Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS06520 and RR42_RS06525 are separated by 367 nucleotides RR42_RS06525 and RR42_RS06530 overlap by 4 nucleotides RR42_RS06530 and RR42_RS06535 are separated by 69 nucleotides
RR42_RS06520: RR42_RS06520 - short-chain dehydrogenase, at 1,427,288 to 1,428,073
_RS06520
RR42_RS06525: RR42_RS06525 - C4-dicarboxylate ABC transporter permease, at 1,428,441 to 1,429,049
_RS06525
RR42_RS06530: RR42_RS06530 - C4-dicarboxylate ABC transporter, at 1,429,046 to 1,430,350
_RS06530
RR42_RS06535: RR42_RS06535 - C4-dicarboxylate ABC transporter, at 1,430,420 to 1,431,448
_RS06535
Position (kb)
1429
1430
1431 Strain fitness (log2 ratio)
-2
-1
0
1 at 1428.071 kb on + strand at 1428.176 kb on + strand at 1428.344 kb on - strand at 1428.344 kb on - strand at 1428.544 kb on + strand, within RR42_RS06525 at 1428.545 kb on - strand, within RR42_RS06525 at 1428.628 kb on - strand, within RR42_RS06525 at 1428.628 kb on - strand, within RR42_RS06525 at 1428.864 kb on + strand, within RR42_RS06525 at 1428.864 kb on + strand, within RR42_RS06525 at 1428.865 kb on - strand, within RR42_RS06525 at 1428.865 kb on - strand, within RR42_RS06525 at 1428.865 kb on - strand, within RR42_RS06525 at 1428.865 kb on - strand, within RR42_RS06525 at 1428.913 kb on - strand, within RR42_RS06525 at 1428.967 kb on + strand, within RR42_RS06525 at 1429.223 kb on + strand, within RR42_RS06530 at 1429.223 kb on + strand, within RR42_RS06530 at 1429.224 kb on - strand, within RR42_RS06530 at 1429.224 kb on - strand, within RR42_RS06530 at 1429.224 kb on - strand, within RR42_RS06530 at 1429.224 kb on - strand, within RR42_RS06530 at 1429.538 kb on + strand, within RR42_RS06530 at 1429.538 kb on + strand, within RR42_RS06530 at 1429.538 kb on + strand, within RR42_RS06530 at 1429.539 kb on - strand, within RR42_RS06530 at 1429.539 kb on - strand, within RR42_RS06530 at 1429.539 kb on - strand, within RR42_RS06530 at 1429.539 kb on - strand, within RR42_RS06530 at 1429.540 kb on + strand, within RR42_RS06530 at 1429.540 kb on + strand, within RR42_RS06530 at 1429.540 kb on + strand, within RR42_RS06530 at 1429.657 kb on + strand, within RR42_RS06530 at 1429.658 kb on - strand, within RR42_RS06530 at 1429.658 kb on - strand, within RR42_RS06530 at 1429.658 kb on - strand, within RR42_RS06530 at 1429.808 kb on + strand, within RR42_RS06530 at 1429.809 kb on - strand, within RR42_RS06530 at 1429.836 kb on - strand, within RR42_RS06530 at 1430.336 kb on + strand at 1430.336 kb on + strand at 1430.337 kb on - strand at 1430.362 kb on + strand at 1430.383 kb on + strand at 1430.455 kb on + strand at 1430.455 kb on + strand at 1430.455 kb on + strand at 1430.456 kb on - strand at 1430.504 kb on + strand at 1430.505 kb on - strand at 1430.505 kb on - strand at 1430.505 kb on - strand at 1430.777 kb on + strand, within RR42_RS06535 at 1430.777 kb on + strand, within RR42_RS06535 at 1430.778 kb on - strand, within RR42_RS06535 at 1430.778 kb on - strand, within RR42_RS06535 at 1430.778 kb on - strand, within RR42_RS06535 at 1430.966 kb on + strand, within RR42_RS06535 at 1430.967 kb on - strand, within RR42_RS06535 at 1430.968 kb on + strand, within RR42_RS06535 at 1431.098 kb on + strand, within RR42_RS06535 at 1431.098 kb on + strand, within RR42_RS06535 at 1431.099 kb on - strand, within RR42_RS06535 at 1431.116 kb on + strand, within RR42_RS06535 at 1431.116 kb on + strand, within RR42_RS06535 at 1431.117 kb on - strand, within RR42_RS06535 at 1431.117 kb on - strand, within RR42_RS06535 at 1431.134 kb on + strand, within RR42_RS06535 at 1431.134 kb on + strand, within RR42_RS06535 at 1431.135 kb on - strand, within RR42_RS06535 at 1431.135 kb on - strand, within RR42_RS06535 at 1431.135 kb on - strand, within RR42_RS06535 at 1431.299 kb on - strand, within RR42_RS06535
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,428,071 + -0.9 1,428,176 + -0.4 1,428,344 - +1.1 1,428,344 - -0.6 1,428,544 + RR42_RS06525 0.17 +1.4 1,428,545 - RR42_RS06525 0.17 +0.1 1,428,628 - RR42_RS06525 0.31 +0.3 1,428,628 - RR42_RS06525 0.31 -0.4 1,428,864 + RR42_RS06525 0.69 -0.7 1,428,864 + RR42_RS06525 0.69 +0.1 1,428,865 - RR42_RS06525 0.70 +0.9 1,428,865 - RR42_RS06525 0.70 -0.7 1,428,865 - RR42_RS06525 0.70 +0.6 1,428,865 - RR42_RS06525 0.70 +0.2 1,428,913 - RR42_RS06525 0.78 +0.4 1,428,967 + RR42_RS06525 0.86 -0.5 1,429,223 + RR42_RS06530 0.14 -0.4 1,429,223 + RR42_RS06530 0.14 +0.7 1,429,224 - RR42_RS06530 0.14 +0.4 1,429,224 - RR42_RS06530 0.14 +0.1 1,429,224 - RR42_RS06530 0.14 -0.2 1,429,224 - RR42_RS06530 0.14 -0.8 1,429,538 + RR42_RS06530 0.38 -0.4 1,429,538 + RR42_RS06530 0.38 -0.9 1,429,538 + RR42_RS06530 0.38 -0.5 1,429,539 - RR42_RS06530 0.38 +1.0 1,429,539 - RR42_RS06530 0.38 +0.2 1,429,539 - RR42_RS06530 0.38 +0.4 1,429,539 - RR42_RS06530 0.38 -0.9 1,429,540 + RR42_RS06530 0.38 -0.1 1,429,540 + RR42_RS06530 0.38 -0.8 1,429,540 + RR42_RS06530 0.38 -0.1 1,429,657 + RR42_RS06530 0.47 +0.3 1,429,658 - RR42_RS06530 0.47 -0.3 1,429,658 - RR42_RS06530 0.47 -0.6 1,429,658 - RR42_RS06530 0.47 +1.2 1,429,808 + RR42_RS06530 0.58 -0.8 1,429,809 - RR42_RS06530 0.58 +0.4 1,429,836 - RR42_RS06530 0.61 -0.3 1,430,336 + -0.1 1,430,336 + +1.5 1,430,337 - -1.2 1,430,362 + -0.1 1,430,383 + +0.7 1,430,455 + +0.1 1,430,455 + -0.1 1,430,455 + +1.7 1,430,456 - +0.2 1,430,504 + -0.3 1,430,505 - -2.0 1,430,505 - +0.8 1,430,505 - +0.7 1,430,777 + RR42_RS06535 0.35 +1.7 1,430,777 + RR42_RS06535 0.35 +0.6 1,430,778 - RR42_RS06535 0.35 -1.2 1,430,778 - RR42_RS06535 0.35 -0.2 1,430,778 - RR42_RS06535 0.35 +0.1 1,430,966 + RR42_RS06535 0.53 +1.7 1,430,967 - RR42_RS06535 0.53 +0.7 1,430,968 + RR42_RS06535 0.53 -0.2 1,431,098 + RR42_RS06535 0.66 +0.5 1,431,098 + RR42_RS06535 0.66 -0.3 1,431,099 - RR42_RS06535 0.66 -0.8 1,431,116 + RR42_RS06535 0.68 +0.7 1,431,116 + RR42_RS06535 0.68 -1.7 1,431,117 - RR42_RS06535 0.68 +0.0 1,431,117 - RR42_RS06535 0.68 -0.3 1,431,134 + RR42_RS06535 0.69 +0.0 1,431,134 + RR42_RS06535 0.69 -0.5 1,431,135 - RR42_RS06535 0.69 +0.2 1,431,135 - RR42_RS06535 0.69 +1.1 1,431,135 - RR42_RS06535 0.69 -0.6 1,431,299 - RR42_RS06535 0.85 +0.5
Or see this region's nucleotide sequence