Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02525 and RR42_RS02530 are separated by 26 nucleotides RR42_RS02530 and RR42_RS02535 are separated by 177 nucleotides RR42_RS02535 and RR42_RS02540 are separated by 296 nucleotides
RR42_RS02525: RR42_RS02525 - enoyl-CoA hydratase, at 568,689 to 569,465
_RS02525
RR42_RS02530: RR42_RS02530 - acyl-CoA hydrolase, at 569,492 to 569,914
_RS02530
RR42_RS02535: RR42_RS02535 - metal ABC transporter permease, at 570,092 to 571,987
_RS02535
RR42_RS02540: RR42_RS02540 - taurine ABC transporter permease, at 572,284 to 573,306
_RS02540
Position (kb) 570
571
572 Strain fitness (log2 ratio) -2
-1
0
1
2 at 569.276 kb on - strand, within RR42_RS02525 at 569.464 kb on - strand at 569.464 kb on - strand at 569.464 kb on - strand at 569.542 kb on + strand, within RR42_RS02530 at 569.542 kb on + strand, within RR42_RS02530 at 569.559 kb on + strand, within RR42_RS02530 at 569.559 kb on + strand, within RR42_RS02530 at 569.559 kb on + strand, within RR42_RS02530 at 569.559 kb on + strand, within RR42_RS02530 at 569.560 kb on - strand, within RR42_RS02530 at 569.619 kb on + strand, within RR42_RS02530 at 569.620 kb on - strand, within RR42_RS02530 at 569.633 kb on + strand, within RR42_RS02530 at 570.042 kb on + strand at 570.046 kb on + strand at 570.046 kb on + strand at 570.071 kb on + strand at 570.091 kb on + strand at 570.101 kb on + strand at 570.102 kb on - strand at 570.106 kb on + strand at 570.106 kb on + strand at 570.107 kb on - strand at 570.107 kb on - strand at 570.203 kb on + strand at 570.203 kb on + strand at 570.203 kb on + strand at 570.216 kb on - strand at 570.216 kb on - strand at 570.593 kb on + strand, within RR42_RS02535 at 570.653 kb on + strand, within RR42_RS02535 at 570.653 kb on + strand, within RR42_RS02535 at 570.653 kb on + strand, within RR42_RS02535 at 570.659 kb on - strand, within RR42_RS02535 at 570.698 kb on + strand, within RR42_RS02535 at 570.698 kb on + strand, within RR42_RS02535 at 570.698 kb on + strand, within RR42_RS02535 at 570.699 kb on - strand, within RR42_RS02535 at 570.699 kb on - strand, within RR42_RS02535 at 570.699 kb on - strand, within RR42_RS02535 at 570.699 kb on - strand, within RR42_RS02535 at 570.699 kb on - strand, within RR42_RS02535 at 570.699 kb on - strand, within RR42_RS02535 at 570.700 kb on + strand, within RR42_RS02535 at 570.701 kb on - strand, within RR42_RS02535 at 570.840 kb on - strand, within RR42_RS02535 at 570.840 kb on - strand, within RR42_RS02535 at 571.050 kb on - strand, within RR42_RS02535 at 571.346 kb on + strand, within RR42_RS02535 at 571.346 kb on + strand, within RR42_RS02535 at 571.346 kb on + strand, within RR42_RS02535 at 571.469 kb on + strand, within RR42_RS02535 at 571.469 kb on + strand, within RR42_RS02535 at 571.469 kb on + strand, within RR42_RS02535 at 571.473 kb on - strand, within RR42_RS02535 at 571.473 kb on - strand, within RR42_RS02535 at 571.574 kb on + strand, within RR42_RS02535 at 572.079 kb on + strand at 572.079 kb on + strand at 572.080 kb on - strand at 572.080 kb on - strand at 572.089 kb on - strand at 572.157 kb on - strand at 572.157 kb on - strand at 572.157 kb on - strand at 572.181 kb on - strand at 572.434 kb on + strand, within RR42_RS02540 at 572.435 kb on - strand, within RR42_RS02540 at 572.609 kb on - strand, within RR42_RS02540 at 572.917 kb on + strand, within RR42_RS02540
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 569,276 - RR42_RS02525 0.76 -0.9 569,464 - -0.3 569,464 - -2.7 569,464 - -0.3 569,542 + RR42_RS02530 0.12 +0.4 569,542 + RR42_RS02530 0.12 -1.5 569,559 + RR42_RS02530 0.16 -0.1 569,559 + RR42_RS02530 0.16 +0.3 569,559 + RR42_RS02530 0.16 -0.3 569,559 + RR42_RS02530 0.16 +0.2 569,560 - RR42_RS02530 0.16 -0.1 569,619 + RR42_RS02530 0.30 +0.9 569,620 - RR42_RS02530 0.30 -1.2 569,633 + RR42_RS02530 0.33 +0.1 570,042 + +1.0 570,046 + +0.0 570,046 + +1.0 570,071 + -0.7 570,091 + +0.5 570,101 + -0.4 570,102 - +0.5 570,106 + +0.3 570,106 + -0.0 570,107 - +0.0 570,107 - -0.1 570,203 + +0.6 570,203 + +2.1 570,203 + -0.7 570,216 - -0.2 570,216 - -0.1 570,593 + RR42_RS02535 0.26 +0.8 570,653 + RR42_RS02535 0.30 +0.4 570,653 + RR42_RS02535 0.30 -0.4 570,653 + RR42_RS02535 0.30 +0.8 570,659 - RR42_RS02535 0.30 +0.8 570,698 + RR42_RS02535 0.32 -0.7 570,698 + RR42_RS02535 0.32 +0.1 570,698 + RR42_RS02535 0.32 +0.5 570,699 - RR42_RS02535 0.32 +0.1 570,699 - RR42_RS02535 0.32 +1.1 570,699 - RR42_RS02535 0.32 +0.5 570,699 - RR42_RS02535 0.32 -0.4 570,699 - RR42_RS02535 0.32 +0.2 570,699 - RR42_RS02535 0.32 -0.0 570,700 + RR42_RS02535 0.32 +0.7 570,701 - RR42_RS02535 0.32 -1.2 570,840 - RR42_RS02535 0.39 -0.1 570,840 - RR42_RS02535 0.39 -0.1 571,050 - RR42_RS02535 0.51 -1.3 571,346 + RR42_RS02535 0.66 +0.9 571,346 + RR42_RS02535 0.66 +0.2 571,346 + RR42_RS02535 0.66 +0.7 571,469 + RR42_RS02535 0.73 -0.5 571,469 + RR42_RS02535 0.73 +0.6 571,469 + RR42_RS02535 0.73 +0.8 571,473 - RR42_RS02535 0.73 -0.0 571,473 - RR42_RS02535 0.73 +0.5 571,574 + RR42_RS02535 0.78 +0.0 572,079 + +0.6 572,079 + -0.4 572,080 - +0.3 572,080 - +1.8 572,089 - -0.7 572,157 - +0.3 572,157 - +0.3 572,157 - -0.1 572,181 - +0.0 572,434 + RR42_RS02540 0.15 +0.0 572,435 - RR42_RS02540 0.15 +0.3 572,609 - RR42_RS02540 0.32 +0.3 572,917 + RR42_RS02540 0.62 +1.7
Or see this region's nucleotide sequence