Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31190 and RR42_RS31195 overlap by 4 nucleotides RR42_RS31195 and RR42_RS31200 are separated by 2 nucleotides RR42_RS31200 and RR42_RS31205 overlap by 4 nucleotides RR42_RS31205 and RR42_RS31210 are separated by 38 nucleotides
RR42_RS31190: RR42_RS31190 - 3-ketoacyl-ACP reductase, at 2,375,509 to 2,376,240
_RS31190
RR42_RS31195: RR42_RS31195 - dehydratase, at 2,376,237 to 2,376,707
_RS31195
RR42_RS31200: RR42_RS31200 - 3-oxoacyl-ACP synthase, at 2,376,710 to 2,377,891
_RS31200
RR42_RS31205: RR42_RS31205 - phospho-N-acetylmuramoyl-pentapeptide transferase, at 2,377,888 to 2,378,496
_RS31205
RR42_RS31210: RR42_RS31210 - FAD-dependent oxidoreductase, at 2,378,535 to 2,379,764
_RS31210
Position (kb)
2376
2377
2378 Strain fitness (log2 ratio)
-1
0
1
2 at 2375.780 kb on + strand, within RR42_RS31190 at 2375.780 kb on + strand, within RR42_RS31190 at 2375.780 kb on + strand, within RR42_RS31190 at 2375.780 kb on + strand, within RR42_RS31190 at 2375.780 kb on + strand, within RR42_RS31190 at 2375.781 kb on - strand, within RR42_RS31190 at 2375.781 kb on - strand, within RR42_RS31190 at 2375.781 kb on - strand, within RR42_RS31190 at 2375.781 kb on - strand, within RR42_RS31190 at 2375.891 kb on + strand, within RR42_RS31190 at 2375.892 kb on - strand, within RR42_RS31190 at 2375.994 kb on - strand, within RR42_RS31190 at 2375.997 kb on - strand, within RR42_RS31190 at 2376.159 kb on - strand, within RR42_RS31190 at 2376.384 kb on + strand, within RR42_RS31195 at 2376.384 kb on + strand, within RR42_RS31195 at 2376.385 kb on - strand, within RR42_RS31195 at 2376.385 kb on - strand, within RR42_RS31195 at 2376.431 kb on - strand, within RR42_RS31195 at 2376.523 kb on + strand, within RR42_RS31195 at 2376.523 kb on + strand, within RR42_RS31195 at 2376.524 kb on - strand, within RR42_RS31195 at 2376.524 kb on - strand, within RR42_RS31195 at 2376.725 kb on + strand at 2376.726 kb on - strand at 2376.726 kb on - strand at 2377.056 kb on + strand, within RR42_RS31200 at 2377.057 kb on - strand, within RR42_RS31200 at 2377.437 kb on + strand, within RR42_RS31200 at 2377.438 kb on - strand, within RR42_RS31200 at 2377.582 kb on + strand, within RR42_RS31200 at 2377.671 kb on + strand, within RR42_RS31200 at 2377.773 kb on + strand at 2377.774 kb on - strand at 2377.879 kb on + strand at 2377.941 kb on - strand at 2378.121 kb on + strand, within RR42_RS31205 at 2378.122 kb on - strand, within RR42_RS31205 at 2378.300 kb on + strand, within RR42_RS31205 at 2378.300 kb on + strand, within RR42_RS31205 at 2378.301 kb on - strand, within RR42_RS31205 at 2378.301 kb on - strand, within RR42_RS31205 at 2378.614 kb on + strand at 2378.614 kb on + strand at 2378.615 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 2,375,780 + RR42_RS31190 0.37 +0.4 2,375,780 + RR42_RS31190 0.37 +0.1 2,375,780 + RR42_RS31190 0.37 +0.6 2,375,780 + RR42_RS31190 0.37 -0.7 2,375,780 + RR42_RS31190 0.37 +0.1 2,375,781 - RR42_RS31190 0.37 +0.5 2,375,781 - RR42_RS31190 0.37 +0.0 2,375,781 - RR42_RS31190 0.37 -1.7 2,375,781 - RR42_RS31190 0.37 +0.2 2,375,891 + RR42_RS31190 0.52 +2.6 2,375,892 - RR42_RS31190 0.52 +0.1 2,375,994 - RR42_RS31190 0.66 -0.2 2,375,997 - RR42_RS31190 0.67 -0.0 2,376,159 - RR42_RS31190 0.89 +0.1 2,376,384 + RR42_RS31195 0.31 +0.3 2,376,384 + RR42_RS31195 0.31 +0.8 2,376,385 - RR42_RS31195 0.31 +0.6 2,376,385 - RR42_RS31195 0.31 +1.3 2,376,431 - RR42_RS31195 0.41 +0.5 2,376,523 + RR42_RS31195 0.61 -0.8 2,376,523 + RR42_RS31195 0.61 +0.1 2,376,524 - RR42_RS31195 0.61 -0.6 2,376,524 - RR42_RS31195 0.61 +0.4 2,376,725 + +0.3 2,376,726 - -0.4 2,376,726 - -0.5 2,377,056 + RR42_RS31200 0.29 -0.4 2,377,057 - RR42_RS31200 0.29 +0.8 2,377,437 + RR42_RS31200 0.62 +2.2 2,377,438 - RR42_RS31200 0.62 +0.1 2,377,582 + RR42_RS31200 0.74 -0.4 2,377,671 + RR42_RS31200 0.81 +1.0 2,377,773 + -0.3 2,377,774 - +0.6 2,377,879 + +0.6 2,377,941 - +0.2 2,378,121 + RR42_RS31205 0.38 +0.9 2,378,122 - RR42_RS31205 0.38 +0.8 2,378,300 + RR42_RS31205 0.68 +0.1 2,378,300 + RR42_RS31205 0.68 -1.3 2,378,301 - RR42_RS31205 0.68 -0.6 2,378,301 - RR42_RS31205 0.68 -1.2 2,378,614 + +0.1 2,378,614 + +0.7 2,378,615 - -0.1
Or see this region's nucleotide sequence