Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS25720

Experiment: L-Isoleucine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS25710 and RR42_RS25715 are separated by 217 nucleotidesRR42_RS25715 and RR42_RS25720 are separated by 291 nucleotidesRR42_RS25720 and RR42_RS25725 are separated by 21 nucleotidesRR42_RS25725 and RR42_RS25730 are separated by 43 nucleotides RR42_RS25710: RR42_RS25710 - Clp protease, at 1,110,612 to 1,110,956 _RS25710 RR42_RS25715: RR42_RS25715 - hypothetical protein, at 1,111,174 to 1,111,404 _RS25715 RR42_RS25720: RR42_RS25720 - hypothetical protein, at 1,111,696 to 1,112,091 _RS25720 RR42_RS25725: RR42_RS25725 - hypothetical protein, at 1,112,113 to 1,112,778 _RS25725 RR42_RS25730: RR42_RS25730 - hypothetical protein, at 1,112,822 to 1,113,469 _RS25730 Position (kb) 1111 1112 1113Strain fitness (log2 ratio) -2 -1 0 1 2at 1110.714 kb on + strand, within RR42_RS25710at 1110.714 kb on + strand, within RR42_RS25710at 1110.714 kb on + strand, within RR42_RS25710at 1110.714 kb on + strand, within RR42_RS25710at 1110.715 kb on - strandat 1110.740 kb on + strand, within RR42_RS25710at 1110.794 kb on + strand, within RR42_RS25710at 1110.794 kb on + strand, within RR42_RS25710at 1111.261 kb on - strandat 1111.261 kb on - strand, within RR42_RS25715at 1111.330 kb on + strand, within RR42_RS25715at 1111.330 kb on + strand, within RR42_RS25715at 1111.331 kb on - strand, within RR42_RS25715at 1111.331 kb on - strand, within RR42_RS25715at 1111.348 kb on + strand, within RR42_RS25715at 1111.433 kb on - strandat 1111.436 kb on - strandat 1111.506 kb on + strandat 1111.506 kb on + strandat 1111.526 kb on - strandat 1111.526 kb on - strandat 1111.555 kb on - strandat 1111.555 kb on - strandat 1111.555 kb on - strandat 1111.669 kb on + strandat 1111.669 kb on + strandat 1111.669 kb on + strandat 1111.669 kb on + strandat 1111.669 kb on + strandat 1111.823 kb on - strand, within RR42_RS25720at 1111.823 kb on - strand, within RR42_RS25720at 1111.961 kb on - strand, within RR42_RS25720at 1111.961 kb on - strand, within RR42_RS25720at 1111.961 kb on - strand, within RR42_RS25720at 1111.997 kb on - strand, within RR42_RS25720at 1111.997 kb on - strand, within RR42_RS25720at 1112.059 kb on - strandat 1112.225 kb on + strand, within RR42_RS25725at 1112.225 kb on + strand, within RR42_RS25725at 1112.246 kb on + strand, within RR42_RS25725at 1112.247 kb on - strand, within RR42_RS25725at 1112.348 kb on - strand, within RR42_RS25725at 1112.489 kb on + strand, within RR42_RS25725at 1112.490 kb on - strand, within RR42_RS25725at 1112.611 kb on + strand, within RR42_RS25725at 1112.612 kb on - strand, within RR42_RS25725at 1112.823 kb on + strandat 1112.823 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (N)
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1,110,714 + RR42_RS25710 0.30 -0.2
1,110,714 + RR42_RS25710 0.30 +0.4
1,110,714 + RR42_RS25710 0.30 -1.2
1,110,714 + RR42_RS25710 0.30 -1.1
1,110,715 - +2.5
1,110,740 + RR42_RS25710 0.37 +0.6
1,110,794 + RR42_RS25710 0.53 -0.2
1,110,794 + RR42_RS25710 0.53 +0.2
1,111,261 - +1.5
1,111,261 - RR42_RS25715 0.38 -0.6
1,111,330 + RR42_RS25715 0.68 +0.0
1,111,330 + RR42_RS25715 0.68 -0.4
1,111,331 - RR42_RS25715 0.68 -1.1
1,111,331 - RR42_RS25715 0.68 -0.5
1,111,348 + RR42_RS25715 0.75 +0.5
1,111,433 - -0.9
1,111,436 - -0.3
1,111,506 + +0.9
1,111,506 + -0.1
1,111,526 - -0.3
1,111,526 - +0.6
1,111,555 - +1.1
1,111,555 - +1.5
1,111,555 - -0.2
1,111,669 + -0.5
1,111,669 + -1.5
1,111,669 + -1.0
1,111,669 + -0.4
1,111,669 + +0.6
1,111,823 - RR42_RS25720 0.32 +0.4
1,111,823 - RR42_RS25720 0.32 +0.2
1,111,961 - RR42_RS25720 0.67 +1.1
1,111,961 - RR42_RS25720 0.67 +0.1
1,111,961 - RR42_RS25720 0.67 -0.6
1,111,997 - RR42_RS25720 0.76 -0.1
1,111,997 - RR42_RS25720 0.76 +0.6
1,112,059 - -0.3
1,112,225 + RR42_RS25725 0.17 +0.4
1,112,225 + RR42_RS25725 0.17 +0.7
1,112,246 + RR42_RS25725 0.20 -0.4
1,112,247 - RR42_RS25725 0.20 -0.9
1,112,348 - RR42_RS25725 0.35 -0.8
1,112,489 + RR42_RS25725 0.56 +0.1
1,112,490 - RR42_RS25725 0.57 -1.8
1,112,611 + RR42_RS25725 0.75 +0.1
1,112,612 - RR42_RS25725 0.75 -0.6
1,112,823 + +0.4
1,112,823 + -0.7

Or see this region's nucleotide sequence