Strain Fitness in Rahnella sp. WP5 around EX31_RS10180

Experiment: casaminos (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX31_RS10170 and EX31_RS10175 overlap by 4 nucleotidesEX31_RS10175 and EX31_RS10180 are separated by 16 nucleotidesEX31_RS10180 and EX31_RS10185 are separated by 71 nucleotides EX31_RS10170: EX31_RS10170 - gallate dioxygenase, at 246,659 to 247,921 _RS10170 EX31_RS10175: EX31_RS10175 - amidohydrolase family protein, at 247,918 to 248,751 _RS10175 EX31_RS10180: EX31_RS10180 - type II 3-dehydroquinate dehydratase, at 248,768 to 249,208 _RS10180 EX31_RS10185: EX31_RS10185 - O-antigen ligase family protein, at 249,280 to 250,533 _RS10185 Position (kb) 248 249 250Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 247.821 kb on + strandat 247.821 kb on + strandat 247.821 kb on + strandat 247.822 kb on - strandat 247.822 kb on - strandat 247.825 kb on + strandat 247.825 kb on + strandat 247.825 kb on + strandat 247.825 kb on + strandat 247.825 kb on + strandat 247.825 kb on + strandat 247.825 kb on + strandat 247.825 kb on + strandat 247.826 kb on - strandat 247.826 kb on - strandat 247.826 kb on - strandat 247.826 kb on - strandat 247.829 kb on + strandat 247.830 kb on - strandat 247.830 kb on - strandat 248.002 kb on + strand, within EX31_RS10175at 248.041 kb on + strand, within EX31_RS10175at 248.063 kb on - strand, within EX31_RS10175at 248.063 kb on - strand, within EX31_RS10175at 248.105 kb on - strand, within EX31_RS10175at 248.106 kb on + strand, within EX31_RS10175at 248.106 kb on + strand, within EX31_RS10175at 248.106 kb on + strand, within EX31_RS10175at 248.106 kb on + strand, within EX31_RS10175at 248.106 kb on + strand, within EX31_RS10175at 248.107 kb on - strand, within EX31_RS10175at 248.107 kb on - strand, within EX31_RS10175at 248.107 kb on - strand, within EX31_RS10175at 248.554 kb on + strand, within EX31_RS10175at 248.775 kb on - strandat 248.775 kb on - strandat 248.838 kb on - strand, within EX31_RS10180at 249.138 kb on + strand, within EX31_RS10180at 249.139 kb on - strand, within EX31_RS10180at 249.171 kb on - strandat 249.582 kb on + strand, within EX31_RS10185at 249.621 kb on + strand, within EX31_RS10185at 249.630 kb on + strand, within EX31_RS10185at 249.648 kb on - strand, within EX31_RS10185at 249.650 kb on - strand, within EX31_RS10185at 249.651 kb on + strand, within EX31_RS10185at 249.652 kb on - strand, within EX31_RS10185at 249.652 kb on - strand, within EX31_RS10185at 249.707 kb on + strand, within EX31_RS10185at 249.838 kb on - strand, within EX31_RS10185at 249.844 kb on - strand, within EX31_RS10185at 250.004 kb on + strand, within EX31_RS10185at 250.119 kb on + strand, within EX31_RS10185at 250.124 kb on - strand, within EX31_RS10185

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Per-strain Table

Position Strand Gene LocusTag Fraction casaminos (C)
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247,821 + +1.4
247,821 + -1.4
247,821 + +2.4
247,822 - +0.3
247,822 - +0.2
247,825 + -0.6
247,825 + -0.2
247,825 + -1.7
247,825 + +0.6
247,825 + +0.8
247,825 + +2.2
247,825 + +3.0
247,825 + +0.4
247,826 - -0.2
247,826 - +0.6
247,826 - -0.1
247,826 - +1.5
247,829 + -0.3
247,830 - +2.6
247,830 - -0.7
248,002 + EX31_RS10175 0.10 -0.2
248,041 + EX31_RS10175 0.15 -0.0
248,063 - EX31_RS10175 0.17 +1.7
248,063 - EX31_RS10175 0.17 -0.2
248,105 - EX31_RS10175 0.22 -3.1
248,106 + EX31_RS10175 0.23 +0.1
248,106 + EX31_RS10175 0.23 -0.6
248,106 + EX31_RS10175 0.23 +0.9
248,106 + EX31_RS10175 0.23 -0.8
248,106 + EX31_RS10175 0.23 -0.4
248,107 - EX31_RS10175 0.23 -0.4
248,107 - EX31_RS10175 0.23 -1.0
248,107 - EX31_RS10175 0.23 +0.9
248,554 + EX31_RS10175 0.76 -0.0
248,775 - +0.6
248,775 - +0.6
248,838 - EX31_RS10180 0.16 -1.7
249,138 + EX31_RS10180 0.84 +0.6
249,139 - EX31_RS10180 0.84 -0.4
249,171 - -0.7
249,582 + EX31_RS10185 0.24 +0.3
249,621 + EX31_RS10185 0.27 +0.6
249,630 + EX31_RS10185 0.28 +0.6
249,648 - EX31_RS10185 0.29 +2.6
249,650 - EX31_RS10185 0.30 -1.0
249,651 + EX31_RS10185 0.30 +0.6
249,652 - EX31_RS10185 0.30 -0.4
249,652 - EX31_RS10185 0.30 +0.2
249,707 + EX31_RS10185 0.34 +1.1
249,838 - EX31_RS10185 0.44 -1.0
249,844 - EX31_RS10185 0.45 +0.3
250,004 + EX31_RS10185 0.58 -2.0
250,119 + EX31_RS10185 0.67 -0.0
250,124 - EX31_RS10185 0.67 +1.3

Or see this region's nucleotide sequence