Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS09065 and RR42_RS09070 are separated by 47 nucleotides RR42_RS09070 and RR42_RS09075 are separated by 2 nucleotides
RR42_RS09065: RR42_RS09065 - multidrug DMT transporter permease, at 1,906,488 to 1,907,399
_RS09065
RR42_RS09070: RR42_RS09070 - carbohydrate kinase, at 1,907,447 to 1,909,072
_RS09070
RR42_RS09075: RR42_RS09075 - amino acid dehydrogenase, at 1,909,075 to 1,910,373
_RS09075
Position (kb)
1907
1908
1909
1910 Strain fitness (log2 ratio)
-1
0
1 at 1906.621 kb on + strand, within RR42_RS09065 at 1906.687 kb on + strand, within RR42_RS09065 at 1906.712 kb on - strand, within RR42_RS09065 at 1907.266 kb on + strand, within RR42_RS09065 at 1907.266 kb on + strand, within RR42_RS09065 at 1907.267 kb on - strand, within RR42_RS09065 at 1907.267 kb on - strand, within RR42_RS09065 at 1907.500 kb on - strand at 1908.067 kb on + strand, within RR42_RS09070 at 1908.067 kb on + strand, within RR42_RS09070 at 1908.068 kb on - strand, within RR42_RS09070 at 1908.068 kb on - strand, within RR42_RS09070 at 1908.068 kb on - strand, within RR42_RS09070 at 1908.068 kb on - strand, within RR42_RS09070 at 1908.068 kb on - strand, within RR42_RS09070 at 1908.068 kb on - strand, within RR42_RS09070 at 1908.352 kb on - strand, within RR42_RS09070 at 1908.972 kb on + strand at 1909.087 kb on + strand at 1909.218 kb on - strand, within RR42_RS09075 at 1909.218 kb on - strand, within RR42_RS09075 at 1909.304 kb on + strand, within RR42_RS09075 at 1909.304 kb on + strand, within RR42_RS09075 at 1909.304 kb on + strand, within RR42_RS09075 at 1909.305 kb on - strand, within RR42_RS09075 at 1909.448 kb on + strand, within RR42_RS09075 at 1909.514 kb on + strand, within RR42_RS09075 at 1909.515 kb on - strand, within RR42_RS09075 at 1909.530 kb on - strand, within RR42_RS09075 at 1909.831 kb on - strand, within RR42_RS09075 at 1910.034 kb on - strand, within RR42_RS09075 at 1910.034 kb on - strand, within RR42_RS09075
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,906,621 + RR42_RS09065 0.15 +0.1 1,906,687 + RR42_RS09065 0.22 -0.2 1,906,712 - RR42_RS09065 0.25 -0.6 1,907,266 + RR42_RS09065 0.85 +0.3 1,907,266 + RR42_RS09065 0.85 -0.1 1,907,267 - RR42_RS09065 0.85 -0.3 1,907,267 - RR42_RS09065 0.85 +0.8 1,907,500 - -0.6 1,908,067 + RR42_RS09070 0.38 +0.3 1,908,067 + RR42_RS09070 0.38 +0.9 1,908,068 - RR42_RS09070 0.38 +0.1 1,908,068 - RR42_RS09070 0.38 -0.1 1,908,068 - RR42_RS09070 0.38 -0.1 1,908,068 - RR42_RS09070 0.38 -0.1 1,908,068 - RR42_RS09070 0.38 +1.3 1,908,068 - RR42_RS09070 0.38 -0.8 1,908,352 - RR42_RS09070 0.56 +0.2 1,908,972 + -1.0 1,909,087 + +0.4 1,909,218 - RR42_RS09075 0.11 +0.6 1,909,218 - RR42_RS09075 0.11 +0.4 1,909,304 + RR42_RS09075 0.18 +0.3 1,909,304 + RR42_RS09075 0.18 +0.6 1,909,304 + RR42_RS09075 0.18 +0.2 1,909,305 - RR42_RS09075 0.18 -0.2 1,909,448 + RR42_RS09075 0.29 -0.1 1,909,514 + RR42_RS09075 0.34 -0.3 1,909,515 - RR42_RS09075 0.34 -0.3 1,909,530 - RR42_RS09075 0.35 +0.2 1,909,831 - RR42_RS09075 0.58 -0.4 1,910,034 - RR42_RS09075 0.74 +0.1 1,910,034 - RR42_RS09075 0.74 +0.1
Or see this region's nucleotide sequence