Experiment: casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS18435 and EX31_RS18440 are separated by 2 nucleotides EX31_RS18440 and EX31_RS18445 overlap by 1 nucleotides EX31_RS18445 and EX31_RS18450 are separated by 59 nucleotides EX31_RS18450 and EX31_RS18455 are separated by 178 nucleotides EX31_RS18455 and EX31_RS18460 are separated by 100 nucleotides
EX31_RS18435: EX31_RS18435 - DUF5405 family protein, at 260,565 to 260,846
_RS18435
EX31_RS18440: EX31_RS18440 - TraR/DksA family transcriptional regulator, at 260,849 to 261,067
_RS18440
EX31_RS18445: EX31_RS18445 - DUF2732 domain-containing protein, at 261,067 to 261,309
_RS18445
EX31_RS18450: EX31_RS18450 - hypothetical protein, at 261,369 to 261,869
_RS18450
EX31_RS18455: EX31_RS18455 - hypothetical protein, at 262,048 to 262,419
_RS18455
EX31_RS18460: EX31_RS18460 - helix-turn-helix transcriptional regulator, at 262,520 to 262,819
_RS18460
Position (kb) 261
262 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 260.385 kb on - strand at 260.407 kb on + strand at 260.407 kb on + strand at 260.489 kb on + strand at 260.490 kb on - strand at 260.490 kb on - strand at 260.574 kb on - strand at 260.582 kb on + strand at 260.583 kb on - strand at 260.653 kb on - strand, within EX31_RS18435 at 260.698 kb on - strand, within EX31_RS18435 at 260.698 kb on - strand, within EX31_RS18435 at 260.719 kb on + strand, within EX31_RS18435 at 260.719 kb on + strand, within EX31_RS18435 at 260.719 kb on + strand, within EX31_RS18435 at 260.719 kb on + strand, within EX31_RS18435 at 260.720 kb on - strand, within EX31_RS18435 at 260.720 kb on - strand, within EX31_RS18435 at 260.720 kb on - strand, within EX31_RS18435 at 260.720 kb on - strand, within EX31_RS18435 at 260.733 kb on + strand, within EX31_RS18435 at 260.734 kb on - strand, within EX31_RS18435 at 260.734 kb on - strand, within EX31_RS18435 at 260.738 kb on - strand, within EX31_RS18435 at 260.812 kb on - strand, within EX31_RS18435 at 260.850 kb on + strand at 260.850 kb on + strand at 260.989 kb on + strand, within EX31_RS18440 at 260.990 kb on - strand, within EX31_RS18440 at 260.990 kb on - strand, within EX31_RS18440 at 260.995 kb on + strand, within EX31_RS18440 at 260.995 kb on + strand, within EX31_RS18440 at 260.996 kb on - strand, within EX31_RS18440 at 261.008 kb on + strand, within EX31_RS18440 at 261.008 kb on + strand, within EX31_RS18440 at 261.068 kb on + strand at 261.069 kb on - strand at 261.069 kb on - strand at 261.069 kb on - strand at 261.128 kb on - strand, within EX31_RS18445 at 261.174 kb on + strand, within EX31_RS18445 at 261.179 kb on - strand, within EX31_RS18445 at 261.245 kb on - strand, within EX31_RS18445 at 261.370 kb on + strand at 261.371 kb on - strand at 261.379 kb on + strand at 261.402 kb on + strand at 261.406 kb on - strand at 261.567 kb on + strand, within EX31_RS18450 at 261.606 kb on - strand, within EX31_RS18450 at 261.654 kb on - strand, within EX31_RS18450 at 261.763 kb on + strand, within EX31_RS18450 at 261.844 kb on - strand at 261.844 kb on - strand at 261.885 kb on - strand at 261.896 kb on + strand at 261.897 kb on - strand at 261.913 kb on + strand at 261.913 kb on + strand at 261.932 kb on - strand at 261.961 kb on + strand at 261.962 kb on - strand at 261.962 kb on - strand at 261.970 kb on + strand at 261.971 kb on - strand at 262.033 kb on + strand at 262.033 kb on + strand at 262.034 kb on - strand at 262.035 kb on + strand at 262.035 kb on + strand at 262.035 kb on + strand at 262.036 kb on - strand at 262.036 kb on - strand at 262.036 kb on - strand at 262.036 kb on - strand at 262.036 kb on - strand at 262.049 kb on + strand at 262.049 kb on + strand at 262.049 kb on + strand at 262.050 kb on - strand at 262.050 kb on - strand at 262.050 kb on - strand at 262.050 kb on - strand at 262.050 kb on - strand at 262.080 kb on + strand at 262.081 kb on - strand at 262.081 kb on - strand at 262.081 kb on - strand at 262.089 kb on + strand, within EX31_RS18455 at 262.093 kb on - strand, within EX31_RS18455 at 262.178 kb on - strand, within EX31_RS18455 at 262.217 kb on + strand, within EX31_RS18455 at 262.217 kb on + strand, within EX31_RS18455 at 262.218 kb on - strand, within EX31_RS18455 at 262.218 kb on - strand, within EX31_RS18455 at 262.218 kb on - strand, within EX31_RS18455 at 262.218 kb on - strand, within EX31_RS18455 at 262.252 kb on + strand, within EX31_RS18455 at 262.253 kb on - strand, within EX31_RS18455 at 262.273 kb on - strand, within EX31_RS18455 at 262.273 kb on - strand, within EX31_RS18455 at 262.273 kb on - strand, within EX31_RS18455 at 262.276 kb on + strand, within EX31_RS18455 at 262.276 kb on + strand, within EX31_RS18455 at 262.276 kb on + strand, within EX31_RS18455 at 262.276 kb on + strand, within EX31_RS18455 at 262.277 kb on - strand, within EX31_RS18455 at 262.277 kb on - strand, within EX31_RS18455 at 262.277 kb on - strand, within EX31_RS18455 at 262.277 kb on - strand, within EX31_RS18455 at 262.277 kb on - strand, within EX31_RS18455 at 262.277 kb on - strand, within EX31_RS18455 at 262.277 kb on - strand, within EX31_RS18455 at 262.280 kb on + strand, within EX31_RS18455 at 262.280 kb on + strand, within EX31_RS18455 at 262.280 kb on + strand, within EX31_RS18455 at 262.280 kb on + strand, within EX31_RS18455 at 262.280 kb on + strand, within EX31_RS18455 at 262.280 kb on + strand, within EX31_RS18455 at 262.281 kb on - strand, within EX31_RS18455 at 262.281 kb on - strand, within EX31_RS18455 at 262.281 kb on - strand, within EX31_RS18455 at 262.281 kb on - strand, within EX31_RS18455 at 262.341 kb on + strand, within EX31_RS18455 at 262.342 kb on - strand, within EX31_RS18455 at 262.342 kb on - strand, within EX31_RS18455 at 262.342 kb on - strand, within EX31_RS18455 at 262.342 kb on - strand, within EX31_RS18455 at 262.468 kb on + strand at 262.823 kb on + strand at 262.825 kb on + strand at 262.825 kb on + strand at 262.825 kb on + strand at 262.825 kb on + strand at 262.825 kb on + strand at 262.825 kb on + strand at 262.825 kb on + strand at 262.825 kb on + strand at 262.825 kb on + strand at 262.825 kb on + strand at 262.825 kb on + strand at 262.825 kb on + strand at 262.825 kb on + strand at 262.825 kb on + strand at 262.825 kb on + strand at 262.825 kb on + strand at 262.825 kb on + strand at 262.825 kb on + strand at 262.825 kb on + strand at 262.825 kb on + strand at 262.825 kb on + strand at 262.826 kb on - strand at 262.826 kb on - strand at 262.826 kb on - strand at 262.826 kb on - strand at 262.826 kb on - strand at 262.838 kb on + strand at 262.838 kb on + strand at 262.838 kb on + strand at 262.839 kb on - strand at 262.839 kb on - strand at 262.839 kb on - strand at 262.867 kb on + strand at 262.867 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction casaminos (C) remove 260,385 - +0.8 260,407 + -1.8 260,407 + -0.2 260,489 + +0.9 260,490 - +1.2 260,490 - -0.5 260,574 - -0.3 260,582 + -3.0 260,583 - -0.6 260,653 - EX31_RS18435 0.31 -0.0 260,698 - EX31_RS18435 0.47 -1.9 260,698 - EX31_RS18435 0.47 -0.9 260,719 + EX31_RS18435 0.55 +2.1 260,719 + EX31_RS18435 0.55 +1.8 260,719 + EX31_RS18435 0.55 -0.3 260,719 + EX31_RS18435 0.55 -1.8 260,720 - EX31_RS18435 0.55 -0.2 260,720 - EX31_RS18435 0.55 -0.2 260,720 - EX31_RS18435 0.55 +2.4 260,720 - EX31_RS18435 0.55 -0.2 260,733 + EX31_RS18435 0.60 +0.3 260,734 - EX31_RS18435 0.60 -0.5 260,734 - EX31_RS18435 0.60 -1.3 260,738 - EX31_RS18435 0.61 -0.0 260,812 - EX31_RS18435 0.88 -1.5 260,850 + -0.2 260,850 + +1.4 260,989 + EX31_RS18440 0.64 -2.2 260,990 - EX31_RS18440 0.64 -0.8 260,990 - EX31_RS18440 0.64 -0.5 260,995 + EX31_RS18440 0.67 +0.7 260,995 + EX31_RS18440 0.67 -0.6 260,996 - EX31_RS18440 0.67 -0.6 261,008 + EX31_RS18440 0.73 +0.5 261,008 + EX31_RS18440 0.73 +0.0 261,068 + -0.8 261,069 - -0.8 261,069 - -1.5 261,069 - -0.5 261,128 - EX31_RS18445 0.25 +0.5 261,174 + EX31_RS18445 0.44 +3.6 261,179 - EX31_RS18445 0.46 -1.8 261,245 - EX31_RS18445 0.73 +1.0 261,370 + +0.8 261,371 - -0.2 261,379 + +1.5 261,402 + -1.2 261,406 - +1.6 261,567 + EX31_RS18450 0.40 +0.8 261,606 - EX31_RS18450 0.47 -1.0 261,654 - EX31_RS18450 0.57 -0.2 261,763 + EX31_RS18450 0.79 -0.2 261,844 - -1.0 261,844 - -0.1 261,885 - -2.8 261,896 + -0.4 261,897 - +3.8 261,913 + +0.2 261,913 + +0.1 261,932 - +0.0 261,961 + -0.2 261,962 - -0.1 261,962 - +0.2 261,970 + -1.8 261,971 - +0.4 262,033 + -0.2 262,033 + -0.6 262,034 - +0.4 262,035 + +0.2 262,035 + -0.0 262,035 + +1.8 262,036 - +0.8 262,036 - +1.7 262,036 - -1.2 262,036 - +0.5 262,036 - -0.2 262,049 + -1.2 262,049 + +1.4 262,049 + -0.9 262,050 - -0.0 262,050 - +0.0 262,050 - -0.9 262,050 - -0.2 262,050 - +0.2 262,080 + +0.8 262,081 - +0.5 262,081 - -0.4 262,081 - +1.1 262,089 + EX31_RS18455 0.11 +0.8 262,093 - EX31_RS18455 0.12 +1.8 262,178 - EX31_RS18455 0.35 +1.8 262,217 + EX31_RS18455 0.45 +1.4 262,217 + EX31_RS18455 0.45 -0.5 262,218 - EX31_RS18455 0.46 -0.3 262,218 - EX31_RS18455 0.46 -0.2 262,218 - EX31_RS18455 0.46 -1.2 262,218 - EX31_RS18455 0.46 +1.2 262,252 + EX31_RS18455 0.55 +0.8 262,253 - EX31_RS18455 0.55 +0.8 262,273 - EX31_RS18455 0.60 -0.3 262,273 - EX31_RS18455 0.60 +1.4 262,273 - EX31_RS18455 0.60 +0.8 262,276 + EX31_RS18455 0.61 +0.2 262,276 + EX31_RS18455 0.61 -0.2 262,276 + EX31_RS18455 0.61 +1.4 262,276 + EX31_RS18455 0.61 -2.2 262,277 - EX31_RS18455 0.62 +1.4 262,277 - EX31_RS18455 0.62 +1.1 262,277 - EX31_RS18455 0.62 +1.1 262,277 - EX31_RS18455 0.62 +1.8 262,277 - EX31_RS18455 0.62 -1.8 262,277 - EX31_RS18455 0.62 +1.0 262,277 - EX31_RS18455 0.62 +0.8 262,280 + EX31_RS18455 0.62 -0.1 262,280 + EX31_RS18455 0.62 +0.2 262,280 + EX31_RS18455 0.62 +1.7 262,280 + EX31_RS18455 0.62 +0.5 262,280 + EX31_RS18455 0.62 -0.6 262,280 + EX31_RS18455 0.62 +1.1 262,281 - EX31_RS18455 0.63 +1.1 262,281 - EX31_RS18455 0.63 -1.5 262,281 - EX31_RS18455 0.63 +1.3 262,281 - EX31_RS18455 0.63 +1.2 262,341 + EX31_RS18455 0.79 -0.5 262,342 - EX31_RS18455 0.79 -0.7 262,342 - EX31_RS18455 0.79 +1.8 262,342 - EX31_RS18455 0.79 -0.6 262,342 - EX31_RS18455 0.79 +0.6 262,468 + +0.7 262,823 + +1.6 262,825 + +2.6 262,825 + -0.4 262,825 + -0.5 262,825 + +0.3 262,825 + +1.2 262,825 + +0.8 262,825 + +0.4 262,825 + +0.2 262,825 + +0.1 262,825 + +1.3 262,825 + -0.4 262,825 + +1.2 262,825 + +0.3 262,825 + +0.5 262,825 + +1.4 262,825 + +0.8 262,825 + +1.1 262,825 + +0.7 262,825 + -1.0 262,825 + -0.3 262,825 + +0.8 262,826 - +1.8 262,826 - -1.0 262,826 - -0.4 262,826 - +1.5 262,826 - -2.0 262,838 + +0.8 262,838 + +0.4 262,838 + +1.0 262,839 - +0.2 262,839 - -1.2 262,839 - +0.0 262,867 + -0.9 262,867 + -0.8
Or see this region's nucleotide sequence