Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS06520 and RR42_RS06525 are separated by 367 nucleotides RR42_RS06525 and RR42_RS06530 overlap by 4 nucleotides
RR42_RS06520: RR42_RS06520 - short-chain dehydrogenase, at 1,427,288 to 1,428,073
_RS06520
RR42_RS06525: RR42_RS06525 - C4-dicarboxylate ABC transporter permease, at 1,428,441 to 1,429,049
_RS06525
RR42_RS06530: RR42_RS06530 - C4-dicarboxylate ABC transporter, at 1,429,046 to 1,430,350
_RS06530
Position (kb)
1428
1429
1430 Strain fitness (log2 ratio)
-1
0
1 at 1427.563 kb on + strand, within RR42_RS06520 at 1427.564 kb on - strand, within RR42_RS06520 at 1427.564 kb on - strand, within RR42_RS06520 at 1427.564 kb on - strand, within RR42_RS06520 at 1427.638 kb on + strand, within RR42_RS06520 at 1427.792 kb on + strand, within RR42_RS06520 at 1427.792 kb on + strand, within RR42_RS06520 at 1427.793 kb on - strand, within RR42_RS06520 at 1427.888 kb on + strand, within RR42_RS06520 at 1427.888 kb on + strand, within RR42_RS06520 at 1427.888 kb on + strand, within RR42_RS06520 at 1428.071 kb on + strand at 1428.176 kb on + strand at 1428.344 kb on - strand at 1428.344 kb on - strand at 1428.544 kb on + strand, within RR42_RS06525 at 1428.545 kb on - strand, within RR42_RS06525 at 1428.628 kb on - strand, within RR42_RS06525 at 1428.628 kb on - strand, within RR42_RS06525 at 1428.864 kb on + strand, within RR42_RS06525 at 1428.864 kb on + strand, within RR42_RS06525 at 1428.865 kb on - strand, within RR42_RS06525 at 1428.865 kb on - strand, within RR42_RS06525 at 1428.865 kb on - strand, within RR42_RS06525 at 1428.865 kb on - strand, within RR42_RS06525 at 1428.913 kb on - strand, within RR42_RS06525 at 1428.967 kb on + strand, within RR42_RS06525 at 1429.223 kb on + strand, within RR42_RS06530 at 1429.223 kb on + strand, within RR42_RS06530 at 1429.224 kb on - strand, within RR42_RS06530 at 1429.224 kb on - strand, within RR42_RS06530 at 1429.224 kb on - strand, within RR42_RS06530 at 1429.224 kb on - strand, within RR42_RS06530 at 1429.538 kb on + strand, within RR42_RS06530 at 1429.538 kb on + strand, within RR42_RS06530 at 1429.538 kb on + strand, within RR42_RS06530 at 1429.539 kb on - strand, within RR42_RS06530 at 1429.539 kb on - strand, within RR42_RS06530 at 1429.539 kb on - strand, within RR42_RS06530 at 1429.539 kb on - strand, within RR42_RS06530 at 1429.540 kb on + strand, within RR42_RS06530 at 1429.540 kb on + strand, within RR42_RS06530 at 1429.540 kb on + strand, within RR42_RS06530 at 1429.657 kb on + strand, within RR42_RS06530 at 1429.658 kb on - strand, within RR42_RS06530 at 1429.658 kb on - strand, within RR42_RS06530 at 1429.658 kb on - strand, within RR42_RS06530 at 1429.808 kb on + strand, within RR42_RS06530 at 1429.809 kb on - strand, within RR42_RS06530 at 1429.836 kb on - strand, within RR42_RS06530
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,427,563 + RR42_RS06520 0.35 +0.5 1,427,564 - RR42_RS06520 0.35 +0.0 1,427,564 - RR42_RS06520 0.35 +0.2 1,427,564 - RR42_RS06520 0.35 +0.2 1,427,638 + RR42_RS06520 0.45 +0.3 1,427,792 + RR42_RS06520 0.64 +0.4 1,427,792 + RR42_RS06520 0.64 +0.1 1,427,793 - RR42_RS06520 0.64 +0.5 1,427,888 + RR42_RS06520 0.76 +0.9 1,427,888 + RR42_RS06520 0.76 +0.7 1,427,888 + RR42_RS06520 0.76 +0.3 1,428,071 + -0.9 1,428,176 + -0.4 1,428,344 - +1.1 1,428,344 - -0.6 1,428,544 + RR42_RS06525 0.17 +1.4 1,428,545 - RR42_RS06525 0.17 +0.1 1,428,628 - RR42_RS06525 0.31 +0.3 1,428,628 - RR42_RS06525 0.31 -0.4 1,428,864 + RR42_RS06525 0.69 -0.7 1,428,864 + RR42_RS06525 0.69 +0.1 1,428,865 - RR42_RS06525 0.70 +0.9 1,428,865 - RR42_RS06525 0.70 -0.7 1,428,865 - RR42_RS06525 0.70 +0.6 1,428,865 - RR42_RS06525 0.70 +0.2 1,428,913 - RR42_RS06525 0.78 +0.4 1,428,967 + RR42_RS06525 0.86 -0.5 1,429,223 + RR42_RS06530 0.14 -0.4 1,429,223 + RR42_RS06530 0.14 +0.7 1,429,224 - RR42_RS06530 0.14 +0.4 1,429,224 - RR42_RS06530 0.14 +0.1 1,429,224 - RR42_RS06530 0.14 -0.2 1,429,224 - RR42_RS06530 0.14 -0.8 1,429,538 + RR42_RS06530 0.38 -0.4 1,429,538 + RR42_RS06530 0.38 -0.9 1,429,538 + RR42_RS06530 0.38 -0.5 1,429,539 - RR42_RS06530 0.38 +1.0 1,429,539 - RR42_RS06530 0.38 +0.2 1,429,539 - RR42_RS06530 0.38 +0.4 1,429,539 - RR42_RS06530 0.38 -0.9 1,429,540 + RR42_RS06530 0.38 -0.1 1,429,540 + RR42_RS06530 0.38 -0.8 1,429,540 + RR42_RS06530 0.38 -0.1 1,429,657 + RR42_RS06530 0.47 +0.3 1,429,658 - RR42_RS06530 0.47 -0.3 1,429,658 - RR42_RS06530 0.47 -0.6 1,429,658 - RR42_RS06530 0.47 +1.2 1,429,808 + RR42_RS06530 0.58 -0.8 1,429,809 - RR42_RS06530 0.58 +0.4 1,429,836 - RR42_RS06530 0.61 -0.3
Or see this region's nucleotide sequence