at 288.075 kb on - strand at 288.142 kb on - strand at 288.142 kb on - strand at 288.175 kb on + strand at 288.301 kb on + strand at 288.301 kb on + strand at 288.301 kb on + strand at 288.302 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 285,381 - BPHYT_RS01240 0.80 -1.3 285,427 - -1.4 285,527 + -1.1 285,591 + +0.4 285,693 + BPHYT_RS01245 0.23 +0.7 285,694 - BPHYT_RS01245 0.24 +0.2 285,951 + BPHYT_RS01245 0.58 +0.2 285,951 + BPHYT_RS01245 0.58 -0.5 285,952 - BPHYT_RS01245 0.58 +0.3 285,952 - BPHYT_RS01245 0.58 -0.5 286,058 - BPHYT_RS01245 0.72 -0.1 286,058 - BPHYT_RS01245 0.72 -0.3 286,171 + BPHYT_RS01245 0.88 -0.8 286,172 - BPHYT_RS01245 0.88 +0.5 286,260 + -0.0 286,516 + BPHYT_RS01250 0.20 -0.8 286,516 + BPHYT_RS01250 0.20 -0.3 286,522 - BPHYT_RS01250 0.21 -1.4 286,701 + BPHYT_RS01250 0.38 -0.2 286,702 - BPHYT_RS01250 0.39 +0.2 286,934 + BPHYT_RS01250 0.62 +0.5 286,935 - BPHYT_RS01250 0.62 -0.3 287,196 - BPHYT_RS01250 0.88 +0.4 287,228 + +0.5 287,228 + -3.0 287,229 - -0.1 287,229 - -0.1 287,533 - -0.7 287,652 - BPHYT_RS01255 0.11 +0.1 287,717 - BPHYT_RS01255 0.25 +0.6 287,717 - BPHYT_RS01255 0.25 -0.5 287,756 - BPHYT_RS01255 0.34 +0.2 287,852 - BPHYT_RS01255 0.54 +1.2 287,973 + BPHYT_RS01255 0.81 +0.9 288,074 + +0.4 288,075 - -0.5 288,142 - +0.1 288,142 - +0.2 288,175 + +0.1 288,301 + +0.4 288,301 + -1.7 288,301 + -1.2 288,302 - -3.1
Or see this region's nucleotide sequence