Strain Fitness in Rahnella sp. WP5 around EX31_RS23445

Experiment: casaminos (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX31_RS23440 and EX31_RS23445 are separated by 46 nucleotidesEX31_RS23445 and EX31_RS23450 are separated by 92 nucleotides EX31_RS23440: EX31_RS23440 - 2-oxoglutarate dehydrogenase E1 component, at 454,376 to 457,183 _RS23440 EX31_RS23445: EX31_RS23445 - 2-oxoglutarate dehydrogenase complex dihydrolipoyllysine-residue succinyltransferase, at 457,230 to 458,459 _RS23445 EX31_RS23450: EX31_RS23450 - ADP-forming succinate--CoA ligase subunit beta, at 458,552 to 459,718 _RS23450 Position (kb) 457 458 459Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 456.313 kb on + strand, within EX31_RS23440at 456.653 kb on + strand, within EX31_RS23440at 457.077 kb on + strandat 457.204 kb on + strandat 458.346 kb on + strandat 458.346 kb on + strandat 458.457 kb on + strandat 458.457 kb on + strandat 458.458 kb on - strandat 458.534 kb on - strandat 458.802 kb on - strand, within EX31_RS23450at 458.929 kb on + strand, within EX31_RS23450at 458.929 kb on + strand, within EX31_RS23450at 458.929 kb on + strand, within EX31_RS23450at 458.930 kb on - strand, within EX31_RS23450at 459.305 kb on + strand, within EX31_RS23450at 459.305 kb on + strand, within EX31_RS23450at 459.306 kb on - strand, within EX31_RS23450at 459.306 kb on - strand, within EX31_RS23450

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Per-strain Table

Position Strand Gene LocusTag Fraction casaminos (C)
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456,313 + EX31_RS23440 0.69 -0.8
456,653 + EX31_RS23440 0.81 +0.8
457,077 + +0.8
457,204 + -1.8
458,346 + -0.2
458,346 + -1.0
458,457 + -1.0
458,457 + -1.4
458,458 - -2.0
458,534 - -2.0
458,802 - EX31_RS23450 0.21 -0.2
458,929 + EX31_RS23450 0.32 +0.8
458,929 + EX31_RS23450 0.32 -2.9
458,929 + EX31_RS23450 0.32 -3.9
458,930 - EX31_RS23450 0.32 -0.8
459,305 + EX31_RS23450 0.65 -3.4
459,305 + EX31_RS23450 0.65 -0.8
459,306 - EX31_RS23450 0.65 -0.2
459,306 - EX31_RS23450 0.65 -2.5

Or see this region's nucleotide sequence