Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS09400 and RR42_RS09405 are separated by 176 nucleotides RR42_RS09405 and RR42_RS09410 are separated by 425 nucleotides
RR42_RS09400: RR42_RS09400 - membrane protein, at 1,991,924 to 1,993,072
_RS09400
RR42_RS09405: RR42_RS09405 - butanediol dehydrogenase, at 1,993,249 to 1,994,322
_RS09405
RR42_RS09410: RR42_RS09410 - hypothetical protein, at 1,994,748 to 1,996,640
_RS09410
Position (kb)
1993
1994
1995 Strain fitness (log2 ratio)
-2
-1
0
1 at 1992.309 kb on + strand, within RR42_RS09400 at 1992.309 kb on + strand, within RR42_RS09400 at 1992.359 kb on - strand, within RR42_RS09400 at 1992.425 kb on + strand, within RR42_RS09400 at 1992.426 kb on - strand, within RR42_RS09400 at 1992.426 kb on - strand, within RR42_RS09400 at 1992.438 kb on + strand, within RR42_RS09400 at 1992.510 kb on + strand, within RR42_RS09400 at 1992.525 kb on + strand, within RR42_RS09400 at 1992.526 kb on - strand, within RR42_RS09400 at 1992.526 kb on - strand, within RR42_RS09400 at 1992.645 kb on + strand, within RR42_RS09400 at 1992.645 kb on + strand, within RR42_RS09400 at 1992.879 kb on + strand, within RR42_RS09400 at 1993.051 kb on + strand at 1993.089 kb on - strand at 1993.089 kb on - strand at 1993.089 kb on - strand at 1993.430 kb on + strand, within RR42_RS09405 at 1993.430 kb on + strand, within RR42_RS09405 at 1993.431 kb on - strand, within RR42_RS09405 at 1993.466 kb on - strand, within RR42_RS09405 at 1993.605 kb on - strand, within RR42_RS09405 at 1993.901 kb on + strand, within RR42_RS09405 at 1993.902 kb on - strand, within RR42_RS09405 at 1994.009 kb on + strand, within RR42_RS09405 at 1994.189 kb on + strand, within RR42_RS09405 at 1994.189 kb on + strand, within RR42_RS09405 at 1994.189 kb on + strand, within RR42_RS09405 at 1994.190 kb on - strand, within RR42_RS09405 at 1994.240 kb on - strand at 1994.272 kb on + strand at 1994.272 kb on + strand at 1994.272 kb on + strand at 1994.273 kb on - strand at 1994.273 kb on - strand at 1994.372 kb on + strand at 1994.373 kb on - strand at 1994.572 kb on - strand at 1994.752 kb on + strand at 1994.765 kb on - strand at 1995.042 kb on + strand, within RR42_RS09410 at 1995.043 kb on - strand, within RR42_RS09410 at 1995.235 kb on + strand, within RR42_RS09410
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,992,309 + RR42_RS09400 0.34 +0.7 1,992,309 + RR42_RS09400 0.34 +0.2 1,992,359 - RR42_RS09400 0.38 +0.2 1,992,425 + RR42_RS09400 0.44 +0.0 1,992,426 - RR42_RS09400 0.44 +0.4 1,992,426 - RR42_RS09400 0.44 +0.2 1,992,438 + RR42_RS09400 0.45 -0.1 1,992,510 + RR42_RS09400 0.51 -0.6 1,992,525 + RR42_RS09400 0.52 -0.1 1,992,526 - RR42_RS09400 0.52 +0.2 1,992,526 - RR42_RS09400 0.52 +0.9 1,992,645 + RR42_RS09400 0.63 +0.0 1,992,645 + RR42_RS09400 0.63 +0.6 1,992,879 + RR42_RS09400 0.83 +0.2 1,993,051 + +0.4 1,993,089 - -1.2 1,993,089 - -0.4 1,993,089 - -1.8 1,993,430 + RR42_RS09405 0.17 +1.0 1,993,430 + RR42_RS09405 0.17 -0.3 1,993,431 - RR42_RS09405 0.17 -1.8 1,993,466 - RR42_RS09405 0.20 +1.4 1,993,605 - RR42_RS09405 0.33 +0.3 1,993,901 + RR42_RS09405 0.61 -1.1 1,993,902 - RR42_RS09405 0.61 +1.1 1,994,009 + RR42_RS09405 0.71 +0.0 1,994,189 + RR42_RS09405 0.88 +0.4 1,994,189 + RR42_RS09405 0.88 +0.4 1,994,189 + RR42_RS09405 0.88 -0.1 1,994,190 - RR42_RS09405 0.88 +0.3 1,994,240 - -0.1 1,994,272 + -1.3 1,994,272 + -1.0 1,994,272 + -1.3 1,994,273 - -1.0 1,994,273 - +0.3 1,994,372 + -0.1 1,994,373 - -0.3 1,994,572 - -0.4 1,994,752 + -1.6 1,994,765 - +0.0 1,995,042 + RR42_RS09410 0.16 -1.1 1,995,043 - RR42_RS09410 0.16 -0.6 1,995,235 + RR42_RS09410 0.26 +0.6
Or see this region's nucleotide sequence