Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS25710 and RR42_RS25715 are separated by 217 nucleotides RR42_RS25715 and RR42_RS25720 are separated by 291 nucleotides RR42_RS25720 and RR42_RS25725 are separated by 21 nucleotides RR42_RS25725 and RR42_RS25730 are separated by 43 nucleotides
RR42_RS25710: RR42_RS25710 - Clp protease, at 1,110,612 to 1,110,956
_RS25710
RR42_RS25715: RR42_RS25715 - hypothetical protein, at 1,111,174 to 1,111,404
_RS25715
RR42_RS25720: RR42_RS25720 - hypothetical protein, at 1,111,696 to 1,112,091
_RS25720
RR42_RS25725: RR42_RS25725 - hypothetical protein, at 1,112,113 to 1,112,778
_RS25725
RR42_RS25730: RR42_RS25730 - hypothetical protein, at 1,112,822 to 1,113,469
_RS25730
Position (kb)
1111
1112
1113 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1110.714 kb on + strand, within RR42_RS25710 at 1110.714 kb on + strand, within RR42_RS25710 at 1110.714 kb on + strand, within RR42_RS25710 at 1110.714 kb on + strand, within RR42_RS25710 at 1110.715 kb on - strand at 1110.740 kb on + strand, within RR42_RS25710 at 1110.794 kb on + strand, within RR42_RS25710 at 1110.794 kb on + strand, within RR42_RS25710 at 1111.261 kb on - strand at 1111.261 kb on - strand, within RR42_RS25715 at 1111.330 kb on + strand, within RR42_RS25715 at 1111.330 kb on + strand, within RR42_RS25715 at 1111.331 kb on - strand, within RR42_RS25715 at 1111.331 kb on - strand, within RR42_RS25715 at 1111.348 kb on + strand, within RR42_RS25715 at 1111.433 kb on - strand at 1111.436 kb on - strand at 1111.506 kb on + strand at 1111.506 kb on + strand at 1111.526 kb on - strand at 1111.526 kb on - strand at 1111.555 kb on - strand at 1111.555 kb on - strand at 1111.555 kb on - strand at 1111.669 kb on + strand at 1111.669 kb on + strand at 1111.669 kb on + strand at 1111.669 kb on + strand at 1111.669 kb on + strand at 1111.823 kb on - strand, within RR42_RS25720 at 1111.823 kb on - strand, within RR42_RS25720 at 1111.961 kb on - strand, within RR42_RS25720 at 1111.961 kb on - strand, within RR42_RS25720 at 1111.961 kb on - strand, within RR42_RS25720 at 1111.997 kb on - strand, within RR42_RS25720 at 1111.997 kb on - strand, within RR42_RS25720 at 1112.059 kb on - strand at 1112.225 kb on + strand, within RR42_RS25725 at 1112.225 kb on + strand, within RR42_RS25725 at 1112.246 kb on + strand, within RR42_RS25725 at 1112.247 kb on - strand, within RR42_RS25725 at 1112.348 kb on - strand, within RR42_RS25725 at 1112.489 kb on + strand, within RR42_RS25725 at 1112.490 kb on - strand, within RR42_RS25725 at 1112.611 kb on + strand, within RR42_RS25725 at 1112.612 kb on - strand, within RR42_RS25725 at 1112.823 kb on + strand at 1112.823 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,110,714 + RR42_RS25710 0.30 -0.2 1,110,714 + RR42_RS25710 0.30 +0.4 1,110,714 + RR42_RS25710 0.30 -1.2 1,110,714 + RR42_RS25710 0.30 -1.1 1,110,715 - +2.5 1,110,740 + RR42_RS25710 0.37 +0.6 1,110,794 + RR42_RS25710 0.53 -0.2 1,110,794 + RR42_RS25710 0.53 +0.2 1,111,261 - +1.5 1,111,261 - RR42_RS25715 0.38 -0.6 1,111,330 + RR42_RS25715 0.68 +0.0 1,111,330 + RR42_RS25715 0.68 -0.4 1,111,331 - RR42_RS25715 0.68 -1.1 1,111,331 - RR42_RS25715 0.68 -0.5 1,111,348 + RR42_RS25715 0.75 +0.5 1,111,433 - -0.9 1,111,436 - -0.3 1,111,506 + +0.9 1,111,506 + -0.1 1,111,526 - -0.3 1,111,526 - +0.6 1,111,555 - +1.1 1,111,555 - +1.5 1,111,555 - -0.2 1,111,669 + -0.5 1,111,669 + -1.5 1,111,669 + -1.0 1,111,669 + -0.4 1,111,669 + +0.6 1,111,823 - RR42_RS25720 0.32 +0.4 1,111,823 - RR42_RS25720 0.32 +0.2 1,111,961 - RR42_RS25720 0.67 +1.1 1,111,961 - RR42_RS25720 0.67 +0.1 1,111,961 - RR42_RS25720 0.67 -0.6 1,111,997 - RR42_RS25720 0.76 -0.1 1,111,997 - RR42_RS25720 0.76 +0.6 1,112,059 - -0.3 1,112,225 + RR42_RS25725 0.17 +0.4 1,112,225 + RR42_RS25725 0.17 +0.7 1,112,246 + RR42_RS25725 0.20 -0.4 1,112,247 - RR42_RS25725 0.20 -0.9 1,112,348 - RR42_RS25725 0.35 -0.8 1,112,489 + RR42_RS25725 0.56 +0.1 1,112,490 - RR42_RS25725 0.57 -1.8 1,112,611 + RR42_RS25725 0.75 +0.1 1,112,612 - RR42_RS25725 0.75 -0.6 1,112,823 + +0.4 1,112,823 + -0.7
Or see this region's nucleotide sequence