Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS12010 and RR42_RS12015 are separated by 74 nucleotides RR42_RS12015 and RR42_RS12020 are separated by 125 nucleotides RR42_RS12020 and RR42_RS12025 are separated by 76 nucleotides RR42_RS12025 and RR42_RS12030 are separated by 82 nucleotides
RR42_RS12010: RR42_RS12010 - ABC transporter, at 2,574,309 to 2,576,249
_RS12010
RR42_RS12015: RR42_RS12015 - dihydrolipoamide acyltransferase, at 2,576,324 to 2,576,809
_RS12015
RR42_RS12020: RR42_RS12020 - GCN5 family acetyltransferase, at 2,576,935 to 2,577,471
_RS12020
RR42_RS12025: RR42_RS12025 - hypothetical protein, at 2,577,548 to 2,578,123
_RS12025
RR42_RS12030: RR42_RS12030 - DNA repair protein RadA, at 2,578,206 to 2,579,567
_RS12030
Position (kb)
2576
2577
2578 Strain fitness (log2 ratio)
-2
-1
0
1 at 2576.124 kb on + strand at 2576.124 kb on + strand at 2576.124 kb on + strand at 2576.125 kb on - strand at 2576.125 kb on - strand at 2576.331 kb on + strand at 2576.332 kb on - strand at 2576.460 kb on + strand, within RR42_RS12015 at 2576.460 kb on + strand, within RR42_RS12015 at 2576.461 kb on - strand, within RR42_RS12015 at 2576.818 kb on - strand at 2576.856 kb on - strand at 2576.856 kb on - strand at 2576.867 kb on + strand at 2576.867 kb on + strand at 2576.867 kb on + strand at 2576.868 kb on - strand at 2576.868 kb on - strand at 2577.158 kb on + strand, within RR42_RS12020 at 2577.158 kb on + strand, within RR42_RS12020 at 2577.158 kb on + strand, within RR42_RS12020 at 2577.588 kb on + strand at 2577.589 kb on - strand at 2577.841 kb on + strand, within RR42_RS12025 at 2577.851 kb on + strand, within RR42_RS12025 at 2577.851 kb on + strand, within RR42_RS12025 at 2577.852 kb on - strand, within RR42_RS12025 at 2577.903 kb on + strand, within RR42_RS12025 at 2577.903 kb on + strand, within RR42_RS12025 at 2577.903 kb on + strand, within RR42_RS12025 at 2577.903 kb on + strand, within RR42_RS12025 at 2577.903 kb on + strand, within RR42_RS12025 at 2577.903 kb on + strand, within RR42_RS12025 at 2577.903 kb on + strand, within RR42_RS12025 at 2577.904 kb on - strand, within RR42_RS12025 at 2577.904 kb on - strand, within RR42_RS12025 at 2577.904 kb on - strand, within RR42_RS12025 at 2577.904 kb on - strand, within RR42_RS12025 at 2577.961 kb on - strand, within RR42_RS12025 at 2577.961 kb on - strand, within RR42_RS12025 at 2577.987 kb on + strand, within RR42_RS12025 at 2577.988 kb on - strand, within RR42_RS12025 at 2577.988 kb on - strand, within RR42_RS12025 at 2578.162 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 2,576,124 + +1.0 2,576,124 + -1.5 2,576,124 + +0.6 2,576,125 - -0.5 2,576,125 - -1.5 2,576,331 + +0.4 2,576,332 - -2.2 2,576,460 + RR42_RS12015 0.28 -0.4 2,576,460 + RR42_RS12015 0.28 -1.2 2,576,461 - RR42_RS12015 0.28 -1.9 2,576,818 - -1.9 2,576,856 - +0.3 2,576,856 - -1.2 2,576,867 + -1.1 2,576,867 + -0.5 2,576,867 + -2.0 2,576,868 - -0.5 2,576,868 - +1.1 2,577,158 + RR42_RS12020 0.42 +0.8 2,577,158 + RR42_RS12020 0.42 +0.5 2,577,158 + RR42_RS12020 0.42 +1.1 2,577,588 + -0.8 2,577,589 - -0.7 2,577,841 + RR42_RS12025 0.51 +0.5 2,577,851 + RR42_RS12025 0.53 -0.2 2,577,851 + RR42_RS12025 0.53 -0.4 2,577,852 - RR42_RS12025 0.53 +0.2 2,577,903 + RR42_RS12025 0.62 -0.3 2,577,903 + RR42_RS12025 0.62 -0.4 2,577,903 + RR42_RS12025 0.62 -1.0 2,577,903 + RR42_RS12025 0.62 +0.2 2,577,903 + RR42_RS12025 0.62 +0.5 2,577,903 + RR42_RS12025 0.62 +0.8 2,577,903 + RR42_RS12025 0.62 -0.5 2,577,904 - RR42_RS12025 0.62 +0.8 2,577,904 - RR42_RS12025 0.62 +0.2 2,577,904 - RR42_RS12025 0.62 -0.2 2,577,904 - RR42_RS12025 0.62 -0.6 2,577,961 - RR42_RS12025 0.72 -0.1 2,577,961 - RR42_RS12025 0.72 -1.8 2,577,987 + RR42_RS12025 0.76 -0.4 2,577,988 - RR42_RS12025 0.76 -0.0 2,577,988 - RR42_RS12025 0.76 -0.5 2,578,162 - +0.2
Or see this region's nucleotide sequence