Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00270 and BPHYT_RS00275 are separated by 37 nucleotides BPHYT_RS00275 and BPHYT_RS00280 are separated by 2 nucleotides BPHYT_RS00280 and BPHYT_RS00285 are separated by 9 nucleotides BPHYT_RS00285 and BPHYT_RS00290 overlap by 16 nucleotides
BPHYT_RS00270: BPHYT_RS00270 - branched-chain amino acid ABC transporter, at 69,485 to 70,186
_RS00270
BPHYT_RS00275: BPHYT_RS00275 - urease subunit beta, at 70,224 to 70,910
_RS00275
BPHYT_RS00280: BPHYT_RS00280 - urease subunit alpha, at 70,913 to 72,628
_RS00280
BPHYT_RS00285: BPHYT_RS00285 - urease accessory protein UreG, at 72,638 to 73,252
_RS00285
BPHYT_RS00290: BPHYT_RS00290 - hypothetical protein, at 73,237 to 74,064
_RS00290
Position (kb) 70
71
72
73 Strain fitness (log2 ratio) -2
-1
0
1
2 at 70.094 kb on + strand, within BPHYT_RS00270 at 70.095 kb on - strand, within BPHYT_RS00270 at 70.306 kb on + strand, within BPHYT_RS00275 at 70.307 kb on - strand, within BPHYT_RS00275 at 70.460 kb on - strand, within BPHYT_RS00275 at 70.460 kb on - strand, within BPHYT_RS00275 at 70.460 kb on - strand, within BPHYT_RS00275 at 70.607 kb on + strand, within BPHYT_RS00275 at 70.608 kb on - strand, within BPHYT_RS00275 at 70.683 kb on - strand, within BPHYT_RS00275 at 70.772 kb on + strand, within BPHYT_RS00275 at 70.772 kb on + strand, within BPHYT_RS00275 at 70.772 kb on + strand, within BPHYT_RS00275 at 70.773 kb on - strand, within BPHYT_RS00275 at 70.908 kb on + strand at 70.908 kb on + strand at 70.909 kb on - strand at 70.909 kb on - strand at 70.909 kb on - strand at 70.909 kb on - strand at 70.941 kb on - strand at 71.200 kb on + strand, within BPHYT_RS00280 at 71.200 kb on + strand, within BPHYT_RS00280 at 71.733 kb on - strand, within BPHYT_RS00280 at 71.733 kb on - strand, within BPHYT_RS00280 at 71.842 kb on + strand, within BPHYT_RS00280 at 71.842 kb on + strand, within BPHYT_RS00280 at 71.843 kb on - strand at 72.062 kb on - strand, within BPHYT_RS00280 at 72.062 kb on - strand, within BPHYT_RS00280 at 72.062 kb on - strand, within BPHYT_RS00280 at 72.131 kb on + strand, within BPHYT_RS00280 at 72.134 kb on - strand, within BPHYT_RS00280 at 72.143 kb on + strand at 72.144 kb on - strand, within BPHYT_RS00280 at 72.614 kb on + strand at 72.615 kb on - strand at 72.615 kb on - strand at 72.615 kb on - strand at 72.626 kb on + strand at 72.626 kb on + strand at 72.627 kb on - strand at 72.649 kb on - strand at 73.024 kb on + strand, within BPHYT_RS00285 at 73.024 kb on + strand, within BPHYT_RS00285 at 73.025 kb on - strand, within BPHYT_RS00285 at 73.097 kb on + strand, within BPHYT_RS00285 at 73.097 kb on + strand, within BPHYT_RS00285 at 73.097 kb on + strand, within BPHYT_RS00285 at 73.098 kb on - strand, within BPHYT_RS00285 at 73.098 kb on - strand, within BPHYT_RS00285 at 73.135 kb on - strand, within BPHYT_RS00285 at 73.240 kb on + strand at 73.241 kb on - strand at 73.384 kb on + strand, within BPHYT_RS00290 at 73.385 kb on - strand, within BPHYT_RS00290 at 73.463 kb on + strand, within BPHYT_RS00290 at 73.464 kb on - strand, within BPHYT_RS00290
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 70,094 + BPHYT_RS00270 0.87 -0.0 70,095 - BPHYT_RS00270 0.87 -1.0 70,306 + BPHYT_RS00275 0.12 -0.7 70,307 - BPHYT_RS00275 0.12 +0.5 70,460 - BPHYT_RS00275 0.34 -0.8 70,460 - BPHYT_RS00275 0.34 -0.6 70,460 - BPHYT_RS00275 0.34 -0.4 70,607 + BPHYT_RS00275 0.56 +0.4 70,608 - BPHYT_RS00275 0.56 -0.5 70,683 - BPHYT_RS00275 0.67 +1.3 70,772 + BPHYT_RS00275 0.80 +0.7 70,772 + BPHYT_RS00275 0.80 -1.1 70,772 + BPHYT_RS00275 0.80 -1.4 70,773 - BPHYT_RS00275 0.80 -0.3 70,908 + +0.1 70,908 + -2.3 70,909 - -0.3 70,909 - -0.2 70,909 - +0.8 70,909 - -0.5 70,941 - -1.3 71,200 + BPHYT_RS00280 0.17 +0.2 71,200 + BPHYT_RS00280 0.17 -0.2 71,733 - BPHYT_RS00280 0.48 -0.3 71,733 - BPHYT_RS00280 0.48 +0.7 71,842 + BPHYT_RS00280 0.54 +2.3 71,842 + BPHYT_RS00280 0.54 -0.2 71,843 - +0.9 72,062 - BPHYT_RS00280 0.67 +1.9 72,062 - BPHYT_RS00280 0.67 +0.3 72,062 - BPHYT_RS00280 0.67 +0.5 72,131 + BPHYT_RS00280 0.71 +0.3 72,134 - BPHYT_RS00280 0.71 +0.6 72,143 + -0.7 72,144 - BPHYT_RS00280 0.72 -0.2 72,614 + -0.2 72,615 - +0.4 72,615 - +0.9 72,615 - +0.8 72,626 + +0.4 72,626 + -0.4 72,627 - +0.2 72,649 - -0.1 73,024 + BPHYT_RS00285 0.63 -0.0 73,024 + BPHYT_RS00285 0.63 +0.4 73,025 - BPHYT_RS00285 0.63 +0.6 73,097 + BPHYT_RS00285 0.75 +1.6 73,097 + BPHYT_RS00285 0.75 -1.2 73,097 + BPHYT_RS00285 0.75 -0.8 73,098 - BPHYT_RS00285 0.75 +1.3 73,098 - BPHYT_RS00285 0.75 -0.7 73,135 - BPHYT_RS00285 0.81 -0.0 73,240 + -0.2 73,241 - -0.1 73,384 + BPHYT_RS00290 0.18 -0.7 73,385 - BPHYT_RS00290 0.18 -0.1 73,463 + BPHYT_RS00290 0.27 -0.5 73,464 - BPHYT_RS00290 0.27 +0.2
Or see this region's nucleotide sequence