Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS31375

Experiment: L-Isoleucine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS31370 and RR42_RS31375 are separated by 466 nucleotidesRR42_RS31375 and RR42_RS31380 are separated by 1 nucleotidesRR42_RS31380 and RR42_RS31385 are separated by 80 nucleotides RR42_RS31370: RR42_RS31370 - acetolactate synthase, at 2,413,569 to 2,415,323 _RS31370 RR42_RS31375: RR42_RS31375 - succinyl-CoA synthetase subunit beta, at 2,415,790 to 2,417,022 _RS31375 RR42_RS31380: RR42_RS31380 - succinate--CoA ligase, at 2,417,024 to 2,417,908 _RS31380 RR42_RS31385: RR42_RS31385 - GntR family transcriptional regulator, at 2,417,989 to 2,418,708 _RS31385 Position (kb) 2415 2416 2417 2418Strain fitness (log2 ratio) -2 -1 0 1 2at 2414.825 kb on + strand, within RR42_RS31370at 2414.826 kb on - strand, within RR42_RS31370at 2415.073 kb on + strand, within RR42_RS31370at 2415.151 kb on + strandat 2415.155 kb on + strandat 2415.192 kb on - strandat 2415.570 kb on - strandat 2415.591 kb on + strandat 2415.771 kb on + strandat 2415.805 kb on + strandat 2415.806 kb on - strandat 2415.995 kb on + strand, within RR42_RS31375at 2416.103 kb on + strand, within RR42_RS31375at 2416.103 kb on + strand, within RR42_RS31375at 2416.103 kb on + strand, within RR42_RS31375at 2416.104 kb on - strand, within RR42_RS31375at 2416.104 kb on - strand, within RR42_RS31375at 2416.105 kb on + strand, within RR42_RS31375at 2416.106 kb on - strand, within RR42_RS31375at 2416.106 kb on - strand, within RR42_RS31375at 2416.106 kb on - strand, within RR42_RS31375at 2416.106 kb on - strand, within RR42_RS31375at 2416.139 kb on + strand, within RR42_RS31375at 2416.139 kb on + strand, within RR42_RS31375at 2416.142 kb on - strand, within RR42_RS31375at 2416.570 kb on + strand, within RR42_RS31375at 2416.571 kb on - strand, within RR42_RS31375at 2416.571 kb on - strand, within RR42_RS31375at 2416.651 kb on + strand, within RR42_RS31375at 2416.651 kb on + strand, within RR42_RS31375at 2416.651 kb on + strand, within RR42_RS31375at 2416.862 kb on + strand, within RR42_RS31375at 2416.863 kb on - strand, within RR42_RS31375at 2416.980 kb on - strandat 2417.006 kb on + strandat 2417.006 kb on + strandat 2417.006 kb on + strandat 2417.006 kb on + strandat 2417.007 kb on - strandat 2417.115 kb on - strand, within RR42_RS31380at 2417.237 kb on + strand, within RR42_RS31380at 2417.237 kb on + strand, within RR42_RS31380at 2417.237 kb on + strand, within RR42_RS31380at 2417.237 kb on + strand, within RR42_RS31380at 2417.237 kb on + strand, within RR42_RS31380at 2417.237 kb on + strand, within RR42_RS31380at 2417.238 kb on - strand, within RR42_RS31380at 2417.238 kb on - strand, within RR42_RS31380at 2417.238 kb on - strand, within RR42_RS31380at 2417.361 kb on - strand, within RR42_RS31380at 2417.361 kb on - strand, within RR42_RS31380at 2417.446 kb on + strand, within RR42_RS31380at 2417.446 kb on + strand, within RR42_RS31380at 2417.498 kb on + strand, within RR42_RS31380at 2417.735 kb on - strand, within RR42_RS31380at 2417.806 kb on + strand, within RR42_RS31380at 2417.907 kb on - strandat 2417.939 kb on + strandat 2417.940 kb on - strandat 2417.940 kb on - strandat 2417.956 kb on - strandat 2418.020 kb on + strandat 2418.020 kb on + strandat 2418.021 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (N)
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2,414,825 + RR42_RS31370 0.72 -0.5
2,414,826 - RR42_RS31370 0.72 +0.3
2,415,073 + RR42_RS31370 0.86 +0.3
2,415,151 + -0.5
2,415,155 + -0.5
2,415,192 - +0.8
2,415,570 - -0.7
2,415,591 + -0.7
2,415,771 + -1.3
2,415,805 + +0.3
2,415,806 - -0.6
2,415,995 + RR42_RS31375 0.17 -1.5
2,416,103 + RR42_RS31375 0.25 -0.9
2,416,103 + RR42_RS31375 0.25 -0.2
2,416,103 + RR42_RS31375 0.25 -0.1
2,416,104 - RR42_RS31375 0.25 -1.5
2,416,104 - RR42_RS31375 0.25 -0.2
2,416,105 + RR42_RS31375 0.26 -0.9
2,416,106 - RR42_RS31375 0.26 +0.3
2,416,106 - RR42_RS31375 0.26 -1.8
2,416,106 - RR42_RS31375 0.26 -0.6
2,416,106 - RR42_RS31375 0.26 -1.0
2,416,139 + RR42_RS31375 0.28 -0.3
2,416,139 + RR42_RS31375 0.28 +0.3
2,416,142 - RR42_RS31375 0.29 +0.1
2,416,570 + RR42_RS31375 0.63 +0.9
2,416,571 - RR42_RS31375 0.63 -0.4
2,416,571 - RR42_RS31375 0.63 -0.8
2,416,651 + RR42_RS31375 0.70 -0.1
2,416,651 + RR42_RS31375 0.70 -0.4
2,416,651 + RR42_RS31375 0.70 -1.0
2,416,862 + RR42_RS31375 0.87 -2.5
2,416,863 - RR42_RS31375 0.87 -1.0
2,416,980 - +0.2
2,417,006 + +1.4
2,417,006 + -0.6
2,417,006 + -0.8
2,417,006 + -0.4
2,417,007 - +0.0
2,417,115 - RR42_RS31380 0.10 -0.2
2,417,237 + RR42_RS31380 0.24 +0.1
2,417,237 + RR42_RS31380 0.24 +0.2
2,417,237 + RR42_RS31380 0.24 -0.1
2,417,237 + RR42_RS31380 0.24 -0.4
2,417,237 + RR42_RS31380 0.24 -0.2
2,417,237 + RR42_RS31380 0.24 -0.8
2,417,238 - RR42_RS31380 0.24 +0.4
2,417,238 - RR42_RS31380 0.24 -0.5
2,417,238 - RR42_RS31380 0.24 -1.2
2,417,361 - RR42_RS31380 0.38 -0.2
2,417,361 - RR42_RS31380 0.38 +0.7
2,417,446 + RR42_RS31380 0.48 -1.5
2,417,446 + RR42_RS31380 0.48 +1.8
2,417,498 + RR42_RS31380 0.54 +0.5
2,417,735 - RR42_RS31380 0.80 +0.9
2,417,806 + RR42_RS31380 0.88 -1.1
2,417,907 - -0.1
2,417,939 + +0.3
2,417,940 - -0.2
2,417,940 - -0.5
2,417,956 - +0.1
2,418,020 + -0.1
2,418,020 + +0.1
2,418,021 - -0.2

Or see this region's nucleotide sequence