Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32050 and RR42_RS32055 are separated by 128 nucleotides RR42_RS32055 and RR42_RS32060 are separated by 137 nucleotides RR42_RS32060 and RR42_RS32065 are separated by 3 nucleotides
RR42_RS32050: RR42_RS32050 - AraC family transcriptional regulator, at 2,567,432 to 2,568,625
_RS32050
RR42_RS32055: RR42_RS32055 - 3-carboxymuconate cycloisomerase PcaB (EC 5.5.1.2) (from data) , at 2,568,754 to 2,570,079
_RS32055
RR42_RS32060: RR42_RS32060 - protocatechuate 3,4-dioxygenase, at 2,570,217 to 2,570,795
_RS32060
RR42_RS32065: RR42_RS32065 - protocatechuate 3,4-dioxygenase, beta subunit (EC 1.13.11.3) (from data) , at 2,570,799 to 2,571,524
_RS32065
Position (kb)
2568
2569
2570
2571 Strain fitness (log2 ratio)
-1
0
1
2 at 2568.084 kb on + strand, within RR42_RS32050 at 2568.589 kb on - strand at 2568.589 kb on - strand at 2568.589 kb on - strand at 2568.660 kb on + strand at 2568.661 kb on - strand at 2568.661 kb on - strand at 2568.665 kb on + strand at 2568.804 kb on - strand at 2568.804 kb on - strand at 2569.128 kb on + strand, within RR42_RS32055 at 2569.129 kb on - strand, within RR42_RS32055 at 2569.397 kb on - strand, within RR42_RS32055 at 2569.407 kb on + strand, within RR42_RS32055 at 2569.408 kb on - strand, within RR42_RS32055 at 2569.408 kb on - strand, within RR42_RS32055 at 2570.377 kb on + strand, within RR42_RS32060 at 2570.377 kb on + strand, within RR42_RS32060 at 2570.377 kb on + strand, within RR42_RS32060 at 2570.377 kb on + strand, within RR42_RS32060 at 2570.377 kb on + strand, within RR42_RS32060 at 2570.378 kb on - strand, within RR42_RS32060 at 2570.380 kb on - strand, within RR42_RS32060 at 2570.380 kb on - strand, within RR42_RS32060 at 2570.415 kb on - strand, within RR42_RS32060 at 2570.415 kb on - strand, within RR42_RS32060 at 2570.432 kb on + strand, within RR42_RS32060 at 2570.432 kb on + strand, within RR42_RS32060 at 2570.432 kb on + strand, within RR42_RS32060 at 2570.759 kb on - strand at 2570.789 kb on - strand at 2570.800 kb on + strand at 2570.800 kb on + strand at 2570.801 kb on - strand at 2570.837 kb on + strand at 2570.974 kb on + strand, within RR42_RS32065 at 2570.974 kb on + strand, within RR42_RS32065 at 2570.974 kb on + strand, within RR42_RS32065 at 2570.974 kb on + strand, within RR42_RS32065 at 2570.975 kb on - strand, within RR42_RS32065 at 2570.975 kb on - strand, within RR42_RS32065 at 2570.975 kb on - strand, within RR42_RS32065 at 2570.975 kb on - strand, within RR42_RS32065 at 2571.001 kb on + strand, within RR42_RS32065 at 2571.002 kb on - strand, within RR42_RS32065 at 2571.029 kb on + strand, within RR42_RS32065 at 2571.030 kb on - strand, within RR42_RS32065 at 2571.030 kb on - strand, within RR42_RS32065 at 2571.030 kb on - strand, within RR42_RS32065 at 2571.030 kb on - strand, within RR42_RS32065 at 2571.030 kb on - strand, within RR42_RS32065
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 2,568,084 + RR42_RS32050 0.55 -0.4 2,568,589 - -0.0 2,568,589 - -0.1 2,568,589 - -0.4 2,568,660 + +0.1 2,568,661 - -0.2 2,568,661 - -0.2 2,568,665 + +0.1 2,568,804 - +1.2 2,568,804 - -0.4 2,569,128 + RR42_RS32055 0.28 -0.9 2,569,129 - RR42_RS32055 0.28 -1.0 2,569,397 - RR42_RS32055 0.48 +0.3 2,569,407 + RR42_RS32055 0.49 -1.0 2,569,408 - RR42_RS32055 0.49 -1.3 2,569,408 - RR42_RS32055 0.49 -0.4 2,570,377 + RR42_RS32060 0.28 +0.5 2,570,377 + RR42_RS32060 0.28 -0.6 2,570,377 + RR42_RS32060 0.28 -0.1 2,570,377 + RR42_RS32060 0.28 -0.3 2,570,377 + RR42_RS32060 0.28 -0.9 2,570,378 - RR42_RS32060 0.28 -0.2 2,570,380 - RR42_RS32060 0.28 +0.1 2,570,380 - RR42_RS32060 0.28 -0.1 2,570,415 - RR42_RS32060 0.34 +1.6 2,570,415 - RR42_RS32060 0.34 +0.3 2,570,432 + RR42_RS32060 0.37 +0.0 2,570,432 + RR42_RS32060 0.37 -0.3 2,570,432 + RR42_RS32060 0.37 +0.1 2,570,759 - +0.2 2,570,789 - -0.1 2,570,800 + -0.9 2,570,800 + -0.3 2,570,801 - -0.4 2,570,837 + -0.7 2,570,974 + RR42_RS32065 0.24 -0.0 2,570,974 + RR42_RS32065 0.24 -0.4 2,570,974 + RR42_RS32065 0.24 +1.2 2,570,974 + RR42_RS32065 0.24 -0.5 2,570,975 - RR42_RS32065 0.24 -0.1 2,570,975 - RR42_RS32065 0.24 +0.1 2,570,975 - RR42_RS32065 0.24 -0.1 2,570,975 - RR42_RS32065 0.24 +1.0 2,571,001 + RR42_RS32065 0.28 -0.0 2,571,002 - RR42_RS32065 0.28 +1.0 2,571,029 + RR42_RS32065 0.32 -0.1 2,571,030 - RR42_RS32065 0.32 +1.0 2,571,030 - RR42_RS32065 0.32 +2.1 2,571,030 - RR42_RS32065 0.32 -0.0 2,571,030 - RR42_RS32065 0.32 +0.2 2,571,030 - RR42_RS32065 0.32 +1.1
Or see this region's nucleotide sequence