Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS17235

Experiment: L-Isoleucine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS17225 and RR42_RS17230 are separated by 115 nucleotidesRR42_RS17230 and RR42_RS17235 are separated by 1 nucleotidesRR42_RS17235 and RR42_RS17240 overlap by 1 nucleotidesRR42_RS17240 and RR42_RS17245 are separated by 82 nucleotides RR42_RS17225: RR42_RS17225 - DnaA regulatory inactivator Hda, at 3,729,546 to 3,730,253 _RS17225 RR42_RS17230: RR42_RS17230 - phosphoserine phosphatase, at 3,730,369 to 3,731,043 _RS17230 RR42_RS17235: RR42_RS17235 - poly(A) polymerase, at 3,731,045 to 3,732,658 _RS17235 RR42_RS17240: RR42_RS17240 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase, at 3,732,658 to 3,733,176 _RS17240 RR42_RS17245: RR42_RS17245 - deoxynucleoside kinase, at 3,733,259 to 3,733,900 _RS17245 Position (kb) 3731 3732 3733Strain fitness (log2 ratio) -2 -1 0 1 2at 3730.243 kb on + strandat 3730.243 kb on + strandat 3730.327 kb on + strandat 3730.327 kb on + strandat 3730.350 kb on + strandat 3730.351 kb on - strandat 3730.351 kb on - strandat 3730.356 kb on - strandat 3730.474 kb on + strand, within RR42_RS17230at 3730.507 kb on + strand, within RR42_RS17230at 3730.507 kb on + strand, within RR42_RS17230at 3730.508 kb on - strand, within RR42_RS17230at 3730.508 kb on - strand, within RR42_RS17230at 3730.660 kb on + strand, within RR42_RS17230at 3730.710 kb on + strand, within RR42_RS17230at 3730.711 kb on - strand, within RR42_RS17230at 3730.850 kb on + strand, within RR42_RS17230at 3730.850 kb on + strand, within RR42_RS17230at 3730.850 kb on + strand, within RR42_RS17230at 3730.851 kb on - strand, within RR42_RS17230at 3730.918 kb on + strand, within RR42_RS17230at 3730.919 kb on - strand, within RR42_RS17230at 3730.919 kb on - strand, within RR42_RS17230at 3730.919 kb on - strand, within RR42_RS17230at 3731.074 kb on - strandat 3731.074 kb on - strandat 3731.129 kb on - strandat 3731.138 kb on - strandat 3731.225 kb on + strand, within RR42_RS17235at 3731.387 kb on + strand, within RR42_RS17235at 3731.388 kb on - strand, within RR42_RS17235at 3731.388 kb on - strand, within RR42_RS17235at 3731.388 kb on - strand, within RR42_RS17235at 3731.388 kb on - strand, within RR42_RS17235at 3731.606 kb on + strand, within RR42_RS17235at 3731.607 kb on - strand, within RR42_RS17235at 3731.607 kb on - strand, within RR42_RS17235at 3731.607 kb on - strand, within RR42_RS17235at 3731.607 kb on - strand, within RR42_RS17235at 3731.610 kb on - strand, within RR42_RS17235at 3731.633 kb on + strand, within RR42_RS17235at 3731.639 kb on + strand, within RR42_RS17235at 3731.640 kb on - strand, within RR42_RS17235at 3732.057 kb on + strand, within RR42_RS17235at 3732.057 kb on + strand, within RR42_RS17235at 3732.058 kb on - strand, within RR42_RS17235at 3732.303 kb on - strand, within RR42_RS17235at 3732.303 kb on - strand, within RR42_RS17235at 3732.580 kb on + strandat 3732.633 kb on + strandat 3733.222 kb on + strandat 3733.222 kb on + strandat 3733.222 kb on + strandat 3733.223 kb on - strandat 3733.223 kb on - strandat 3733.223 kb on - strandat 3733.223 kb on - strandat 3733.223 kb on - strandat 3733.223 kb on - strandat 3733.225 kb on + strandat 3733.225 kb on + strandat 3733.225 kb on + strandat 3733.225 kb on + strandat 3733.225 kb on + strandat 3733.225 kb on + strandat 3733.225 kb on + strandat 3733.225 kb on + strandat 3733.226 kb on - strandat 3733.226 kb on - strandat 3733.226 kb on - strandat 3733.226 kb on - strandat 3733.226 kb on - strandat 3733.226 kb on - strandat 3733.226 kb on - strandat 3733.226 kb on - strandat 3733.229 kb on + strandat 3733.229 kb on + strandat 3733.229 kb on + strandat 3733.229 kb on + strandat 3733.229 kb on + strandat 3733.229 kb on + strandat 3733.229 kb on + strandat 3733.229 kb on + strandat 3733.230 kb on - strandat 3733.230 kb on - strandat 3733.230 kb on - strandat 3733.230 kb on - strandat 3733.230 kb on - strandat 3733.230 kb on - strandat 3733.397 kb on + strand, within RR42_RS17245at 3733.400 kb on + strand, within RR42_RS17245at 3733.541 kb on + strand, within RR42_RS17245at 3733.581 kb on - strand, within RR42_RS17245at 3733.581 kb on - strand, within RR42_RS17245at 3733.624 kb on - strand, within RR42_RS17245at 3733.638 kb on + strand, within RR42_RS17245at 3733.638 kb on + strand, within RR42_RS17245

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (N)
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3,730,243 + -0.8
3,730,243 + -0.5
3,730,327 + -0.6
3,730,327 + -0.1
3,730,350 + +0.5
3,730,351 - -0.2
3,730,351 - +0.3
3,730,356 - +1.7
3,730,474 + RR42_RS17230 0.16 +0.2
3,730,507 + RR42_RS17230 0.20 +1.4
3,730,507 + RR42_RS17230 0.20 -2.4
3,730,508 - RR42_RS17230 0.21 +0.7
3,730,508 - RR42_RS17230 0.21 +0.3
3,730,660 + RR42_RS17230 0.43 +0.2
3,730,710 + RR42_RS17230 0.51 +0.5
3,730,711 - RR42_RS17230 0.51 +1.7
3,730,850 + RR42_RS17230 0.71 +0.4
3,730,850 + RR42_RS17230 0.71 -0.0
3,730,850 + RR42_RS17230 0.71 -0.8
3,730,851 - RR42_RS17230 0.71 +1.2
3,730,918 + RR42_RS17230 0.81 +0.0
3,730,919 - RR42_RS17230 0.81 +0.9
3,730,919 - RR42_RS17230 0.81 +1.2
3,730,919 - RR42_RS17230 0.81 +1.2
3,731,074 - +1.7
3,731,074 - +1.9
3,731,129 - +2.4
3,731,138 - +1.3
3,731,225 + RR42_RS17235 0.11 +0.4
3,731,387 + RR42_RS17235 0.21 +0.1
3,731,388 - RR42_RS17235 0.21 +0.4
3,731,388 - RR42_RS17235 0.21 +1.2
3,731,388 - RR42_RS17235 0.21 +0.6
3,731,388 - RR42_RS17235 0.21 +1.3
3,731,606 + RR42_RS17235 0.35 +1.4
3,731,607 - RR42_RS17235 0.35 +1.2
3,731,607 - RR42_RS17235 0.35 +0.7
3,731,607 - RR42_RS17235 0.35 +0.9
3,731,607 - RR42_RS17235 0.35 +1.6
3,731,610 - RR42_RS17235 0.35 +1.3
3,731,633 + RR42_RS17235 0.36 +0.9
3,731,639 + RR42_RS17235 0.37 +0.8
3,731,640 - RR42_RS17235 0.37 -0.4
3,732,057 + RR42_RS17235 0.63 +0.9
3,732,057 + RR42_RS17235 0.63 +1.0
3,732,058 - RR42_RS17235 0.63 +2.5
3,732,303 - RR42_RS17235 0.78 +0.2
3,732,303 - RR42_RS17235 0.78 +1.3
3,732,580 + -0.2
3,732,633 + -0.2
3,733,222 + -0.3
3,733,222 + -1.5
3,733,222 + -0.0
3,733,223 - +0.5
3,733,223 - +1.0
3,733,223 - +0.1
3,733,223 - -0.0
3,733,223 - +0.3
3,733,223 - +1.3
3,733,225 + -0.3
3,733,225 + -0.1
3,733,225 + +0.4
3,733,225 + -0.4
3,733,225 + -0.7
3,733,225 + -0.6
3,733,225 + -0.1
3,733,225 + +0.3
3,733,226 - +0.8
3,733,226 - +0.9
3,733,226 - +1.2
3,733,226 - +0.2
3,733,226 - -0.2
3,733,226 - +0.1
3,733,226 - +0.2
3,733,226 - -0.7
3,733,229 + +0.0
3,733,229 + +0.3
3,733,229 + +0.2
3,733,229 + +0.1
3,733,229 + -1.4
3,733,229 + +0.7
3,733,229 + -0.3
3,733,229 + +0.2
3,733,230 - +1.2
3,733,230 - +0.9
3,733,230 - +0.0
3,733,230 - -0.1
3,733,230 - +2.2
3,733,230 - +0.2
3,733,397 + RR42_RS17245 0.21 +0.3
3,733,400 + RR42_RS17245 0.22 -0.2
3,733,541 + RR42_RS17245 0.44 +0.2
3,733,581 - RR42_RS17245 0.50 +1.1
3,733,581 - RR42_RS17245 0.50 -0.1
3,733,624 - RR42_RS17245 0.57 -0.4
3,733,638 + RR42_RS17245 0.59 +0.2
3,733,638 + RR42_RS17245 0.59 +1.2

Or see this region's nucleotide sequence