Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS32055

Experiment: L-Isoleucine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS32050 and RR42_RS32055 are separated by 128 nucleotidesRR42_RS32055 and RR42_RS32060 are separated by 137 nucleotidesRR42_RS32060 and RR42_RS32065 are separated by 3 nucleotides RR42_RS32050: RR42_RS32050 - AraC family transcriptional regulator, at 2,567,432 to 2,568,625 _RS32050 RR42_RS32055: RR42_RS32055 - 3-carboxymuconate cycloisomerase PcaB (EC 5.5.1.2) (from data), at 2,568,754 to 2,570,079 _RS32055 RR42_RS32060: RR42_RS32060 - protocatechuate 3,4-dioxygenase, at 2,570,217 to 2,570,795 _RS32060 RR42_RS32065: RR42_RS32065 - protocatechuate 3,4-dioxygenase, beta subunit (EC 1.13.11.3) (from data), at 2,570,799 to 2,571,524 _RS32065 Position (kb) 2568 2569 2570 2571Strain fitness (log2 ratio) -1 0 1 2at 2568.084 kb on + strand, within RR42_RS32050at 2568.589 kb on - strandat 2568.589 kb on - strandat 2568.589 kb on - strandat 2568.660 kb on + strandat 2568.661 kb on - strandat 2568.661 kb on - strandat 2568.665 kb on + strandat 2568.804 kb on - strandat 2568.804 kb on - strandat 2569.128 kb on + strand, within RR42_RS32055at 2569.129 kb on - strand, within RR42_RS32055at 2569.397 kb on - strand, within RR42_RS32055at 2569.407 kb on + strand, within RR42_RS32055at 2569.408 kb on - strand, within RR42_RS32055at 2569.408 kb on - strand, within RR42_RS32055at 2570.377 kb on + strand, within RR42_RS32060at 2570.377 kb on + strand, within RR42_RS32060at 2570.377 kb on + strand, within RR42_RS32060at 2570.377 kb on + strand, within RR42_RS32060at 2570.377 kb on + strand, within RR42_RS32060at 2570.378 kb on - strand, within RR42_RS32060at 2570.380 kb on - strand, within RR42_RS32060at 2570.380 kb on - strand, within RR42_RS32060at 2570.415 kb on - strand, within RR42_RS32060at 2570.415 kb on - strand, within RR42_RS32060at 2570.432 kb on + strand, within RR42_RS32060at 2570.432 kb on + strand, within RR42_RS32060at 2570.432 kb on + strand, within RR42_RS32060at 2570.759 kb on - strandat 2570.789 kb on - strandat 2570.800 kb on + strandat 2570.800 kb on + strandat 2570.801 kb on - strandat 2570.837 kb on + strandat 2570.974 kb on + strand, within RR42_RS32065at 2570.974 kb on + strand, within RR42_RS32065at 2570.974 kb on + strand, within RR42_RS32065at 2570.974 kb on + strand, within RR42_RS32065at 2570.975 kb on - strand, within RR42_RS32065at 2570.975 kb on - strand, within RR42_RS32065at 2570.975 kb on - strand, within RR42_RS32065at 2570.975 kb on - strand, within RR42_RS32065at 2571.001 kb on + strand, within RR42_RS32065at 2571.002 kb on - strand, within RR42_RS32065at 2571.029 kb on + strand, within RR42_RS32065at 2571.030 kb on - strand, within RR42_RS32065at 2571.030 kb on - strand, within RR42_RS32065at 2571.030 kb on - strand, within RR42_RS32065at 2571.030 kb on - strand, within RR42_RS32065at 2571.030 kb on - strand, within RR42_RS32065

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (N)
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2,568,084 + RR42_RS32050 0.55 -0.4
2,568,589 - -0.0
2,568,589 - -0.1
2,568,589 - -0.4
2,568,660 + +0.1
2,568,661 - -0.2
2,568,661 - -0.2
2,568,665 + +0.1
2,568,804 - +1.2
2,568,804 - -0.4
2,569,128 + RR42_RS32055 0.28 -0.9
2,569,129 - RR42_RS32055 0.28 -1.0
2,569,397 - RR42_RS32055 0.48 +0.3
2,569,407 + RR42_RS32055 0.49 -1.0
2,569,408 - RR42_RS32055 0.49 -1.3
2,569,408 - RR42_RS32055 0.49 -0.4
2,570,377 + RR42_RS32060 0.28 +0.5
2,570,377 + RR42_RS32060 0.28 -0.6
2,570,377 + RR42_RS32060 0.28 -0.1
2,570,377 + RR42_RS32060 0.28 -0.3
2,570,377 + RR42_RS32060 0.28 -0.9
2,570,378 - RR42_RS32060 0.28 -0.2
2,570,380 - RR42_RS32060 0.28 +0.1
2,570,380 - RR42_RS32060 0.28 -0.1
2,570,415 - RR42_RS32060 0.34 +1.6
2,570,415 - RR42_RS32060 0.34 +0.3
2,570,432 + RR42_RS32060 0.37 +0.0
2,570,432 + RR42_RS32060 0.37 -0.3
2,570,432 + RR42_RS32060 0.37 +0.1
2,570,759 - +0.2
2,570,789 - -0.1
2,570,800 + -0.9
2,570,800 + -0.3
2,570,801 - -0.4
2,570,837 + -0.7
2,570,974 + RR42_RS32065 0.24 -0.0
2,570,974 + RR42_RS32065 0.24 -0.4
2,570,974 + RR42_RS32065 0.24 +1.2
2,570,974 + RR42_RS32065 0.24 -0.5
2,570,975 - RR42_RS32065 0.24 -0.1
2,570,975 - RR42_RS32065 0.24 +0.1
2,570,975 - RR42_RS32065 0.24 -0.1
2,570,975 - RR42_RS32065 0.24 +1.0
2,571,001 + RR42_RS32065 0.28 -0.0
2,571,002 - RR42_RS32065 0.28 +1.0
2,571,029 + RR42_RS32065 0.32 -0.1
2,571,030 - RR42_RS32065 0.32 +1.0
2,571,030 - RR42_RS32065 0.32 +2.1
2,571,030 - RR42_RS32065 0.32 -0.0
2,571,030 - RR42_RS32065 0.32 +0.2
2,571,030 - RR42_RS32065 0.32 +1.1

Or see this region's nucleotide sequence