Experiment: casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS09475 and EX31_RS25295 are separated by 118 nucleotides EX31_RS25295 and EX31_RS25300 are separated by 133 nucleotides EX31_RS25300 and EX31_RS09490 are separated by 70 nucleotides EX31_RS09490 and EX31_RS09495 are separated by 41 nucleotides EX31_RS09495 and EX31_RS09500 are separated by 233 nucleotides
EX31_RS09475: EX31_RS09475 - AraC family transcriptional regulator, at 104,438 to 105,292
_RS09475
EX31_RS25295: EX31_RS25295 - GhoT/OrtT family toxin, at 105,411 to 105,587
_RS25295
EX31_RS25300: EX31_RS25300 - YqaE/Pmp3 family membrane protein, at 105,721 to 105,879
_RS25300
EX31_RS09490: EX31_RS09490 - hypothetical protein, at 105,950 to 106,204
_RS09490
EX31_RS09495: EX31_RS09495 - DUF4186 domain-containing protein, at 106,246 to 106,767
_RS09495
EX31_RS09500: EX31_RS09500 - hypothetical protein, at 107,001 to 107,276
_RS09500
Position (kb)
105
106
107 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 104.954 kb on - strand, within EX31_RS09475 at 104.954 kb on - strand, within EX31_RS09475 at 104.958 kb on + strand, within EX31_RS09475 at 104.958 kb on + strand, within EX31_RS09475 at 104.958 kb on + strand, within EX31_RS09475 at 104.959 kb on - strand, within EX31_RS09475 at 104.968 kb on - strand, within EX31_RS09475 at 104.986 kb on + strand, within EX31_RS09475 at 104.995 kb on + strand, within EX31_RS09475 at 105.096 kb on + strand, within EX31_RS09475 at 105.096 kb on + strand, within EX31_RS09475 at 105.097 kb on - strand, within EX31_RS09475 at 105.151 kb on + strand, within EX31_RS09475 at 105.151 kb on + strand, within EX31_RS09475 at 105.151 kb on + strand, within EX31_RS09475 at 105.166 kb on - strand, within EX31_RS09475 at 105.166 kb on - strand, within EX31_RS09475 at 105.171 kb on - strand, within EX31_RS09475 at 105.171 kb on - strand, within EX31_RS09475 at 105.171 kb on - strand, within EX31_RS09475 at 105.259 kb on + strand at 105.288 kb on + strand at 105.288 kb on + strand at 105.288 kb on + strand at 105.288 kb on + strand at 105.289 kb on - strand at 105.289 kb on - strand at 105.289 kb on - strand at 105.289 kb on - strand at 105.289 kb on - strand at 105.289 kb on - strand at 105.289 kb on - strand at 105.290 kb on + strand at 105.290 kb on + strand at 105.317 kb on - strand at 105.344 kb on - strand at 105.344 kb on - strand at 105.374 kb on + strand at 105.374 kb on + strand at 105.374 kb on + strand at 105.374 kb on + strand at 105.374 kb on + strand at 105.374 kb on + strand at 105.389 kb on + strand at 105.389 kb on + strand at 105.434 kb on + strand, within EX31_RS25295 at 105.444 kb on + strand, within EX31_RS25295 at 105.508 kb on + strand, within EX31_RS25295 at 105.612 kb on + strand at 105.612 kb on + strand at 105.612 kb on + strand at 105.612 kb on + strand at 105.612 kb on + strand at 105.612 kb on + strand at 105.612 kb on + strand at 105.665 kb on + strand at 105.830 kb on - strand, within EX31_RS25300 at 105.863 kb on - strand, within EX31_RS25300 at 105.883 kb on - strand at 105.951 kb on + strand at 105.951 kb on + strand at 105.952 kb on - strand at 105.971 kb on - strand at 106.094 kb on - strand, within EX31_RS09490 at 106.094 kb on - strand, within EX31_RS09490 at 106.115 kb on - strand, within EX31_RS09490 at 106.184 kb on + strand at 106.184 kb on + strand at 106.184 kb on + strand at 106.184 kb on + strand at 106.185 kb on - strand at 106.185 kb on - strand at 106.228 kb on + strand at 106.242 kb on + strand at 106.243 kb on - strand at 106.330 kb on - strand, within EX31_RS09495 at 106.471 kb on - strand, within EX31_RS09495 at 106.608 kb on + strand, within EX31_RS09495 at 106.608 kb on + strand, within EX31_RS09495 at 106.699 kb on - strand, within EX31_RS09495 at 106.765 kb on + strand at 106.766 kb on - strand at 106.766 kb on - strand at 106.869 kb on - strand at 106.954 kb on + strand at 106.971 kb on + strand at 106.972 kb on - strand at 107.016 kb on + strand at 107.016 kb on + strand at 107.016 kb on + strand at 107.135 kb on + strand, within EX31_RS09500 at 107.136 kb on - strand, within EX31_RS09500 at 107.153 kb on + strand, within EX31_RS09500 at 107.196 kb on + strand, within EX31_RS09500 at 107.196 kb on + strand, within EX31_RS09500 at 107.196 kb on + strand, within EX31_RS09500 at 107.197 kb on - strand, within EX31_RS09500
Per-strain Table
Position Strand Gene LocusTag Fraction casaminos (C) remove 104,954 - EX31_RS09475 0.60 -2.2 104,954 - EX31_RS09475 0.60 +0.6 104,958 + EX31_RS09475 0.61 +1.0 104,958 + EX31_RS09475 0.61 -0.4 104,958 + EX31_RS09475 0.61 +0.3 104,959 - EX31_RS09475 0.61 +0.5 104,968 - EX31_RS09475 0.62 +1.2 104,986 + EX31_RS09475 0.64 -0.0 104,995 + EX31_RS09475 0.65 +2.9 105,096 + EX31_RS09475 0.77 +0.6 105,096 + EX31_RS09475 0.77 -0.4 105,097 - EX31_RS09475 0.77 -0.0 105,151 + EX31_RS09475 0.83 -1.4 105,151 + EX31_RS09475 0.83 +1.0 105,151 + EX31_RS09475 0.83 -0.4 105,166 - EX31_RS09475 0.85 -1.0 105,166 - EX31_RS09475 0.85 -0.4 105,171 - EX31_RS09475 0.86 +0.9 105,171 - EX31_RS09475 0.86 +0.6 105,171 - EX31_RS09475 0.86 -0.6 105,259 + -1.6 105,288 + +0.6 105,288 + -0.1 105,288 + -0.2 105,288 + +1.1 105,289 - +0.9 105,289 - -0.1 105,289 - +0.6 105,289 - -1.0 105,289 - +1.6 105,289 - +0.7 105,289 - +1.2 105,290 + -0.8 105,290 + +1.6 105,317 - +0.2 105,344 - +1.8 105,344 - -0.8 105,374 + -0.6 105,374 + +1.6 105,374 + +1.0 105,374 + -0.6 105,374 + +0.1 105,374 + +1.6 105,389 + +0.8 105,389 + -0.5 105,434 + EX31_RS25295 0.13 +2.0 105,444 + EX31_RS25295 0.19 -0.9 105,508 + EX31_RS25295 0.55 +1.6 105,612 + -1.9 105,612 + -0.2 105,612 + +1.6 105,612 + -0.5 105,612 + +1.2 105,612 + +2.0 105,612 + +1.1 105,665 + +1.2 105,830 - EX31_RS25300 0.69 -0.6 105,863 - EX31_RS25300 0.89 +2.8 105,883 - -1.0 105,951 + +0.6 105,951 + +0.9 105,952 - -0.0 105,971 - +1.3 106,094 - EX31_RS09490 0.56 -1.0 106,094 - EX31_RS09490 0.56 -0.9 106,115 - EX31_RS09490 0.65 -0.4 106,184 + +1.6 106,184 + +0.6 106,184 + +1.6 106,184 + -0.4 106,185 - +1.2 106,185 - -0.1 106,228 + -0.4 106,242 + +1.3 106,243 - -0.8 106,330 - EX31_RS09495 0.16 +0.4 106,471 - EX31_RS09495 0.43 +0.8 106,608 + EX31_RS09495 0.69 -1.0 106,608 + EX31_RS09495 0.69 +0.2 106,699 - EX31_RS09495 0.87 -0.2 106,765 + +0.6 106,766 - +1.9 106,766 - +1.0 106,869 - -0.3 106,954 + +0.9 106,971 + +0.8 106,972 - -0.0 107,016 + +0.4 107,016 + +1.0 107,016 + +0.2 107,135 + EX31_RS09500 0.49 +1.5 107,136 - EX31_RS09500 0.49 -1.3 107,153 + EX31_RS09500 0.55 +2.0 107,196 + EX31_RS09500 0.71 -0.4 107,196 + EX31_RS09500 0.71 -0.1 107,196 + EX31_RS09500 0.71 -2.1 107,197 - EX31_RS09500 0.71 -1.0
Or see this region's nucleotide sequence