Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03940 and RR42_RS03945 are separated by 181 nucleotides RR42_RS03945 and RR42_RS03950 are separated by 17 nucleotides RR42_RS03950 and RR42_RS03955 are separated by 208 nucleotides
RR42_RS03940: RR42_RS03940 - membrane protein, at 868,872 to 870,041
_RS03940
RR42_RS03945: RR42_RS03945 - chloride channel protein, at 870,223 to 871,962
_RS03945
RR42_RS03950: RR42_RS03950 - AsnC family transcriptional regulator, at 871,980 to 872,402
_RS03950
RR42_RS03955: RR42_RS03955 - amidinotransferase, at 872,611 to 873,510
_RS03955
Position (kb) 870
871
872 Strain fitness (log2 ratio) -1
0
1 at 869.314 kb on + strand, within RR42_RS03940 at 869.315 kb on - strand, within RR42_RS03940 at 870.149 kb on - strand at 870.365 kb on - strand at 870.365 kb on - strand at 871.500 kb on + strand, within RR42_RS03945 at 871.501 kb on - strand, within RR42_RS03945 at 871.501 kb on - strand, within RR42_RS03945 at 871.501 kb on - strand, within RR42_RS03945 at 871.501 kb on - strand, within RR42_RS03945 at 871.501 kb on - strand, within RR42_RS03945 at 871.504 kb on + strand, within RR42_RS03945 at 871.596 kb on - strand, within RR42_RS03945 at 871.982 kb on - strand at 871.991 kb on - strand at 871.991 kb on - strand at 872.653 kb on + strand at 872.653 kb on + strand at 872.654 kb on - strand at 872.654 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 869,314 + RR42_RS03940 0.38 +1.7 869,315 - RR42_RS03940 0.38 +0.2 870,149 - +0.9 870,365 - -1.0 870,365 - +0.4 871,500 + RR42_RS03945 0.73 -0.2 871,501 - RR42_RS03945 0.73 -0.3 871,501 - RR42_RS03945 0.73 +0.1 871,501 - RR42_RS03945 0.73 -0.5 871,501 - RR42_RS03945 0.73 -1.3 871,501 - RR42_RS03945 0.73 -1.7 871,504 + RR42_RS03945 0.74 +0.7 871,596 - RR42_RS03945 0.79 -0.5 871,982 - +0.7 871,991 - +1.1 871,991 - +0.4 872,653 + -0.3 872,653 + -0.8 872,654 - -0.5 872,654 - -0.1
Or see this region's nucleotide sequence