Experiment: casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS25510 and EX31_RS26060 overlap by 4 nucleotides EX31_RS26060 and EX31_RS17040 overlap by 58 nucleotides EX31_RS17040 and EX31_RS26065 are separated by 85 nucleotides EX31_RS26065 and EX31_RS17045 are separated by 108 nucleotides EX31_RS17045 and EX31_RS17050 overlap by 1 nucleotides EX31_RS17050 and EX31_RS17055 are separated by 10 nucleotides
EX31_RS25510: EX31_RS25510 - IS3 family transposase, at 55 to 453
_RS25510
EX31_RS26060: EX31_RS26060 - hypothetical protein, at 450 to 575
_RS26060
EX31_RS17040: EX31_RS17040 - transposase, at 518 to 715
_RS17040
EX31_RS26065: EX31_RS26065 - IS630 family transposase, at 801 to 908
_RS26065
EX31_RS17045: EX31_RS17045 - c-type cytochrome, at 1,017 to 1,331
_RS17045
EX31_RS17050: EX31_RS17050 - TlpA family protein disulfide reductase, at 1,331 to 1,804
_RS17050
EX31_RS17055: EX31_RS17055 - ABC transporter ATP-binding protein, at 1,815 to 2,534
_RS17055
Position (kb) 0
1 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 0.849 kb on - strand, within EX31_RS26065 at 0.849 kb on - strand, within EX31_RS26065 at 0.897 kb on + strand, within EX31_RS26065 at 0.897 kb on + strand, within EX31_RS26065 at 0.897 kb on + strand, within EX31_RS26065 at 0.898 kb on - strand at 0.898 kb on - strand at 0.898 kb on - strand at 0.898 kb on - strand at 0.908 kb on + strand at 0.909 kb on - strand at 0.909 kb on - strand at 0.909 kb on - strand at 0.909 kb on - strand at 0.959 kb on + strand at 0.959 kb on + strand at 0.984 kb on + strand at 0.984 kb on + strand at 0.984 kb on + strand at 0.984 kb on + strand at 0.985 kb on - strand at 0.985 kb on - strand at 0.985 kb on - strand at 0.989 kb on + strand at 0.989 kb on + strand at 0.992 kb on + strand at 0.992 kb on + strand at 0.993 kb on - strand at 0.993 kb on - strand at 0.993 kb on - strand at 1.005 kb on + strand at 1.006 kb on - strand at 1.006 kb on - strand at 1.006 kb on - strand at 1.009 kb on + strand at 1.010 kb on - strand at 1.018 kb on + strand at 1.018 kb on + strand at 1.018 kb on + strand at 1.018 kb on + strand at 1.019 kb on - strand at 1.019 kb on - strand at 1.019 kb on - strand at 1.027 kb on - strand at 1.047 kb on + strand at 1.070 kb on + strand, within EX31_RS17045 at 1.097 kb on + strand, within EX31_RS17045 at 1.098 kb on - strand, within EX31_RS17045 at 1.104 kb on + strand, within EX31_RS17045 at 1.104 kb on + strand, within EX31_RS17045 at 1.104 kb on + strand, within EX31_RS17045 at 1.104 kb on + strand, within EX31_RS17045 at 1.105 kb on - strand, within EX31_RS17045 at 1.105 kb on - strand, within EX31_RS17045 at 1.105 kb on - strand, within EX31_RS17045 at 1.105 kb on - strand, within EX31_RS17045 at 1.234 kb on + strand, within EX31_RS17045 at 1.234 kb on + strand, within EX31_RS17045 at 1.234 kb on + strand, within EX31_RS17045 at 1.234 kb on + strand, within EX31_RS17045 at 1.234 kb on + strand, within EX31_RS17045 at 1.234 kb on + strand, within EX31_RS17045 at 1.235 kb on - strand, within EX31_RS17045 at 1.235 kb on - strand, within EX31_RS17045 at 1.235 kb on - strand, within EX31_RS17045 at 1.235 kb on - strand, within EX31_RS17045 at 1.235 kb on - strand, within EX31_RS17045 at 1.235 kb on - strand, within EX31_RS17045 at 1.235 kb on - strand, within EX31_RS17045 at 1.235 kb on - strand, within EX31_RS17045 at 1.235 kb on - strand, within EX31_RS17045 at 1.235 kb on - strand, within EX31_RS17045 at 1.235 kb on - strand, within EX31_RS17045 at 1.266 kb on - strand, within EX31_RS17045 at 1.317 kb on + strand at 1.318 kb on - strand at 1.333 kb on - strand at 1.333 kb on - strand at 1.333 kb on - strand at 1.333 kb on - strand at 1.358 kb on + strand at 1.358 kb on + strand at 1.359 kb on - strand at 1.359 kb on - strand at 1.359 kb on - strand at 1.359 kb on - strand at 1.359 kb on - strand at 1.367 kb on + strand at 1.445 kb on - strand, within EX31_RS17050 at 1.445 kb on - strand, within EX31_RS17050 at 1.445 kb on - strand, within EX31_RS17050 at 1.445 kb on - strand, within EX31_RS17050 at 1.458 kb on + strand, within EX31_RS17050 at 1.459 kb on - strand, within EX31_RS17050 at 1.555 kb on + strand, within EX31_RS17050 at 1.556 kb on - strand, within EX31_RS17050 at 1.556 kb on - strand, within EX31_RS17050 at 1.556 kb on - strand, within EX31_RS17050 at 1.556 kb on - strand, within EX31_RS17050 at 1.556 kb on - strand, within EX31_RS17050 at 1.556 kb on - strand, within EX31_RS17050 at 1.556 kb on - strand, within EX31_RS17050 at 1.559 kb on + strand, within EX31_RS17050 at 1.560 kb on - strand, within EX31_RS17050 at 1.579 kb on + strand, within EX31_RS17050 at 1.580 kb on - strand, within EX31_RS17050 at 1.588 kb on - strand, within EX31_RS17050 at 1.595 kb on - strand, within EX31_RS17050 at 1.595 kb on - strand, within EX31_RS17050 at 1.644 kb on - strand, within EX31_RS17050 at 1.709 kb on + strand, within EX31_RS17050 at 1.709 kb on + strand, within EX31_RS17050 at 1.710 kb on - strand, within EX31_RS17050 at 1.710 kb on - strand, within EX31_RS17050 at 1.710 kb on - strand, within EX31_RS17050 at 1.816 kb on + strand at 1.816 kb on + strand at 1.816 kb on + strand at 1.817 kb on - strand at 1.817 kb on - strand at 1.817 kb on - strand at 1.817 kb on - strand at 1.817 kb on - strand at 1.817 kb on - strand at 1.817 kb on - strand at 1.817 kb on - strand at 1.817 kb on - strand at 1.823 kb on + strand at 1.824 kb on - strand at 1.857 kb on - strand at 1.857 kb on - strand at 1.857 kb on - strand at 1.866 kb on + strand at 1.866 kb on + strand at 1.866 kb on + strand at 1.867 kb on - strand at 1.867 kb on - strand at 1.907 kb on + strand, within EX31_RS17055 at 1.907 kb on + strand, within EX31_RS17055 at 1.907 kb on + strand, within EX31_RS17055 at 1.907 kb on + strand, within EX31_RS17055 at 1.907 kb on + strand, within EX31_RS17055 at 1.908 kb on - strand, within EX31_RS17055 at 1.908 kb on - strand, within EX31_RS17055 at 1.908 kb on - strand, within EX31_RS17055
Per-strain Table
Position Strand Gene LocusTag Fraction casaminos (C) remove 849 - EX31_RS26065 0.44 -2.3 849 - EX31_RS26065 0.44 +0.4 897 + EX31_RS26065 0.89 -0.5 897 + EX31_RS26065 0.89 -2.2 897 + EX31_RS26065 0.89 +1.2 898 - +0.7 898 - -2.4 898 - +0.4 898 - +0.5 908 + -1.9 909 - -1.6 909 - +0.8 909 - +0.6 909 - +1.7 959 + -1.9 959 + -1.9 984 + +0.9 984 + +0.4 984 + +0.7 984 + -1.6 985 - +0.5 985 - -1.9 985 - +1.6 989 + +0.7 989 + +1.7 992 + +2.7 992 + +0.4 993 - +0.2 993 - +0.7 993 - -0.2 1,005 + -0.6 1,006 - +0.4 1,006 - +1.0 1,006 - +0.1 1,009 + +0.4 1,010 - +1.4 1,018 + +0.4 1,018 + -1.6 1,018 + -1.6 1,018 + +0.1 1,019 - +0.2 1,019 - -0.2 1,019 - +0.7 1,027 - -0.0 1,047 + -1.6 1,070 + EX31_RS17045 0.17 -0.0 1,097 + EX31_RS17045 0.25 +0.1 1,098 - EX31_RS17045 0.26 +0.4 1,104 + EX31_RS17045 0.28 +2.2 1,104 + EX31_RS17045 0.28 +0.4 1,104 + EX31_RS17045 0.28 -0.3 1,104 + EX31_RS17045 0.28 +0.4 1,105 - EX31_RS17045 0.28 -1.9 1,105 - EX31_RS17045 0.28 -0.1 1,105 - EX31_RS17045 0.28 -1.6 1,105 - EX31_RS17045 0.28 +0.8 1,234 + EX31_RS17045 0.69 -0.0 1,234 + EX31_RS17045 0.69 -0.2 1,234 + EX31_RS17045 0.69 -0.0 1,234 + EX31_RS17045 0.69 +0.4 1,234 + EX31_RS17045 0.69 -0.6 1,234 + EX31_RS17045 0.69 +0.5 1,235 - EX31_RS17045 0.69 +0.6 1,235 - EX31_RS17045 0.69 +1.6 1,235 - EX31_RS17045 0.69 +0.1 1,235 - EX31_RS17045 0.69 +0.1 1,235 - EX31_RS17045 0.69 -0.0 1,235 - EX31_RS17045 0.69 -0.5 1,235 - EX31_RS17045 0.69 -0.2 1,235 - EX31_RS17045 0.69 -1.2 1,235 - EX31_RS17045 0.69 -1.4 1,235 - EX31_RS17045 0.69 -0.6 1,235 - EX31_RS17045 0.69 -0.6 1,266 - EX31_RS17045 0.79 -0.6 1,317 + +0.4 1,318 - +2.0 1,333 - +1.4 1,333 - +0.4 1,333 - -0.5 1,333 - +0.4 1,358 + +0.4 1,358 + -0.6 1,359 - -1.0 1,359 - +0.9 1,359 - -0.4 1,359 - -0.3 1,359 - -1.0 1,367 + +0.8 1,445 - EX31_RS17050 0.24 -0.2 1,445 - EX31_RS17050 0.24 +0.2 1,445 - EX31_RS17050 0.24 +1.0 1,445 - EX31_RS17050 0.24 -1.9 1,458 + EX31_RS17050 0.27 -0.6 1,459 - EX31_RS17050 0.27 +0.4 1,555 + EX31_RS17050 0.47 -0.6 1,556 - EX31_RS17050 0.47 +1.5 1,556 - EX31_RS17050 0.47 +0.7 1,556 - EX31_RS17050 0.47 +0.4 1,556 - EX31_RS17050 0.47 +0.1 1,556 - EX31_RS17050 0.47 -2.8 1,556 - EX31_RS17050 0.47 -0.9 1,556 - EX31_RS17050 0.47 +0.4 1,559 + EX31_RS17050 0.48 -1.0 1,560 - EX31_RS17050 0.48 +1.2 1,579 + EX31_RS17050 0.52 +1.4 1,580 - EX31_RS17050 0.53 +1.5 1,588 - EX31_RS17050 0.54 -0.6 1,595 - EX31_RS17050 0.56 +1.0 1,595 - EX31_RS17050 0.56 +0.7 1,644 - EX31_RS17050 0.66 -2.1 1,709 + EX31_RS17050 0.80 +0.0 1,709 + EX31_RS17050 0.80 +0.4 1,710 - EX31_RS17050 0.80 +0.4 1,710 - EX31_RS17050 0.80 -0.6 1,710 - EX31_RS17050 0.80 +0.0 1,816 + +0.4 1,816 + -0.0 1,816 + -0.4 1,817 - +0.9 1,817 - +0.1 1,817 - -0.7 1,817 - -0.3 1,817 - +1.4 1,817 - +0.9 1,817 - +0.1 1,817 - +2.7 1,817 - +0.1 1,823 + -1.2 1,824 - -0.4 1,857 - -1.8 1,857 - -1.4 1,857 - +0.2 1,866 + +0.4 1,866 + -0.6 1,866 + +1.0 1,867 - +1.6 1,867 - -0.3 1,907 + EX31_RS17055 0.13 -0.2 1,907 + EX31_RS17055 0.13 +0.0 1,907 + EX31_RS17055 0.13 +1.8 1,907 + EX31_RS17055 0.13 -0.2 1,907 + EX31_RS17055 0.13 -0.2 1,908 - EX31_RS17055 0.13 +0.4 1,908 - EX31_RS17055 0.13 -0.0 1,908 - EX31_RS17055 0.13 +0.1
Or see this region's nucleotide sequence