Experiment: casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS16620 and EX31_RS16625 are separated by 192 nucleotides EX31_RS16625 and EX31_RS16630 are separated by 27 nucleotides EX31_RS16630 and EX31_RS16635 are separated by 16 nucleotides
EX31_RS16620: EX31_RS16620 - glycosyltransferase, at 6,098 to 7,258
_RS16620
EX31_RS16625: EX31_RS16625 - bifunctional 4-hydroxy-2-oxoglutarate aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase, at 7,451 to 8,089
_RS16625
EX31_RS16630: EX31_RS16630 - phosphogluconate dehydratase, at 8,117 to 9,931
_RS16630
EX31_RS16635: EX31_RS16635 - gluconate transporter, at 9,948 to 11,285
_RS16635
Position (kb)
8
9
10 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 7.217 kb on + strand at 7.281 kb on + strand at 7.328 kb on - strand at 7.328 kb on - strand at 7.328 kb on - strand at 7.328 kb on - strand at 7.337 kb on - strand at 7.337 kb on - strand at 7.408 kb on - strand at 7.429 kb on + strand at 7.432 kb on + strand at 7.452 kb on + strand at 7.489 kb on + strand at 7.490 kb on - strand at 7.493 kb on - strand at 7.493 kb on - strand at 7.576 kb on - strand, within EX31_RS16625 at 8.126 kb on - strand at 8.438 kb on + strand, within EX31_RS16630 at 8.438 kb on + strand, within EX31_RS16630 at 8.465 kb on - strand, within EX31_RS16630 at 8.465 kb on - strand, within EX31_RS16630 at 8.831 kb on + strand, within EX31_RS16630 at 8.832 kb on - strand, within EX31_RS16630 at 8.832 kb on - strand, within EX31_RS16630 at 8.832 kb on - strand, within EX31_RS16630 at 8.900 kb on - strand, within EX31_RS16630 at 8.924 kb on - strand, within EX31_RS16630 at 8.924 kb on - strand, within EX31_RS16630 at 9.105 kb on + strand, within EX31_RS16630 at 9.106 kb on - strand, within EX31_RS16630 at 9.106 kb on - strand, within EX31_RS16630 at 9.339 kb on + strand, within EX31_RS16630 at 9.340 kb on - strand, within EX31_RS16630 at 9.447 kb on - strand, within EX31_RS16630 at 9.473 kb on - strand, within EX31_RS16630 at 9.473 kb on - strand, within EX31_RS16630 at 9.481 kb on - strand, within EX31_RS16630 at 9.719 kb on - strand, within EX31_RS16630 at 9.742 kb on + strand, within EX31_RS16630 at 9.932 kb on - strand at 9.932 kb on - strand at 9.932 kb on - strand at 9.932 kb on - strand at 10.086 kb on + strand, within EX31_RS16635 at 10.180 kb on - strand, within EX31_RS16635 at 10.234 kb on - strand, within EX31_RS16635 at 10.234 kb on - strand, within EX31_RS16635 at 10.234 kb on - strand, within EX31_RS16635 at 10.342 kb on - strand, within EX31_RS16635 at 10.484 kb on + strand, within EX31_RS16635 at 10.622 kb on + strand, within EX31_RS16635 at 10.623 kb on - strand, within EX31_RS16635 at 10.685 kb on + strand, within EX31_RS16635
Per-strain Table
Position Strand Gene LocusTag Fraction casaminos (C) remove 7,217 + +1.4 7,281 + +1.1 7,328 - +1.5 7,328 - -0.4 7,328 - +1.8 7,328 - +0.6 7,337 - -0.2 7,337 - +1.1 7,408 - +0.5 7,429 + -0.5 7,432 + -0.9 7,452 + +3.5 7,489 + +2.1 7,490 - +0.8 7,493 - +1.7 7,493 - -0.5 7,576 - EX31_RS16625 0.20 -0.3 8,126 - -1.3 8,438 + EX31_RS16630 0.18 +0.5 8,438 + EX31_RS16630 0.18 -1.2 8,465 - EX31_RS16630 0.19 +0.9 8,465 - EX31_RS16630 0.19 +0.5 8,831 + EX31_RS16630 0.39 +0.5 8,832 - EX31_RS16630 0.39 +0.5 8,832 - EX31_RS16630 0.39 -1.1 8,832 - EX31_RS16630 0.39 +1.1 8,900 - EX31_RS16630 0.43 -0.5 8,924 - EX31_RS16630 0.44 +1.1 8,924 - EX31_RS16630 0.44 -0.1 9,105 + EX31_RS16630 0.54 +2.1 9,106 - EX31_RS16630 0.54 +0.3 9,106 - EX31_RS16630 0.54 +0.2 9,339 + EX31_RS16630 0.67 +0.9 9,340 - EX31_RS16630 0.67 +0.8 9,447 - EX31_RS16630 0.73 +0.8 9,473 - EX31_RS16630 0.75 -1.4 9,473 - EX31_RS16630 0.75 +0.8 9,481 - EX31_RS16630 0.75 -0.5 9,719 - EX31_RS16630 0.88 +0.9 9,742 + EX31_RS16630 0.90 +2.1 9,932 - -0.3 9,932 - -0.5 9,932 - -1.8 9,932 - -0.1 10,086 + EX31_RS16635 0.10 +1.1 10,180 - EX31_RS16635 0.17 +0.4 10,234 - EX31_RS16635 0.21 +1.0 10,234 - EX31_RS16635 0.21 +0.2 10,234 - EX31_RS16635 0.21 -1.3 10,342 - EX31_RS16635 0.29 +0.2 10,484 + EX31_RS16635 0.40 -0.2 10,622 + EX31_RS16635 0.50 -1.1 10,623 - EX31_RS16635 0.50 -0.5 10,685 + EX31_RS16635 0.55 +1.1
Or see this region's nucleotide sequence