Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02510 and RR42_RS02515 are separated by 18 nucleotides RR42_RS02515 and RR42_RS02520 are separated by 144 nucleotides
RR42_RS02510: RR42_RS02510 - 3-hydroxyacyl-CoA dehydrogenase, at 563,889 to 566,315
_RS02510
RR42_RS02515: RR42_RS02515 - acetyl-CoA acetyltransferase, at 566,334 to 567,533
_RS02515
RR42_RS02520: RR42_RS02520 - hypothetical protein, at 567,678 to 568,634
_RS02520
Position (kb) 566
567
568 Strain fitness (log2 ratio) -1
0
1 at 565.340 kb on - strand, within RR42_RS02510 at 565.340 kb on - strand, within RR42_RS02510 at 565.635 kb on + strand, within RR42_RS02510 at 565.635 kb on + strand, within RR42_RS02510 at 565.737 kb on + strand, within RR42_RS02510 at 565.737 kb on + strand, within RR42_RS02510 at 565.737 kb on + strand, within RR42_RS02510 at 565.737 kb on + strand, within RR42_RS02510 at 565.738 kb on - strand, within RR42_RS02510 at 565.846 kb on - strand, within RR42_RS02510 at 565.846 kb on - strand, within RR42_RS02510 at 566.358 kb on + strand at 566.358 kb on + strand at 566.358 kb on + strand at 566.358 kb on + strand at 566.358 kb on + strand at 566.359 kb on - strand at 566.359 kb on - strand at 567.469 kb on - strand at 567.515 kb on + strand at 567.575 kb on + strand at 567.596 kb on + strand at 567.621 kb on - strand at 567.621 kb on - strand at 567.828 kb on + strand, within RR42_RS02520 at 567.828 kb on + strand, within RR42_RS02520 at 567.978 kb on + strand, within RR42_RS02520 at 567.979 kb on - strand, within RR42_RS02520 at 567.979 kb on - strand, within RR42_RS02520 at 568.202 kb on + strand, within RR42_RS02520 at 568.202 kb on + strand, within RR42_RS02520 at 568.203 kb on - strand, within RR42_RS02520 at 568.230 kb on + strand, within RR42_RS02520 at 568.231 kb on - strand, within RR42_RS02520 at 568.236 kb on + strand, within RR42_RS02520 at 568.236 kb on + strand, within RR42_RS02520 at 568.237 kb on - strand, within RR42_RS02520 at 568.284 kb on + strand, within RR42_RS02520 at 568.326 kb on - strand, within RR42_RS02520 at 568.326 kb on - strand, within RR42_RS02520 at 568.455 kb on + strand, within RR42_RS02520 at 568.455 kb on + strand, within RR42_RS02520 at 568.494 kb on + strand, within RR42_RS02520 at 568.495 kb on - strand, within RR42_RS02520
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 565,340 - RR42_RS02510 0.60 +0.6 565,340 - RR42_RS02510 0.60 +0.2 565,635 + RR42_RS02510 0.72 +0.3 565,635 + RR42_RS02510 0.72 +1.1 565,737 + RR42_RS02510 0.76 +0.9 565,737 + RR42_RS02510 0.76 +0.1 565,737 + RR42_RS02510 0.76 -1.5 565,737 + RR42_RS02510 0.76 +0.7 565,738 - RR42_RS02510 0.76 +0.7 565,846 - RR42_RS02510 0.81 +0.4 565,846 - RR42_RS02510 0.81 -0.3 566,358 + -0.0 566,358 + +0.8 566,358 + +0.7 566,358 + -0.9 566,358 + +0.9 566,359 - -0.6 566,359 - -0.6 567,469 - +0.2 567,515 + +0.5 567,575 + +0.8 567,596 + +0.7 567,621 - +0.7 567,621 - +0.1 567,828 + RR42_RS02520 0.16 -0.2 567,828 + RR42_RS02520 0.16 -0.6 567,978 + RR42_RS02520 0.31 -0.4 567,979 - RR42_RS02520 0.31 -0.1 567,979 - RR42_RS02520 0.31 -0.7 568,202 + RR42_RS02520 0.55 +0.1 568,202 + RR42_RS02520 0.55 -1.4 568,203 - RR42_RS02520 0.55 +0.2 568,230 + RR42_RS02520 0.58 -0.3 568,231 - RR42_RS02520 0.58 +0.9 568,236 + RR42_RS02520 0.58 +0.9 568,236 + RR42_RS02520 0.58 -0.9 568,237 - RR42_RS02520 0.58 +0.2 568,284 + RR42_RS02520 0.63 -0.6 568,326 - RR42_RS02520 0.68 +1.4 568,326 - RR42_RS02520 0.68 -0.4 568,455 + RR42_RS02520 0.81 +0.2 568,455 + RR42_RS02520 0.81 +1.5 568,494 + RR42_RS02520 0.85 +0.9 568,495 - RR42_RS02520 0.85 +0.5
Or see this region's nucleotide sequence