Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00875 and RR42_RS00880 overlap by 4 nucleotides RR42_RS00880 and RR42_RS00885 are separated by 93 nucleotides RR42_RS00885 and RR42_RS00890 are separated by 84 nucleotides RR42_RS00890 and RR42_RS00895 are separated by 48 nucleotides
RR42_RS00875: RR42_RS00875 - isocitrate dehydrogenase, at 211,201 to 213,045
_RS00875
RR42_RS00880: RR42_RS00880 - carbonate dehydratase, at 213,042 to 213,713
_RS00880
RR42_RS00885: RR42_RS00885 - acetyl-CoA acetyltransferase, at 213,807 to 215,003
_RS00885
RR42_RS00890: RR42_RS00890 - short-chain dehydrogenase, at 215,088 to 215,768
_RS00890
RR42_RS00895: RR42_RS00895 - acyl-CoA dehydrogenase, at 215,817 to 216,947
_RS00895
Position (kb) 213
214
215
216 Strain fitness (log2 ratio) -2
-1
0
1 at 212.941 kb on + strand at 212.941 kb on + strand at 212.941 kb on + strand at 212.941 kb on + strand at 213.739 kb on + strand at 213.739 kb on + strand at 213.743 kb on + strand at 213.743 kb on + strand at 213.744 kb on - strand at 213.820 kb on + strand at 213.820 kb on + strand at 214.263 kb on + strand, within RR42_RS00885 at 214.264 kb on - strand, within RR42_RS00885 at 214.264 kb on - strand, within RR42_RS00885 at 214.329 kb on + strand, within RR42_RS00885 at 214.329 kb on + strand, within RR42_RS00885 at 214.329 kb on + strand, within RR42_RS00885 at 214.329 kb on + strand, within RR42_RS00885 at 214.329 kb on + strand, within RR42_RS00885 at 214.329 kb on + strand, within RR42_RS00885 at 214.338 kb on - strand, within RR42_RS00885 at 215.067 kb on + strand at 215.305 kb on + strand, within RR42_RS00890 at 215.306 kb on - strand, within RR42_RS00890 at 215.306 kb on - strand, within RR42_RS00890 at 215.313 kb on + strand, within RR42_RS00890 at 215.313 kb on + strand, within RR42_RS00890 at 215.313 kb on + strand, within RR42_RS00890 at 215.742 kb on + strand at 215.742 kb on + strand at 215.742 kb on + strand at 215.742 kb on + strand at 215.742 kb on + strand at 215.742 kb on + strand at 215.743 kb on - strand at 215.743 kb on - strand at 215.743 kb on - strand at 215.743 kb on - strand at 215.743 kb on - strand at 215.961 kb on + strand, within RR42_RS00895 at 215.985 kb on + strand, within RR42_RS00895
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 212,941 + -1.1 212,941 + -0.4 212,941 + +0.4 212,941 + -0.8 213,739 + -1.0 213,739 + -1.8 213,743 + +0.6 213,743 + +0.4 213,744 - -2.2 213,820 + +0.8 213,820 + +0.1 214,263 + RR42_RS00885 0.38 -1.7 214,264 - RR42_RS00885 0.38 -0.4 214,264 - RR42_RS00885 0.38 -1.1 214,329 + RR42_RS00885 0.44 -0.9 214,329 + RR42_RS00885 0.44 -0.3 214,329 + RR42_RS00885 0.44 +0.5 214,329 + RR42_RS00885 0.44 -1.0 214,329 + RR42_RS00885 0.44 -0.5 214,329 + RR42_RS00885 0.44 -1.0 214,338 - RR42_RS00885 0.44 -2.2 215,067 + -0.1 215,305 + RR42_RS00890 0.32 -2.7 215,306 - RR42_RS00890 0.32 +1.1 215,306 - RR42_RS00890 0.32 -1.0 215,313 + RR42_RS00890 0.33 +0.1 215,313 + RR42_RS00890 0.33 -0.1 215,313 + RR42_RS00890 0.33 -0.9 215,742 + +0.6 215,742 + -0.6 215,742 + -0.8 215,742 + -0.8 215,742 + +0.2 215,742 + +1.0 215,743 - -0.1 215,743 - +0.1 215,743 - -0.1 215,743 - -0.1 215,743 - -0.1 215,961 + RR42_RS00895 0.13 +0.2 215,985 + RR42_RS00895 0.15 +1.2
Or see this region's nucleotide sequence