Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31370 and RR42_RS31375 are separated by 466 nucleotides RR42_RS31375 and RR42_RS31380 are separated by 1 nucleotides RR42_RS31380 and RR42_RS31385 are separated by 80 nucleotides
RR42_RS31370: RR42_RS31370 - acetolactate synthase, at 2,413,569 to 2,415,323
_RS31370
RR42_RS31375: RR42_RS31375 - succinyl-CoA synthetase subunit beta, at 2,415,790 to 2,417,022
_RS31375
RR42_RS31380: RR42_RS31380 - succinate--CoA ligase, at 2,417,024 to 2,417,908
_RS31380
RR42_RS31385: RR42_RS31385 - GntR family transcriptional regulator, at 2,417,989 to 2,418,708
_RS31385
Position (kb)
2415
2416
2417
2418 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2414.825 kb on + strand, within RR42_RS31370 at 2414.826 kb on - strand, within RR42_RS31370 at 2415.073 kb on + strand, within RR42_RS31370 at 2415.151 kb on + strand at 2415.155 kb on + strand at 2415.192 kb on - strand at 2415.570 kb on - strand at 2415.591 kb on + strand at 2415.771 kb on + strand at 2415.805 kb on + strand at 2415.806 kb on - strand at 2415.995 kb on + strand, within RR42_RS31375 at 2416.103 kb on + strand, within RR42_RS31375 at 2416.103 kb on + strand, within RR42_RS31375 at 2416.103 kb on + strand, within RR42_RS31375 at 2416.104 kb on - strand, within RR42_RS31375 at 2416.104 kb on - strand, within RR42_RS31375 at 2416.105 kb on + strand, within RR42_RS31375 at 2416.106 kb on - strand, within RR42_RS31375 at 2416.106 kb on - strand, within RR42_RS31375 at 2416.106 kb on - strand, within RR42_RS31375 at 2416.106 kb on - strand, within RR42_RS31375 at 2416.139 kb on + strand, within RR42_RS31375 at 2416.139 kb on + strand, within RR42_RS31375 at 2416.142 kb on - strand, within RR42_RS31375 at 2416.570 kb on + strand, within RR42_RS31375 at 2416.571 kb on - strand, within RR42_RS31375 at 2416.571 kb on - strand, within RR42_RS31375 at 2416.651 kb on + strand, within RR42_RS31375 at 2416.651 kb on + strand, within RR42_RS31375 at 2416.651 kb on + strand, within RR42_RS31375 at 2416.862 kb on + strand, within RR42_RS31375 at 2416.863 kb on - strand, within RR42_RS31375 at 2416.980 kb on - strand at 2417.006 kb on + strand at 2417.006 kb on + strand at 2417.006 kb on + strand at 2417.006 kb on + strand at 2417.007 kb on - strand at 2417.115 kb on - strand, within RR42_RS31380 at 2417.237 kb on + strand, within RR42_RS31380 at 2417.237 kb on + strand, within RR42_RS31380 at 2417.237 kb on + strand, within RR42_RS31380 at 2417.237 kb on + strand, within RR42_RS31380 at 2417.237 kb on + strand, within RR42_RS31380 at 2417.237 kb on + strand, within RR42_RS31380 at 2417.238 kb on - strand, within RR42_RS31380 at 2417.238 kb on - strand, within RR42_RS31380 at 2417.238 kb on - strand, within RR42_RS31380 at 2417.361 kb on - strand, within RR42_RS31380 at 2417.361 kb on - strand, within RR42_RS31380 at 2417.446 kb on + strand, within RR42_RS31380 at 2417.446 kb on + strand, within RR42_RS31380 at 2417.498 kb on + strand, within RR42_RS31380 at 2417.735 kb on - strand, within RR42_RS31380 at 2417.806 kb on + strand, within RR42_RS31380 at 2417.907 kb on - strand at 2417.939 kb on + strand at 2417.940 kb on - strand at 2417.940 kb on - strand at 2417.956 kb on - strand at 2418.020 kb on + strand at 2418.020 kb on + strand at 2418.021 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 2,414,825 + RR42_RS31370 0.72 -0.5 2,414,826 - RR42_RS31370 0.72 +0.3 2,415,073 + RR42_RS31370 0.86 +0.3 2,415,151 + -0.5 2,415,155 + -0.5 2,415,192 - +0.8 2,415,570 - -0.7 2,415,591 + -0.7 2,415,771 + -1.3 2,415,805 + +0.3 2,415,806 - -0.6 2,415,995 + RR42_RS31375 0.17 -1.5 2,416,103 + RR42_RS31375 0.25 -0.9 2,416,103 + RR42_RS31375 0.25 -0.2 2,416,103 + RR42_RS31375 0.25 -0.1 2,416,104 - RR42_RS31375 0.25 -1.5 2,416,104 - RR42_RS31375 0.25 -0.2 2,416,105 + RR42_RS31375 0.26 -0.9 2,416,106 - RR42_RS31375 0.26 +0.3 2,416,106 - RR42_RS31375 0.26 -1.8 2,416,106 - RR42_RS31375 0.26 -0.6 2,416,106 - RR42_RS31375 0.26 -1.0 2,416,139 + RR42_RS31375 0.28 -0.3 2,416,139 + RR42_RS31375 0.28 +0.3 2,416,142 - RR42_RS31375 0.29 +0.1 2,416,570 + RR42_RS31375 0.63 +0.9 2,416,571 - RR42_RS31375 0.63 -0.4 2,416,571 - RR42_RS31375 0.63 -0.8 2,416,651 + RR42_RS31375 0.70 -0.1 2,416,651 + RR42_RS31375 0.70 -0.4 2,416,651 + RR42_RS31375 0.70 -1.0 2,416,862 + RR42_RS31375 0.87 -2.5 2,416,863 - RR42_RS31375 0.87 -1.0 2,416,980 - +0.2 2,417,006 + +1.4 2,417,006 + -0.6 2,417,006 + -0.8 2,417,006 + -0.4 2,417,007 - +0.0 2,417,115 - RR42_RS31380 0.10 -0.2 2,417,237 + RR42_RS31380 0.24 +0.1 2,417,237 + RR42_RS31380 0.24 +0.2 2,417,237 + RR42_RS31380 0.24 -0.1 2,417,237 + RR42_RS31380 0.24 -0.4 2,417,237 + RR42_RS31380 0.24 -0.2 2,417,237 + RR42_RS31380 0.24 -0.8 2,417,238 - RR42_RS31380 0.24 +0.4 2,417,238 - RR42_RS31380 0.24 -0.5 2,417,238 - RR42_RS31380 0.24 -1.2 2,417,361 - RR42_RS31380 0.38 -0.2 2,417,361 - RR42_RS31380 0.38 +0.7 2,417,446 + RR42_RS31380 0.48 -1.5 2,417,446 + RR42_RS31380 0.48 +1.8 2,417,498 + RR42_RS31380 0.54 +0.5 2,417,735 - RR42_RS31380 0.80 +0.9 2,417,806 + RR42_RS31380 0.88 -1.1 2,417,907 - -0.1 2,417,939 + +0.3 2,417,940 - -0.2 2,417,940 - -0.5 2,417,956 - +0.1 2,418,020 + -0.1 2,418,020 + +0.1 2,418,021 - -0.2
Or see this region's nucleotide sequence