Strain Fitness in Rahnella sp. WP5 around EX31_RS16630

Experiment: casaminos (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX31_RS16620 and EX31_RS16625 are separated by 192 nucleotidesEX31_RS16625 and EX31_RS16630 are separated by 27 nucleotidesEX31_RS16630 and EX31_RS16635 are separated by 16 nucleotides EX31_RS16620: EX31_RS16620 - glycosyltransferase, at 6,098 to 7,258 _RS16620 EX31_RS16625: EX31_RS16625 - bifunctional 4-hydroxy-2-oxoglutarate aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase, at 7,451 to 8,089 _RS16625 EX31_RS16630: EX31_RS16630 - phosphogluconate dehydratase, at 8,117 to 9,931 _RS16630 EX31_RS16635: EX31_RS16635 - gluconate transporter, at 9,948 to 11,285 _RS16635 Position (kb) 8 9 10Strain fitness (log2 ratio) -2 -1 0 1 2 3at 7.217 kb on + strandat 7.281 kb on + strandat 7.328 kb on - strandat 7.328 kb on - strandat 7.328 kb on - strandat 7.328 kb on - strandat 7.337 kb on - strandat 7.337 kb on - strandat 7.408 kb on - strandat 7.429 kb on + strandat 7.432 kb on + strandat 7.452 kb on + strandat 7.489 kb on + strandat 7.490 kb on - strandat 7.493 kb on - strandat 7.493 kb on - strandat 7.576 kb on - strand, within EX31_RS16625at 8.126 kb on - strandat 8.438 kb on + strand, within EX31_RS16630at 8.438 kb on + strand, within EX31_RS16630at 8.465 kb on - strand, within EX31_RS16630at 8.465 kb on - strand, within EX31_RS16630at 8.831 kb on + strand, within EX31_RS16630at 8.832 kb on - strand, within EX31_RS16630at 8.832 kb on - strand, within EX31_RS16630at 8.832 kb on - strand, within EX31_RS16630at 8.900 kb on - strand, within EX31_RS16630at 8.924 kb on - strand, within EX31_RS16630at 8.924 kb on - strand, within EX31_RS16630at 9.105 kb on + strand, within EX31_RS16630at 9.106 kb on - strand, within EX31_RS16630at 9.106 kb on - strand, within EX31_RS16630at 9.339 kb on + strand, within EX31_RS16630at 9.340 kb on - strand, within EX31_RS16630at 9.447 kb on - strand, within EX31_RS16630at 9.473 kb on - strand, within EX31_RS16630at 9.473 kb on - strand, within EX31_RS16630at 9.481 kb on - strand, within EX31_RS16630at 9.719 kb on - strand, within EX31_RS16630at 9.742 kb on + strand, within EX31_RS16630at 9.932 kb on - strandat 9.932 kb on - strandat 9.932 kb on - strandat 9.932 kb on - strandat 10.086 kb on + strand, within EX31_RS16635at 10.180 kb on - strand, within EX31_RS16635at 10.234 kb on - strand, within EX31_RS16635at 10.234 kb on - strand, within EX31_RS16635at 10.234 kb on - strand, within EX31_RS16635at 10.342 kb on - strand, within EX31_RS16635at 10.484 kb on + strand, within EX31_RS16635at 10.622 kb on + strand, within EX31_RS16635at 10.623 kb on - strand, within EX31_RS16635at 10.685 kb on + strand, within EX31_RS16635

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Per-strain Table

Position Strand Gene LocusTag Fraction casaminos (C)
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7,217 + +1.4
7,281 + +1.1
7,328 - +1.5
7,328 - -0.4
7,328 - +1.8
7,328 - +0.6
7,337 - -0.2
7,337 - +1.1
7,408 - +0.5
7,429 + -0.5
7,432 + -0.9
7,452 + +3.5
7,489 + +2.1
7,490 - +0.8
7,493 - +1.7
7,493 - -0.5
7,576 - EX31_RS16625 0.20 -0.3
8,126 - -1.3
8,438 + EX31_RS16630 0.18 +0.5
8,438 + EX31_RS16630 0.18 -1.2
8,465 - EX31_RS16630 0.19 +0.9
8,465 - EX31_RS16630 0.19 +0.5
8,831 + EX31_RS16630 0.39 +0.5
8,832 - EX31_RS16630 0.39 +0.5
8,832 - EX31_RS16630 0.39 -1.1
8,832 - EX31_RS16630 0.39 +1.1
8,900 - EX31_RS16630 0.43 -0.5
8,924 - EX31_RS16630 0.44 +1.1
8,924 - EX31_RS16630 0.44 -0.1
9,105 + EX31_RS16630 0.54 +2.1
9,106 - EX31_RS16630 0.54 +0.3
9,106 - EX31_RS16630 0.54 +0.2
9,339 + EX31_RS16630 0.67 +0.9
9,340 - EX31_RS16630 0.67 +0.8
9,447 - EX31_RS16630 0.73 +0.8
9,473 - EX31_RS16630 0.75 -1.4
9,473 - EX31_RS16630 0.75 +0.8
9,481 - EX31_RS16630 0.75 -0.5
9,719 - EX31_RS16630 0.88 +0.9
9,742 + EX31_RS16630 0.90 +2.1
9,932 - -0.3
9,932 - -0.5
9,932 - -1.8
9,932 - -0.1
10,086 + EX31_RS16635 0.10 +1.1
10,180 - EX31_RS16635 0.17 +0.4
10,234 - EX31_RS16635 0.21 +1.0
10,234 - EX31_RS16635 0.21 +0.2
10,234 - EX31_RS16635 0.21 -1.3
10,342 - EX31_RS16635 0.29 +0.2
10,484 + EX31_RS16635 0.40 -0.2
10,622 + EX31_RS16635 0.50 -1.1
10,623 - EX31_RS16635 0.50 -0.5
10,685 + EX31_RS16635 0.55 +1.1

Or see this region's nucleotide sequence