Experiment: casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS21955 and EX31_RS21960 are separated by 275 nucleotides EX31_RS21960 and EX31_RS21965 are separated by 548 nucleotides
EX31_RS21955: EX31_RS21955 - methyltransferase, at 135,284 to 135,862
_RS21955
EX31_RS21960: EX31_RS21960 - shikimate kinase AroL, at 136,138 to 136,662
_RS21960
EX31_RS21965: EX31_RS21965 - IclR family transcriptional regulator, at 137,211 to 137,993
_RS21965
Position (kb) 136
137 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 135.138 kb on + strand at 135.159 kb on + strand at 135.181 kb on - strand at 135.252 kb on - strand at 135.252 kb on - strand at 135.253 kb on + strand at 135.253 kb on + strand at 135.254 kb on - strand at 135.261 kb on + strand at 135.261 kb on + strand at 135.262 kb on - strand at 135.262 kb on - strand at 135.262 kb on - strand at 135.262 kb on - strand at 135.289 kb on - strand at 135.364 kb on + strand, within EX31_RS21955 at 135.364 kb on + strand, within EX31_RS21955 at 135.547 kb on - strand, within EX31_RS21955 at 135.565 kb on + strand, within EX31_RS21955 at 135.565 kb on + strand, within EX31_RS21955 at 135.565 kb on + strand, within EX31_RS21955 at 135.565 kb on + strand, within EX31_RS21955 at 135.566 kb on - strand, within EX31_RS21955 at 135.635 kb on + strand, within EX31_RS21955 at 135.636 kb on - strand, within EX31_RS21955 at 135.636 kb on - strand, within EX31_RS21955 at 135.755 kb on + strand, within EX31_RS21955 at 135.788 kb on + strand, within EX31_RS21955 at 135.788 kb on + strand, within EX31_RS21955 at 135.789 kb on - strand, within EX31_RS21955 at 135.789 kb on - strand, within EX31_RS21955 at 135.842 kb on + strand at 135.843 kb on - strand at 135.843 kb on - strand at 135.843 kb on - strand at 135.843 kb on - strand at 135.845 kb on - strand at 135.845 kb on - strand at 135.881 kb on - strand at 135.896 kb on + strand at 136.015 kb on + strand at 136.077 kb on + strand at 136.077 kb on + strand at 136.077 kb on + strand at 136.077 kb on + strand at 136.078 kb on - strand at 136.078 kb on - strand at 136.136 kb on + strand at 136.179 kb on + strand at 136.278 kb on + strand, within EX31_RS21960 at 136.278 kb on + strand, within EX31_RS21960 at 136.278 kb on + strand, within EX31_RS21960 at 136.278 kb on + strand, within EX31_RS21960 at 136.278 kb on + strand, within EX31_RS21960 at 136.279 kb on - strand, within EX31_RS21960 at 136.331 kb on + strand, within EX31_RS21960 at 136.331 kb on + strand, within EX31_RS21960 at 136.331 kb on + strand, within EX31_RS21960 at 136.331 kb on + strand, within EX31_RS21960 at 136.332 kb on - strand, within EX31_RS21960 at 136.408 kb on - strand, within EX31_RS21960 at 136.432 kb on + strand, within EX31_RS21960 at 136.452 kb on + strand, within EX31_RS21960 at 136.452 kb on + strand, within EX31_RS21960 at 136.452 kb on + strand, within EX31_RS21960 at 136.453 kb on - strand, within EX31_RS21960 at 136.565 kb on - strand, within EX31_RS21960 at 136.709 kb on + strand at 136.790 kb on - strand at 136.795 kb on + strand at 136.795 kb on + strand at 136.795 kb on + strand at 136.795 kb on + strand at 136.795 kb on + strand at 136.795 kb on + strand at 136.795 kb on + strand at 136.796 kb on - strand at 136.796 kb on - strand at 136.796 kb on - strand at 136.796 kb on - strand at 136.796 kb on - strand at 136.796 kb on - strand at 136.799 kb on + strand at 136.799 kb on + strand at 136.799 kb on + strand at 136.799 kb on + strand at 136.800 kb on - strand at 136.800 kb on - strand at 136.804 kb on + strand at 136.804 kb on + strand at 136.804 kb on + strand at 136.805 kb on - strand at 136.805 kb on - strand at 136.812 kb on + strand at 136.812 kb on + strand at 136.812 kb on + strand at 136.812 kb on + strand at 136.812 kb on + strand at 136.812 kb on + strand at 136.812 kb on + strand at 136.812 kb on + strand at 136.813 kb on - strand at 136.813 kb on - strand at 136.813 kb on - strand at 136.813 kb on - strand at 136.813 kb on - strand at 136.813 kb on - strand at 136.813 kb on - strand at 136.813 kb on - strand at 136.813 kb on - strand at 136.814 kb on + strand at 136.814 kb on + strand at 136.814 kb on + strand at 136.814 kb on + strand at 136.815 kb on - strand at 136.815 kb on - strand at 136.815 kb on - strand at 136.815 kb on - strand at 136.815 kb on - strand at 136.829 kb on + strand at 136.829 kb on + strand at 136.829 kb on + strand at 136.829 kb on + strand at 136.830 kb on - strand at 136.830 kb on - strand at 136.830 kb on - strand at 136.830 kb on - strand at 136.832 kb on + strand at 136.833 kb on - strand at 136.833 kb on - strand at 136.897 kb on + strand at 136.973 kb on - strand at 137.012 kb on + strand at 137.029 kb on + strand at 137.030 kb on - strand at 137.030 kb on - strand at 137.030 kb on - strand at 137.030 kb on - strand at 137.030 kb on - strand at 137.030 kb on - strand at 137.030 kb on - strand at 137.033 kb on - strand at 137.033 kb on - strand at 137.033 kb on - strand at 137.043 kb on + strand at 137.043 kb on + strand at 137.044 kb on - strand at 137.060 kb on - strand at 137.060 kb on - strand at 137.069 kb on + strand at 137.069 kb on + strand at 137.070 kb on - strand at 137.070 kb on - strand at 137.073 kb on + strand at 137.091 kb on + strand at 137.091 kb on + strand at 137.092 kb on - strand at 137.092 kb on - strand at 137.092 kb on - strand at 137.092 kb on - strand at 137.146 kb on + strand at 137.147 kb on - strand at 137.163 kb on - strand at 137.171 kb on + strand at 137.190 kb on + strand at 137.221 kb on + strand at 137.221 kb on + strand at 137.221 kb on + strand at 137.268 kb on - strand at 137.330 kb on + strand, within EX31_RS21965 at 137.330 kb on + strand, within EX31_RS21965 at 137.331 kb on - strand, within EX31_RS21965 at 137.331 kb on - strand, within EX31_RS21965 at 137.401 kb on - strand, within EX31_RS21965 at 137.416 kb on + strand, within EX31_RS21965 at 137.419 kb on + strand, within EX31_RS21965 at 137.439 kb on + strand, within EX31_RS21965 at 137.439 kb on + strand, within EX31_RS21965 at 137.464 kb on + strand, within EX31_RS21965 at 137.475 kb on + strand, within EX31_RS21965 at 137.475 kb on + strand, within EX31_RS21965 at 137.475 kb on + strand, within EX31_RS21965 at 137.475 kb on + strand, within EX31_RS21965 at 137.475 kb on + strand, within EX31_RS21965 at 137.475 kb on + strand, within EX31_RS21965 at 137.476 kb on - strand, within EX31_RS21965 at 137.476 kb on - strand, within EX31_RS21965 at 137.477 kb on + strand, within EX31_RS21965 at 137.477 kb on + strand, within EX31_RS21965 at 137.477 kb on + strand, within EX31_RS21965 at 137.477 kb on + strand, within EX31_RS21965 at 137.478 kb on - strand, within EX31_RS21965 at 137.478 kb on - strand, within EX31_RS21965 at 137.478 kb on - strand, within EX31_RS21965 at 137.478 kb on - strand, within EX31_RS21965 at 137.478 kb on - strand, within EX31_RS21965 at 137.515 kb on - strand, within EX31_RS21965 at 137.515 kb on - strand, within EX31_RS21965 at 137.516 kb on + strand, within EX31_RS21965 at 137.516 kb on + strand, within EX31_RS21965 at 137.516 kb on + strand, within EX31_RS21965 at 137.516 kb on + strand, within EX31_RS21965 at 137.517 kb on - strand, within EX31_RS21965 at 137.517 kb on - strand, within EX31_RS21965 at 137.543 kb on + strand, within EX31_RS21965 at 137.543 kb on + strand, within EX31_RS21965 at 137.544 kb on - strand, within EX31_RS21965 at 137.544 kb on - strand, within EX31_RS21965 at 137.574 kb on + strand, within EX31_RS21965 at 137.574 kb on + strand, within EX31_RS21965 at 137.574 kb on + strand, within EX31_RS21965 at 137.575 kb on - strand, within EX31_RS21965 at 137.575 kb on - strand, within EX31_RS21965 at 137.575 kb on - strand, within EX31_RS21965 at 137.575 kb on - strand, within EX31_RS21965 at 137.588 kb on + strand, within EX31_RS21965 at 137.652 kb on + strand, within EX31_RS21965 at 137.652 kb on + strand, within EX31_RS21965
Per-strain Table
Position Strand Gene LocusTag Fraction casaminos (C) remove 135,138 + -0.4 135,159 + -1.8 135,181 - -1.8 135,252 - +1.2 135,252 - +0.6 135,253 + -1.1 135,253 + -0.5 135,254 - -0.5 135,261 + +0.4 135,261 + +0.6 135,262 - +0.0 135,262 - +1.3 135,262 - -0.2 135,262 - -0.5 135,289 - -0.8 135,364 + EX31_RS21955 0.14 +0.7 135,364 + EX31_RS21955 0.14 +0.1 135,547 - EX31_RS21955 0.45 +0.8 135,565 + EX31_RS21955 0.49 -0.3 135,565 + EX31_RS21955 0.49 +2.0 135,565 + EX31_RS21955 0.49 +2.6 135,565 + EX31_RS21955 0.49 -0.7 135,566 - EX31_RS21955 0.49 -2.2 135,635 + EX31_RS21955 0.61 -0.6 135,636 - EX31_RS21955 0.61 -0.7 135,636 - EX31_RS21955 0.61 +1.4 135,755 + EX31_RS21955 0.81 +0.0 135,788 + EX31_RS21955 0.87 +3.0 135,788 + EX31_RS21955 0.87 +1.5 135,789 - EX31_RS21955 0.87 +0.1 135,789 - EX31_RS21955 0.87 +2.0 135,842 + +1.5 135,843 - -0.4 135,843 - -1.0 135,843 - +2.2 135,843 - -0.3 135,845 - +1.2 135,845 - +0.6 135,881 - -2.0 135,896 + -0.2 136,015 + -0.7 136,077 + -1.8 136,077 + +0.0 136,077 + -1.1 136,077 + +0.0 136,078 - +0.6 136,078 - +0.6 136,136 + +0.2 136,179 + +0.6 136,278 + EX31_RS21960 0.27 -1.0 136,278 + EX31_RS21960 0.27 +0.0 136,278 + EX31_RS21960 0.27 -1.3 136,278 + EX31_RS21960 0.27 -0.8 136,278 + EX31_RS21960 0.27 -1.0 136,279 - EX31_RS21960 0.27 -0.4 136,331 + EX31_RS21960 0.37 -0.8 136,331 + EX31_RS21960 0.37 +1.0 136,331 + EX31_RS21960 0.37 -1.4 136,331 + EX31_RS21960 0.37 +0.2 136,332 - EX31_RS21960 0.37 -0.3 136,408 - EX31_RS21960 0.51 -1.6 136,432 + EX31_RS21960 0.56 +0.3 136,452 + EX31_RS21960 0.60 -1.3 136,452 + EX31_RS21960 0.60 -0.6 136,452 + EX31_RS21960 0.60 +0.9 136,453 - EX31_RS21960 0.60 -0.4 136,565 - EX31_RS21960 0.81 -2.0 136,709 + +0.5 136,790 - +1.3 136,795 + +1.6 136,795 + +0.0 136,795 + +0.4 136,795 + -1.6 136,795 + +0.0 136,795 + +0.3 136,795 + -0.3 136,796 - -1.0 136,796 - -0.7 136,796 - +2.0 136,796 - -1.2 136,796 - +1.0 136,796 - -0.0 136,799 + -1.4 136,799 + +0.1 136,799 + +0.4 136,799 + +1.4 136,800 - +0.0 136,800 - +3.0 136,804 + +0.5 136,804 + -0.2 136,804 + -0.7 136,805 - +0.3 136,805 - +0.0 136,812 + -0.4 136,812 + +1.0 136,812 + +1.7 136,812 + -2.2 136,812 + -0.9 136,812 + -1.0 136,812 + +0.1 136,812 + +0.7 136,813 - +2.0 136,813 - +0.3 136,813 - +0.7 136,813 - +0.3 136,813 - +0.3 136,813 - -0.5 136,813 - +0.4 136,813 - +0.4 136,813 - +1.0 136,814 + -1.3 136,814 + +1.0 136,814 + +2.0 136,814 + +1.5 136,815 - -0.1 136,815 - +0.0 136,815 - +1.0 136,815 - -0.1 136,815 - +0.4 136,829 + +1.0 136,829 + +0.6 136,829 + +0.7 136,829 + +0.5 136,830 - +0.9 136,830 - +0.6 136,830 - -1.3 136,830 - +0.8 136,832 + +0.4 136,833 - -0.4 136,833 - +1.9 136,897 + -0.4 136,973 - +0.1 137,012 + +0.4 137,029 + +0.0 137,030 - -1.1 137,030 - +0.1 137,030 - -0.6 137,030 - -0.8 137,030 - -1.3 137,030 - +0.7 137,030 - +0.6 137,033 - +0.3 137,033 - +2.3 137,033 - +0.2 137,043 + +2.2 137,043 + -0.3 137,044 - +0.2 137,060 - +0.1 137,060 - -0.6 137,069 + -0.7 137,069 + +0.5 137,070 - -0.8 137,070 - -0.6 137,073 + -0.6 137,091 + -0.8 137,091 + -0.3 137,092 - +0.0 137,092 - +0.7 137,092 - +0.8 137,092 - +1.7 137,146 + +0.4 137,147 - +0.4 137,163 - +1.4 137,171 + -0.4 137,190 + +0.7 137,221 + +0.4 137,221 + +1.0 137,221 + +2.3 137,268 - +0.6 137,330 + EX31_RS21965 0.15 +0.0 137,330 + EX31_RS21965 0.15 +1.0 137,331 - EX31_RS21965 0.15 +0.0 137,331 - EX31_RS21965 0.15 -1.0 137,401 - EX31_RS21965 0.24 +1.9 137,416 + EX31_RS21965 0.26 +1.0 137,419 + EX31_RS21965 0.27 -0.7 137,439 + EX31_RS21965 0.29 +0.6 137,439 + EX31_RS21965 0.29 +0.5 137,464 + EX31_RS21965 0.32 -1.3 137,475 + EX31_RS21965 0.34 -1.0 137,475 + EX31_RS21965 0.34 +0.0 137,475 + EX31_RS21965 0.34 -0.8 137,475 + EX31_RS21965 0.34 +1.7 137,475 + EX31_RS21965 0.34 +0.6 137,475 + EX31_RS21965 0.34 +0.6 137,476 - EX31_RS21965 0.34 -0.3 137,476 - EX31_RS21965 0.34 +1.0 137,477 + EX31_RS21965 0.34 +0.0 137,477 + EX31_RS21965 0.34 +0.4 137,477 + EX31_RS21965 0.34 -0.3 137,477 + EX31_RS21965 0.34 -2.6 137,478 - EX31_RS21965 0.34 +0.7 137,478 - EX31_RS21965 0.34 -0.7 137,478 - EX31_RS21965 0.34 +1.0 137,478 - EX31_RS21965 0.34 +0.6 137,478 - EX31_RS21965 0.34 -2.1 137,515 - EX31_RS21965 0.39 +0.5 137,515 - EX31_RS21965 0.39 +0.6 137,516 + EX31_RS21965 0.39 -0.2 137,516 + EX31_RS21965 0.39 +1.4 137,516 + EX31_RS21965 0.39 +1.1 137,516 + EX31_RS21965 0.39 +0.6 137,517 - EX31_RS21965 0.39 +0.7 137,517 - EX31_RS21965 0.39 +0.0 137,543 + EX31_RS21965 0.42 -0.7 137,543 + EX31_RS21965 0.42 +0.3 137,544 - EX31_RS21965 0.43 +0.0 137,544 - EX31_RS21965 0.43 +0.7 137,574 + EX31_RS21965 0.46 +1.4 137,574 + EX31_RS21965 0.46 +1.1 137,574 + EX31_RS21965 0.46 +2.4 137,575 - EX31_RS21965 0.46 +1.0 137,575 - EX31_RS21965 0.46 -0.3 137,575 - EX31_RS21965 0.46 +1.7 137,575 - EX31_RS21965 0.46 +0.6 137,588 + EX31_RS21965 0.48 +0.3 137,652 + EX31_RS21965 0.56 +0.1 137,652 + EX31_RS21965 0.56 +0.7
Or see this region's nucleotide sequence