Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS16640 and RR42_RS16645 are separated by 129 nucleotides RR42_RS16645 and RR42_RS16650 are separated by 54 nucleotides RR42_RS16650 and RR42_RS16655 are separated by 105 nucleotides
RR42_RS16640: RR42_RS16640 - LysR family transcriptional regulator, at 3,606,599 to 3,607,483
_RS16640
RR42_RS16645: RR42_RS16645 - n-acetyl-gamma-glutamyl-phosphate reductase, at 3,607,613 to 3,608,542
_RS16645
RR42_RS16650: RR42_RS16650 - hypothetical protein, at 3,608,597 to 3,609,100
_RS16650
RR42_RS16655: RR42_RS16655 - glutamine cyclotransferase, at 3,609,206 to 3,609,892
_RS16655
Position (kb)
3607
3608
3609 Strain fitness (log2 ratio)
-2
-1
0
1 at 3606.633 kb on - strand at 3606.811 kb on - strand, within RR42_RS16640 at 3607.231 kb on + strand, within RR42_RS16640 at 3607.529 kb on - strand at 3607.540 kb on + strand at 3607.563 kb on - strand at 3607.583 kb on - strand at 3607.604 kb on - strand at 3607.612 kb on + strand at 3607.704 kb on + strand at 3607.705 kb on - strand at 3607.889 kb on + strand, within RR42_RS16645 at 3607.965 kb on - strand, within RR42_RS16645 at 3607.965 kb on - strand, within RR42_RS16645 at 3607.965 kb on - strand, within RR42_RS16645 at 3608.130 kb on + strand, within RR42_RS16645 at 3608.130 kb on + strand, within RR42_RS16645 at 3608.131 kb on - strand, within RR42_RS16645 at 3608.131 kb on - strand, within RR42_RS16645 at 3608.132 kb on + strand, within RR42_RS16645 at 3608.132 kb on + strand, within RR42_RS16645 at 3608.298 kb on + strand, within RR42_RS16645 at 3608.340 kb on + strand, within RR42_RS16645 at 3608.340 kb on + strand, within RR42_RS16645 at 3608.341 kb on - strand, within RR42_RS16645 at 3608.341 kb on - strand, within RR42_RS16645 at 3608.341 kb on - strand, within RR42_RS16645 at 3608.540 kb on + strand at 3608.548 kb on - strand at 3608.548 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 3,606,633 - +0.5 3,606,811 - RR42_RS16640 0.24 +0.7 3,607,231 + RR42_RS16640 0.71 +1.3 3,607,529 - +0.7 3,607,540 + +0.2 3,607,563 - +0.3 3,607,583 - -2.4 3,607,604 - -0.4 3,607,612 + -0.8 3,607,704 + -0.3 3,607,705 - -0.4 3,607,889 + RR42_RS16645 0.30 -0.9 3,607,965 - RR42_RS16645 0.38 -0.2 3,607,965 - RR42_RS16645 0.38 -1.6 3,607,965 - RR42_RS16645 0.38 +0.1 3,608,130 + RR42_RS16645 0.56 +0.3 3,608,130 + RR42_RS16645 0.56 +0.3 3,608,131 - RR42_RS16645 0.56 -0.3 3,608,131 - RR42_RS16645 0.56 -0.0 3,608,132 + RR42_RS16645 0.56 +0.7 3,608,132 + RR42_RS16645 0.56 -0.8 3,608,298 + RR42_RS16645 0.74 -0.0 3,608,340 + RR42_RS16645 0.78 +0.3 3,608,340 + RR42_RS16645 0.78 +0.5 3,608,341 - RR42_RS16645 0.78 +0.1 3,608,341 - RR42_RS16645 0.78 +0.1 3,608,341 - RR42_RS16645 0.78 -0.3 3,608,540 + +0.0 3,608,548 - -0.2 3,608,548 - -0.2
Or see this region's nucleotide sequence