Strain Fitness in Rahnella sp. WP5 around EX31_RS16720

Experiment: casaminos (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX31_RS16715 and EX31_RS16720 are separated by 261 nucleotidesEX31_RS16720 and EX31_RS16725 are separated by 161 nucleotides EX31_RS16715: EX31_RS16715 - ATP-binding cassette domain-containing protein, at 25,436 to 26,530 _RS16715 EX31_RS16720: EX31_RS16720 - helix-turn-helix domain-containing protein, at 26,792 to 27,640 _RS16720 EX31_RS16725: EX31_RS16725 - phytanoyl-CoA dioxygenase, at 27,802 to 28,569 _RS16725 Position (kb) 26 27 28Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 25.798 kb on + strand, within EX31_RS16715at 25.856 kb on - strand, within EX31_RS16715at 25.856 kb on - strand, within EX31_RS16715at 25.856 kb on - strand, within EX31_RS16715at 25.856 kb on - strand, within EX31_RS16715at 25.961 kb on - strand, within EX31_RS16715at 25.964 kb on - strand, within EX31_RS16715at 25.964 kb on - strand, within EX31_RS16715at 26.177 kb on + strand, within EX31_RS16715at 26.292 kb on + strand, within EX31_RS16715at 26.292 kb on + strand, within EX31_RS16715at 26.298 kb on - strand, within EX31_RS16715at 26.438 kb on - strandat 26.441 kb on - strandat 26.441 kb on - strandat 26.441 kb on - strandat 26.441 kb on - strandat 26.441 kb on - strandat 26.511 kb on - strandat 26.604 kb on - strandat 26.613 kb on + strandat 26.884 kb on - strand, within EX31_RS16720at 26.956 kb on + strand, within EX31_RS16720at 27.103 kb on + strand, within EX31_RS16720at 27.104 kb on - strand, within EX31_RS16720at 27.104 kb on - strand, within EX31_RS16720at 27.106 kb on - strand, within EX31_RS16720at 27.186 kb on - strand, within EX31_RS16720at 27.186 kb on - strand, within EX31_RS16720at 27.273 kb on - strand, within EX31_RS16720at 27.433 kb on + strand, within EX31_RS16720at 27.434 kb on - strand, within EX31_RS16720at 27.435 kb on + strand, within EX31_RS16720at 27.435 kb on + strand, within EX31_RS16720at 27.436 kb on - strand, within EX31_RS16720at 27.436 kb on - strand, within EX31_RS16720at 27.436 kb on - strand, within EX31_RS16720at 27.677 kb on - strandat 27.677 kb on - strandat 27.738 kb on - strandat 27.870 kb on + strandat 27.870 kb on + strandat 27.871 kb on - strandat 27.871 kb on - strandat 27.908 kb on + strand, within EX31_RS16725at 27.909 kb on - strand, within EX31_RS16725at 28.273 kb on - strand, within EX31_RS16725at 28.471 kb on - strand, within EX31_RS16725at 28.517 kb on - strandat 28.548 kb on - strandat 28.602 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction casaminos (C)
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25,798 + EX31_RS16715 0.33 -0.5
25,856 - EX31_RS16715 0.38 -0.5
25,856 - EX31_RS16715 0.38 +0.2
25,856 - EX31_RS16715 0.38 +1.7
25,856 - EX31_RS16715 0.38 -0.1
25,961 - EX31_RS16715 0.48 -0.5
25,964 - EX31_RS16715 0.48 -1.8
25,964 - EX31_RS16715 0.48 +2.6
26,177 + EX31_RS16715 0.68 +0.1
26,292 + EX31_RS16715 0.78 +1.1
26,292 + EX31_RS16715 0.78 +0.5
26,298 - EX31_RS16715 0.79 -0.4
26,438 - +1.5
26,441 - +0.5
26,441 - -1.8
26,441 - +2.3
26,441 - +2.2
26,441 - +1.0
26,511 - +0.3
26,604 - +2.1
26,613 + +0.8
26,884 - EX31_RS16720 0.11 -0.7
26,956 + EX31_RS16720 0.19 +2.5
27,103 + EX31_RS16720 0.37 +1.5
27,104 - EX31_RS16720 0.37 -2.5
27,104 - EX31_RS16720 0.37 -2.3
27,106 - EX31_RS16720 0.37 -0.8
27,186 - EX31_RS16720 0.46 -3.0
27,186 - EX31_RS16720 0.46 -0.5
27,273 - EX31_RS16720 0.57 -1.1
27,433 + EX31_RS16720 0.76 +1.8
27,434 - EX31_RS16720 0.76 -0.2
27,435 + EX31_RS16720 0.76 -0.1
27,435 + EX31_RS16720 0.76 -0.7
27,436 - EX31_RS16720 0.76 -1.1
27,436 - EX31_RS16720 0.76 -0.2
27,436 - EX31_RS16720 0.76 +0.5
27,677 - +0.5
27,677 - -0.4
27,738 - +0.2
27,870 + -1.1
27,870 + +1.1
27,871 - -0.7
27,871 - +0.5
27,908 + EX31_RS16725 0.14 +1.7
27,909 - EX31_RS16725 0.14 -1.1
28,273 - EX31_RS16725 0.61 +0.5
28,471 - EX31_RS16725 0.87 +1.2
28,517 - -0.5
28,548 - +1.9
28,602 + +1.0

Or see this region's nucleotide sequence