Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02280 and RR42_RS02285 are separated by 61 nucleotides RR42_RS02285 and RR42_RS02290 overlap by 4 nucleotides
RR42_RS02280: RR42_RS02280 - ABC transporter permease, at 510,359 to 511,807
_RS02280
RR42_RS02285: RR42_RS02285 - mannose-1-phosphate guanyltransferase, at 511,869 to 513,047
_RS02285
RR42_RS02290: RR42_RS02290 - multidrug ABC transporter ATP-binding protein, at 513,044 to 514,057
_RS02290
Position (kb)
511
512
513
514 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 511.035 kb on + strand, within RR42_RS02280 at 511.035 kb on + strand, within RR42_RS02280 at 511.035 kb on + strand, within RR42_RS02280 at 511.036 kb on - strand, within RR42_RS02280 at 511.374 kb on + strand, within RR42_RS02280 at 511.838 kb on - strand at 511.953 kb on + strand at 512.009 kb on - strand, within RR42_RS02285 at 512.082 kb on + strand, within RR42_RS02285 at 512.105 kb on - strand, within RR42_RS02285 at 512.105 kb on - strand, within RR42_RS02285 at 512.105 kb on - strand, within RR42_RS02285 at 512.206 kb on + strand, within RR42_RS02285 at 512.206 kb on + strand, within RR42_RS02285 at 512.206 kb on + strand, within RR42_RS02285 at 512.299 kb on + strand, within RR42_RS02285 at 512.299 kb on + strand, within RR42_RS02285 at 512.299 kb on + strand, within RR42_RS02285 at 512.299 kb on + strand, within RR42_RS02285 at 512.299 kb on + strand, within RR42_RS02285 at 512.300 kb on - strand, within RR42_RS02285 at 512.458 kb on + strand, within RR42_RS02285 at 512.459 kb on - strand, within RR42_RS02285 at 512.459 kb on - strand, within RR42_RS02285 at 512.773 kb on + strand, within RR42_RS02285 at 512.774 kb on - strand, within RR42_RS02285 at 512.774 kb on - strand, within RR42_RS02285 at 512.774 kb on - strand, within RR42_RS02285 at 513.232 kb on + strand, within RR42_RS02290 at 513.394 kb on - strand, within RR42_RS02290 at 513.401 kb on - strand, within RR42_RS02290 at 513.402 kb on + strand, within RR42_RS02290 at 513.403 kb on - strand, within RR42_RS02290 at 513.439 kb on - strand, within RR42_RS02290 at 513.439 kb on - strand, within RR42_RS02290 at 513.699 kb on + strand, within RR42_RS02290 at 513.700 kb on - strand, within RR42_RS02290 at 513.771 kb on + strand, within RR42_RS02290 at 513.771 kb on + strand, within RR42_RS02290 at 513.772 kb on - strand, within RR42_RS02290 at 513.973 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 511,035 + RR42_RS02280 0.47 +0.4 511,035 + RR42_RS02280 0.47 -0.8 511,035 + RR42_RS02280 0.47 -0.4 511,036 - RR42_RS02280 0.47 +0.2 511,374 + RR42_RS02280 0.70 -0.4 511,838 - -0.1 511,953 + -0.1 512,009 - RR42_RS02285 0.12 +0.5 512,082 + RR42_RS02285 0.18 -0.9 512,105 - RR42_RS02285 0.20 +0.4 512,105 - RR42_RS02285 0.20 -0.2 512,105 - RR42_RS02285 0.20 -0.2 512,206 + RR42_RS02285 0.29 -0.0 512,206 + RR42_RS02285 0.29 +1.4 512,206 + RR42_RS02285 0.29 +0.3 512,299 + RR42_RS02285 0.36 +1.0 512,299 + RR42_RS02285 0.36 -0.4 512,299 + RR42_RS02285 0.36 +0.4 512,299 + RR42_RS02285 0.36 +0.1 512,299 + RR42_RS02285 0.36 -1.6 512,300 - RR42_RS02285 0.37 +1.5 512,458 + RR42_RS02285 0.50 +0.5 512,459 - RR42_RS02285 0.50 +0.1 512,459 - RR42_RS02285 0.50 +4.0 512,773 + RR42_RS02285 0.77 +0.0 512,774 - RR42_RS02285 0.77 +1.1 512,774 - RR42_RS02285 0.77 -0.0 512,774 - RR42_RS02285 0.77 +2.0 513,232 + RR42_RS02290 0.19 +0.0 513,394 - RR42_RS02290 0.35 +1.1 513,401 - RR42_RS02290 0.35 +0.1 513,402 + RR42_RS02290 0.35 +0.9 513,403 - RR42_RS02290 0.35 +0.4 513,439 - RR42_RS02290 0.39 +0.5 513,439 - RR42_RS02290 0.39 +1.8 513,699 + RR42_RS02290 0.65 -1.2 513,700 - RR42_RS02290 0.65 +0.2 513,771 + RR42_RS02290 0.72 +0.3 513,771 + RR42_RS02290 0.72 -0.1 513,772 - RR42_RS02290 0.72 +0.4 513,973 - +0.3
Or see this region's nucleotide sequence