Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS11770 and RR42_RS11775 are separated by 238 nucleotides RR42_RS11775 and RR42_RS11780 are separated by 74 nucleotides RR42_RS11780 and RR42_RS11785 are separated by 73 nucleotides RR42_RS11785 and RR42_RS11790 are separated by 154 nucleotides
RR42_RS11770: RR42_RS11770 - cyclase, at 2,516,998 to 2,517,435
_RS11770
RR42_RS11775: RR42_RS11775 - single-stranded DNA-binding protein, at 2,517,674 to 2,518,126
_RS11775
RR42_RS11780: RR42_RS11780 - stomatin 2, at 2,518,201 to 2,519,133
_RS11780
RR42_RS11785: RR42_RS11785 - NfeD-like family protein 1, at 2,519,207 to 2,519,632
_RS11785
RR42_RS11790: RR42_RS11790 - phosphoenolpyruvate synthase, at 2,519,787 to 2,522,171
_RS11790
Position (kb)
2518
2519
2520 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2517.383 kb on + strand, within RR42_RS11770 at 2517.383 kb on + strand, within RR42_RS11770 at 2517.421 kb on - strand at 2517.421 kb on - strand at 2517.438 kb on - strand at 2517.438 kb on - strand at 2517.438 kb on - strand at 2517.438 kb on - strand at 2517.438 kb on - strand at 2517.643 kb on + strand at 2518.124 kb on + strand at 2518.125 kb on - strand at 2518.328 kb on + strand, within RR42_RS11780 at 2518.328 kb on + strand, within RR42_RS11780 at 2518.328 kb on + strand, within RR42_RS11780 at 2518.329 kb on - strand, within RR42_RS11780 at 2518.591 kb on - strand, within RR42_RS11780 at 2518.707 kb on + strand, within RR42_RS11780 at 2518.707 kb on + strand, within RR42_RS11780 at 2518.707 kb on + strand, within RR42_RS11780 at 2518.707 kb on + strand, within RR42_RS11780 at 2518.707 kb on + strand, within RR42_RS11780 at 2518.708 kb on - strand, within RR42_RS11780 at 2518.719 kb on - strand, within RR42_RS11780 at 2518.719 kb on - strand, within RR42_RS11780 at 2518.797 kb on + strand, within RR42_RS11780 at 2518.824 kb on - strand, within RR42_RS11780 at 2518.824 kb on - strand, within RR42_RS11780 at 2518.856 kb on + strand, within RR42_RS11780 at 2518.857 kb on - strand, within RR42_RS11780 at 2518.857 kb on - strand, within RR42_RS11780 at 2518.949 kb on + strand, within RR42_RS11780 at 2518.949 kb on + strand, within RR42_RS11780 at 2518.950 kb on - strand, within RR42_RS11780 at 2519.010 kb on - strand, within RR42_RS11780 at 2519.316 kb on + strand, within RR42_RS11785 at 2519.559 kb on + strand, within RR42_RS11785 at 2519.619 kb on + strand at 2519.620 kb on - strand at 2519.620 kb on - strand at 2519.620 kb on - strand at 2519.620 kb on - strand at 2519.648 kb on + strand at 2519.648 kb on + strand at 2519.649 kb on - strand at 2519.649 kb on - strand at 2519.649 kb on - strand at 2519.656 kb on + strand at 2519.656 kb on + strand at 2519.656 kb on + strand at 2519.664 kb on + strand at 2519.672 kb on + strand at 2519.762 kb on + strand at 2519.769 kb on - strand at 2519.811 kb on - strand at 2519.917 kb on + strand at 2519.917 kb on + strand at 2519.918 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 2,517,383 + RR42_RS11770 0.88 +0.8 2,517,383 + RR42_RS11770 0.88 -0.2 2,517,421 - +0.1 2,517,421 - +0.0 2,517,438 - +0.8 2,517,438 - +0.5 2,517,438 - +0.1 2,517,438 - +0.3 2,517,438 - +0.1 2,517,643 + -1.0 2,518,124 + +0.3 2,518,125 - -1.8 2,518,328 + RR42_RS11780 0.14 -1.5 2,518,328 + RR42_RS11780 0.14 -0.7 2,518,328 + RR42_RS11780 0.14 +0.1 2,518,329 - RR42_RS11780 0.14 -0.3 2,518,591 - RR42_RS11780 0.42 -0.1 2,518,707 + RR42_RS11780 0.54 +0.2 2,518,707 + RR42_RS11780 0.54 +0.8 2,518,707 + RR42_RS11780 0.54 -0.7 2,518,707 + RR42_RS11780 0.54 +0.4 2,518,707 + RR42_RS11780 0.54 -0.4 2,518,708 - RR42_RS11780 0.54 +0.6 2,518,719 - RR42_RS11780 0.56 -0.6 2,518,719 - RR42_RS11780 0.56 -2.5 2,518,797 + RR42_RS11780 0.64 -1.1 2,518,824 - RR42_RS11780 0.67 -0.9 2,518,824 - RR42_RS11780 0.67 +0.2 2,518,856 + RR42_RS11780 0.70 +0.2 2,518,857 - RR42_RS11780 0.70 +0.1 2,518,857 - RR42_RS11780 0.70 +0.2 2,518,949 + RR42_RS11780 0.80 -0.6 2,518,949 + RR42_RS11780 0.80 -0.5 2,518,950 - RR42_RS11780 0.80 -0.5 2,519,010 - RR42_RS11780 0.87 +0.5 2,519,316 + RR42_RS11785 0.26 -1.4 2,519,559 + RR42_RS11785 0.83 -0.3 2,519,619 + +0.9 2,519,620 - +0.3 2,519,620 - +1.2 2,519,620 - +0.0 2,519,620 - -0.5 2,519,648 + -1.0 2,519,648 + -0.9 2,519,649 - +0.8 2,519,649 - +0.4 2,519,649 - +0.7 2,519,656 + +0.4 2,519,656 + -0.3 2,519,656 + +0.1 2,519,664 + -0.9 2,519,672 + +1.3 2,519,762 + -0.6 2,519,769 - -0.4 2,519,811 - -1.4 2,519,917 + -2.8 2,519,917 + -1.4 2,519,918 - +0.8
Or see this region's nucleotide sequence