Experiment: casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS14315 and EX31_RS14320 are separated by 152 nucleotides EX31_RS14320 and EX31_RS14325 overlap by 4 nucleotides EX31_RS14325 and EX31_RS14330 are separated by 37 nucleotides EX31_RS14330 and EX31_RS14335 are separated by 52 nucleotides
EX31_RS14315: EX31_RS14315 - periplasmic heavy metal sensor, at 45,224 to 45,733
_RS14315
EX31_RS14320: EX31_RS14320 - envelope stress response regulator transcription factor CpxR, at 45,886 to 46,587
_RS14320
EX31_RS14325: EX31_RS14325 - envelope stress sensor histidine kinase CpxA, at 46,584 to 47,954
_RS14325
EX31_RS14330: EX31_RS14330 - tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL, at 47,992 to 48,465
_RS14330
EX31_RS14335: EX31_RS14335 - serine O-acetyltransferase, at 48,518 to 49,339
_RS14335
Position (kb)
46
47
48 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 45.625 kb on + strand, within EX31_RS14315 at 45.625 kb on + strand, within EX31_RS14315 at 45.626 kb on - strand, within EX31_RS14315 at 45.626 kb on - strand, within EX31_RS14315 at 45.626 kb on - strand, within EX31_RS14315 at 45.626 kb on - strand, within EX31_RS14315 at 45.626 kb on - strand, within EX31_RS14315 at 45.727 kb on + strand at 45.727 kb on + strand at 45.738 kb on + strand at 45.738 kb on + strand at 45.738 kb on + strand at 45.738 kb on + strand at 45.738 kb on + strand at 45.738 kb on + strand at 45.738 kb on + strand at 45.738 kb on + strand at 45.738 kb on + strand at 45.738 kb on + strand at 45.739 kb on - strand at 45.739 kb on - strand at 45.739 kb on - strand at 45.739 kb on - strand at 45.739 kb on - strand at 45.739 kb on - strand at 45.739 kb on - strand at 45.739 kb on - strand at 45.745 kb on - strand at 45.754 kb on - strand at 45.786 kb on + strand at 45.820 kb on + strand at 45.820 kb on + strand at 45.820 kb on + strand at 45.820 kb on + strand at 45.821 kb on - strand at 45.830 kb on + strand at 45.830 kb on + strand at 45.830 kb on + strand at 45.830 kb on + strand at 45.831 kb on - strand at 45.831 kb on - strand at 45.831 kb on - strand at 45.861 kb on + strand at 45.861 kb on + strand at 45.861 kb on + strand at 45.862 kb on - strand at 45.872 kb on + strand at 45.878 kb on + strand at 45.878 kb on + strand at 45.882 kb on + strand at 45.882 kb on + strand at 45.882 kb on + strand at 45.883 kb on - strand at 45.902 kb on + strand at 45.902 kb on + strand at 45.998 kb on + strand, within EX31_RS14320 at 46.011 kb on + strand, within EX31_RS14320 at 46.110 kb on + strand, within EX31_RS14320 at 46.110 kb on + strand, within EX31_RS14320 at 46.136 kb on + strand, within EX31_RS14320 at 46.192 kb on - strand, within EX31_RS14320 at 46.304 kb on + strand, within EX31_RS14320 at 46.476 kb on + strand, within EX31_RS14320 at 46.588 kb on + strand at 46.589 kb on - strand at 46.599 kb on - strand at 46.607 kb on + strand at 46.607 kb on + strand at 46.607 kb on + strand at 46.607 kb on + strand at 46.608 kb on - strand at 46.640 kb on - strand at 46.640 kb on - strand at 46.654 kb on + strand at 46.654 kb on + strand at 46.655 kb on - strand at 46.655 kb on - strand at 46.655 kb on - strand at 46.658 kb on - strand at 46.688 kb on + strand at 46.897 kb on + strand, within EX31_RS14325 at 46.897 kb on + strand, within EX31_RS14325 at 46.897 kb on + strand, within EX31_RS14325 at 46.897 kb on + strand, within EX31_RS14325 at 46.898 kb on - strand, within EX31_RS14325 at 46.898 kb on - strand, within EX31_RS14325 at 46.898 kb on - strand, within EX31_RS14325 at 46.898 kb on - strand, within EX31_RS14325 at 46.898 kb on - strand, within EX31_RS14325 at 46.898 kb on - strand, within EX31_RS14325 at 46.901 kb on + strand, within EX31_RS14325 at 46.901 kb on + strand, within EX31_RS14325 at 46.901 kb on + strand, within EX31_RS14325 at 47.004 kb on + strand, within EX31_RS14325 at 47.005 kb on - strand, within EX31_RS14325 at 47.005 kb on - strand, within EX31_RS14325 at 47.157 kb on - strand, within EX31_RS14325 at 47.157 kb on - strand, within EX31_RS14325 at 47.157 kb on - strand, within EX31_RS14325 at 47.157 kb on - strand, within EX31_RS14325 at 47.290 kb on + strand, within EX31_RS14325 at 47.290 kb on + strand, within EX31_RS14325 at 47.291 kb on - strand, within EX31_RS14325 at 47.291 kb on - strand, within EX31_RS14325 at 47.291 kb on - strand, within EX31_RS14325 at 47.332 kb on + strand, within EX31_RS14325 at 47.332 kb on + strand, within EX31_RS14325 at 47.332 kb on + strand, within EX31_RS14325 at 47.332 kb on + strand, within EX31_RS14325 at 47.332 kb on + strand, within EX31_RS14325 at 47.332 kb on + strand, within EX31_RS14325 at 47.332 kb on + strand, within EX31_RS14325 at 47.332 kb on + strand, within EX31_RS14325 at 47.332 kb on + strand, within EX31_RS14325 at 47.332 kb on + strand, within EX31_RS14325 at 47.332 kb on + strand, within EX31_RS14325 at 47.333 kb on - strand, within EX31_RS14325 at 47.333 kb on - strand, within EX31_RS14325 at 47.333 kb on - strand, within EX31_RS14325 at 47.333 kb on - strand, within EX31_RS14325 at 47.333 kb on - strand, within EX31_RS14325 at 47.333 kb on - strand, within EX31_RS14325 at 47.333 kb on - strand, within EX31_RS14325 at 47.333 kb on - strand, within EX31_RS14325 at 47.333 kb on - strand, within EX31_RS14325 at 47.333 kb on - strand, within EX31_RS14325 at 47.333 kb on - strand, within EX31_RS14325 at 47.337 kb on - strand, within EX31_RS14325 at 47.337 kb on - strand, within EX31_RS14325 at 47.464 kb on + strand, within EX31_RS14325 at 47.481 kb on - strand, within EX31_RS14325 at 47.481 kb on - strand, within EX31_RS14325 at 47.481 kb on - strand, within EX31_RS14325 at 47.492 kb on - strand, within EX31_RS14325 at 47.492 kb on - strand, within EX31_RS14325 at 47.727 kb on - strand, within EX31_RS14325 at 47.887 kb on + strand at 47.888 kb on - strand at 47.888 kb on - strand at 47.963 kb on + strand at 47.964 kb on - strand at 47.978 kb on + strand at 47.979 kb on - strand at 47.997 kb on + strand at 47.997 kb on + strand at 47.997 kb on + strand at 47.997 kb on + strand at 47.997 kb on + strand at 47.998 kb on - strand at 47.998 kb on - strand at 47.998 kb on - strand at 47.998 kb on - strand at 48.008 kb on + strand at 48.008 kb on + strand at 48.009 kb on - strand at 48.143 kb on - strand, within EX31_RS14330 at 48.143 kb on - strand, within EX31_RS14330 at 48.159 kb on + strand, within EX31_RS14330 at 48.160 kb on - strand, within EX31_RS14330 at 48.161 kb on + strand, within EX31_RS14330 at 48.161 kb on + strand, within EX31_RS14330 at 48.161 kb on + strand, within EX31_RS14330 at 48.161 kb on + strand, within EX31_RS14330 at 48.161 kb on + strand, within EX31_RS14330 at 48.161 kb on + strand, within EX31_RS14330 at 48.161 kb on + strand, within EX31_RS14330 at 48.161 kb on + strand, within EX31_RS14330 at 48.161 kb on + strand, within EX31_RS14330 at 48.161 kb on + strand, within EX31_RS14330 at 48.161 kb on + strand, within EX31_RS14330 at 48.161 kb on + strand, within EX31_RS14330 at 48.161 kb on + strand, within EX31_RS14330 at 48.161 kb on + strand, within EX31_RS14330 at 48.161 kb on + strand, within EX31_RS14330 at 48.161 kb on + strand, within EX31_RS14330 at 48.161 kb on + strand, within EX31_RS14330 at 48.161 kb on + strand, within EX31_RS14330 at 48.161 kb on + strand, within EX31_RS14330 at 48.162 kb on - strand, within EX31_RS14330 at 48.162 kb on - strand, within EX31_RS14330 at 48.162 kb on - strand, within EX31_RS14330 at 48.162 kb on - strand, within EX31_RS14330 at 48.162 kb on - strand, within EX31_RS14330 at 48.162 kb on - strand, within EX31_RS14330 at 48.162 kb on - strand, within EX31_RS14330 at 48.162 kb on - strand, within EX31_RS14330 at 48.162 kb on - strand, within EX31_RS14330 at 48.162 kb on - strand, within EX31_RS14330 at 48.162 kb on - strand, within EX31_RS14330 at 48.162 kb on - strand, within EX31_RS14330 at 48.162 kb on - strand, within EX31_RS14330 at 48.162 kb on - strand, within EX31_RS14330 at 48.178 kb on + strand, within EX31_RS14330 at 48.178 kb on + strand, within EX31_RS14330 at 48.178 kb on + strand, within EX31_RS14330 at 48.178 kb on + strand, within EX31_RS14330 at 48.179 kb on - strand, within EX31_RS14330 at 48.179 kb on - strand, within EX31_RS14330 at 48.179 kb on - strand, within EX31_RS14330 at 48.262 kb on + strand, within EX31_RS14330 at 48.262 kb on + strand, within EX31_RS14330 at 48.263 kb on - strand, within EX31_RS14330 at 48.263 kb on - strand, within EX31_RS14330 at 48.263 kb on - strand, within EX31_RS14330 at 48.318 kb on + strand, within EX31_RS14330 at 48.319 kb on - strand, within EX31_RS14330 at 48.319 kb on - strand, within EX31_RS14330 at 48.319 kb on - strand, within EX31_RS14330 at 48.319 kb on - strand, within EX31_RS14330 at 48.319 kb on - strand, within EX31_RS14330 at 48.360 kb on + strand, within EX31_RS14330 at 48.361 kb on - strand, within EX31_RS14330 at 48.415 kb on + strand, within EX31_RS14330 at 48.415 kb on + strand, within EX31_RS14330 at 48.416 kb on - strand, within EX31_RS14330 at 48.416 kb on - strand, within EX31_RS14330 at 48.464 kb on - strand at 48.553 kb on + strand at 48.553 kb on + strand at 48.554 kb on - strand at 48.574 kb on + strand at 48.575 kb on - strand at 48.707 kb on - strand, within EX31_RS14335 at 48.707 kb on - strand, within EX31_RS14335 at 48.757 kb on + strand, within EX31_RS14335 at 48.788 kb on - strand, within EX31_RS14335 at 48.819 kb on - strand, within EX31_RS14335 at 48.910 kb on + strand, within EX31_RS14335 at 48.911 kb on - strand, within EX31_RS14335 at 48.914 kb on + strand, within EX31_RS14335
Per-strain Table
Position Strand Gene LocusTag Fraction casaminos (C) remove 45,625 + EX31_RS14315 0.79 -0.8 45,625 + EX31_RS14315 0.79 -0.8 45,626 - EX31_RS14315 0.79 +0.0 45,626 - EX31_RS14315 0.79 +0.7 45,626 - EX31_RS14315 0.79 -0.2 45,626 - EX31_RS14315 0.79 +0.6 45,626 - EX31_RS14315 0.79 +0.9 45,727 + +1.6 45,727 + -0.1 45,738 + -0.6 45,738 + -2.2 45,738 + +0.1 45,738 + +0.6 45,738 + -0.0 45,738 + +0.6 45,738 + -0.4 45,738 + -0.3 45,738 + +0.6 45,738 + -1.7 45,739 - +0.8 45,739 - +0.1 45,739 - -0.6 45,739 - +0.4 45,739 - -1.2 45,739 - -0.3 45,739 - -0.1 45,739 - -0.1 45,745 - -0.3 45,754 - -0.9 45,786 + -0.2 45,820 + -0.7 45,820 + +0.5 45,820 + -1.3 45,820 + -2.1 45,821 - -1.1 45,830 + -0.4 45,830 + +2.0 45,830 + +0.3 45,830 + +0.0 45,831 - +0.6 45,831 - -0.1 45,831 - +0.7 45,861 + +0.6 45,861 + -0.6 45,861 + -0.1 45,862 - +1.6 45,872 + -0.4 45,878 + +0.9 45,878 + +0.9 45,882 + -0.8 45,882 + -1.4 45,882 + -1.0 45,883 - +1.8 45,902 + +0.6 45,902 + +0.8 45,998 + EX31_RS14320 0.16 -0.0 46,011 + EX31_RS14320 0.18 +1.6 46,110 + EX31_RS14320 0.32 +1.6 46,110 + EX31_RS14320 0.32 -0.4 46,136 + EX31_RS14320 0.36 +0.8 46,192 - EX31_RS14320 0.44 +2.2 46,304 + EX31_RS14320 0.60 +0.2 46,476 + EX31_RS14320 0.84 -0.8 46,588 + +0.4 46,589 - -1.6 46,599 - -2.8 46,607 + -0.2 46,607 + -1.1 46,607 + -1.6 46,607 + +0.3 46,608 - -0.6 46,640 - -2.5 46,640 - -0.1 46,654 + -0.9 46,654 + -1.0 46,655 - -0.4 46,655 - -0.6 46,655 - -1.3 46,658 - -1.4 46,688 + -0.8 46,897 + EX31_RS14325 0.23 -1.2 46,897 + EX31_RS14325 0.23 -0.7 46,897 + EX31_RS14325 0.23 +0.4 46,897 + EX31_RS14325 0.23 -1.2 46,898 - EX31_RS14325 0.23 -2.7 46,898 - EX31_RS14325 0.23 +0.6 46,898 - EX31_RS14325 0.23 -2.5 46,898 - EX31_RS14325 0.23 -1.0 46,898 - EX31_RS14325 0.23 -0.4 46,898 - EX31_RS14325 0.23 -0.0 46,901 + EX31_RS14325 0.23 -1.1 46,901 + EX31_RS14325 0.23 -0.4 46,901 + EX31_RS14325 0.23 +0.2 47,004 + EX31_RS14325 0.31 -3.0 47,005 - EX31_RS14325 0.31 -1.0 47,005 - EX31_RS14325 0.31 -0.2 47,157 - EX31_RS14325 0.42 -1.0 47,157 - EX31_RS14325 0.42 -0.3 47,157 - EX31_RS14325 0.42 +0.2 47,157 - EX31_RS14325 0.42 -1.1 47,290 + EX31_RS14325 0.51 -0.7 47,290 + EX31_RS14325 0.51 -2.0 47,291 - EX31_RS14325 0.52 -3.0 47,291 - EX31_RS14325 0.52 +0.8 47,291 - EX31_RS14325 0.52 -0.7 47,332 + EX31_RS14325 0.55 -1.8 47,332 + EX31_RS14325 0.55 -1.9 47,332 + EX31_RS14325 0.55 +0.2 47,332 + EX31_RS14325 0.55 -2.2 47,332 + EX31_RS14325 0.55 -3.5 47,332 + EX31_RS14325 0.55 +0.7 47,332 + EX31_RS14325 0.55 -0.4 47,332 + EX31_RS14325 0.55 -2.0 47,332 + EX31_RS14325 0.55 -2.5 47,332 + EX31_RS14325 0.55 -1.0 47,332 + EX31_RS14325 0.55 -2.3 47,333 - EX31_RS14325 0.55 -2.6 47,333 - EX31_RS14325 0.55 -1.8 47,333 - EX31_RS14325 0.55 -0.0 47,333 - EX31_RS14325 0.55 -0.9 47,333 - EX31_RS14325 0.55 -1.8 47,333 - EX31_RS14325 0.55 -2.1 47,333 - EX31_RS14325 0.55 -1.3 47,333 - EX31_RS14325 0.55 +1.6 47,333 - EX31_RS14325 0.55 -1.9 47,333 - EX31_RS14325 0.55 -0.7 47,333 - EX31_RS14325 0.55 -1.2 47,337 - EX31_RS14325 0.55 -0.9 47,337 - EX31_RS14325 0.55 -0.4 47,464 + EX31_RS14325 0.64 +1.6 47,481 - EX31_RS14325 0.65 -0.5 47,481 - EX31_RS14325 0.65 -1.1 47,481 - EX31_RS14325 0.65 -0.6 47,492 - EX31_RS14325 0.66 -1.0 47,492 - EX31_RS14325 0.66 +0.4 47,727 - EX31_RS14325 0.83 -0.3 47,887 + -1.0 47,888 - -0.4 47,888 - -2.5 47,963 + -1.4 47,964 - -1.4 47,978 + +0.9 47,979 - -1.0 47,997 + -0.0 47,997 + -0.6 47,997 + -1.6 47,997 + +0.3 47,997 + +0.7 47,998 - +0.5 47,998 - +1.8 47,998 - +0.4 47,998 - +0.3 48,008 + +0.2 48,008 + +0.7 48,009 - +0.5 48,143 - EX31_RS14330 0.32 +1.0 48,143 - EX31_RS14330 0.32 -0.5 48,159 + EX31_RS14330 0.35 +2.0 48,160 - EX31_RS14330 0.35 +0.9 48,161 + EX31_RS14330 0.36 -0.8 48,161 + EX31_RS14330 0.36 -0.7 48,161 + EX31_RS14330 0.36 -0.5 48,161 + EX31_RS14330 0.36 +0.2 48,161 + EX31_RS14330 0.36 +0.8 48,161 + EX31_RS14330 0.36 -0.0 48,161 + EX31_RS14330 0.36 -1.0 48,161 + EX31_RS14330 0.36 -1.8 48,161 + EX31_RS14330 0.36 +1.0 48,161 + EX31_RS14330 0.36 -0.1 48,161 + EX31_RS14330 0.36 +0.2 48,161 + EX31_RS14330 0.36 +0.8 48,161 + EX31_RS14330 0.36 -0.0 48,161 + EX31_RS14330 0.36 -0.2 48,161 + EX31_RS14330 0.36 -0.8 48,161 + EX31_RS14330 0.36 +0.6 48,161 + EX31_RS14330 0.36 -1.8 48,161 + EX31_RS14330 0.36 +1.0 48,161 + EX31_RS14330 0.36 +0.4 48,162 - EX31_RS14330 0.36 +0.0 48,162 - EX31_RS14330 0.36 +1.0 48,162 - EX31_RS14330 0.36 -0.4 48,162 - EX31_RS14330 0.36 +0.3 48,162 - EX31_RS14330 0.36 +1.3 48,162 - EX31_RS14330 0.36 +0.2 48,162 - EX31_RS14330 0.36 +0.8 48,162 - EX31_RS14330 0.36 +0.8 48,162 - EX31_RS14330 0.36 -0.3 48,162 - EX31_RS14330 0.36 -0.7 48,162 - EX31_RS14330 0.36 +1.8 48,162 - EX31_RS14330 0.36 +0.6 48,162 - EX31_RS14330 0.36 +0.7 48,162 - EX31_RS14330 0.36 +0.6 48,178 + EX31_RS14330 0.39 -0.9 48,178 + EX31_RS14330 0.39 +0.4 48,178 + EX31_RS14330 0.39 -0.1 48,178 + EX31_RS14330 0.39 +0.1 48,179 - EX31_RS14330 0.39 +0.6 48,179 - EX31_RS14330 0.39 -0.6 48,179 - EX31_RS14330 0.39 +0.1 48,262 + EX31_RS14330 0.57 +0.4 48,262 + EX31_RS14330 0.57 -0.8 48,263 - EX31_RS14330 0.57 -0.1 48,263 - EX31_RS14330 0.57 -0.4 48,263 - EX31_RS14330 0.57 -0.4 48,318 + EX31_RS14330 0.69 -0.0 48,319 - EX31_RS14330 0.69 +0.9 48,319 - EX31_RS14330 0.69 +0.6 48,319 - EX31_RS14330 0.69 +0.3 48,319 - EX31_RS14330 0.69 +0.1 48,319 - EX31_RS14330 0.69 +0.3 48,360 + EX31_RS14330 0.78 -1.0 48,361 - EX31_RS14330 0.78 -0.8 48,415 + EX31_RS14330 0.89 +0.3 48,415 + EX31_RS14330 0.89 -0.6 48,416 - EX31_RS14330 0.89 +0.3 48,416 - EX31_RS14330 0.89 -0.2 48,464 - +0.4 48,553 + -0.6 48,553 + -2.1 48,554 - +0.1 48,574 + +1.7 48,575 - +0.1 48,707 - EX31_RS14335 0.23 -1.8 48,707 - EX31_RS14335 0.23 +0.6 48,757 + EX31_RS14335 0.29 -1.2 48,788 - EX31_RS14335 0.33 -0.4 48,819 - EX31_RS14335 0.37 -0.8 48,910 + EX31_RS14335 0.48 +0.6 48,911 - EX31_RS14335 0.48 -1.9 48,914 + EX31_RS14335 0.48 -1.4
Or see this region's nucleotide sequence