Strain Fitness in Rahnella sp. WP5 around EX31_RS09335

Experiment: casaminos (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX31_RS09330 and EX31_RS09335 are separated by 413 nucleotidesEX31_RS09335 and EX31_RS09340 are separated by 53 nucleotidesEX31_RS09340 and EX31_RS09345 are separated by 114 nucleotides EX31_RS09330: EX31_RS09330 - chloride channel protein, at 69,225 to 70,928 _RS09330 EX31_RS09335: EX31_RS09335 - hypothetical protein, at 71,342 to 71,656 _RS09335 EX31_RS09340: EX31_RS09340 - HigA family addiction module antidote protein, at 71,710 to 72,027 _RS09340 EX31_RS09345: EX31_RS09345 - ABC transporter substrate-binding protein, at 72,142 to 73,719 _RS09345 Position (kb) 71 72Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 70.409 kb on - strand, within EX31_RS09330at 70.409 kb on - strand, within EX31_RS09330at 70.409 kb on - strand, within EX31_RS09330at 70.448 kb on + strand, within EX31_RS09330at 70.448 kb on + strand, within EX31_RS09330at 70.448 kb on + strand, within EX31_RS09330at 70.449 kb on - strand, within EX31_RS09330at 70.449 kb on - strand, within EX31_RS09330at 70.462 kb on - strand, within EX31_RS09330at 70.929 kb on - strandat 71.124 kb on + strandat 71.124 kb on + strandat 71.124 kb on + strandat 71.125 kb on - strandat 71.125 kb on - strandat 71.250 kb on - strandat 71.250 kb on - strandat 71.318 kb on + strandat 71.424 kb on + strand, within EX31_RS09335at 71.496 kb on + strand, within EX31_RS09335at 71.496 kb on + strand, within EX31_RS09335at 71.519 kb on + strand, within EX31_RS09335at 71.621 kb on + strand, within EX31_RS09335at 71.625 kb on + strandat 71.625 kb on + strandat 71.625 kb on + strandat 71.625 kb on + strandat 71.625 kb on + strandat 71.654 kb on + strandat 71.659 kb on - strandat 71.666 kb on + strandat 71.692 kb on + strandat 71.692 kb on + strandat 71.692 kb on + strandat 71.709 kb on + strandat 71.709 kb on + strandat 71.727 kb on + strandat 71.727 kb on + strandat 71.753 kb on + strand, within EX31_RS09340at 71.753 kb on + strand, within EX31_RS09340at 71.826 kb on + strand, within EX31_RS09340at 71.826 kb on + strand, within EX31_RS09340at 71.847 kb on + strand, within EX31_RS09340at 71.943 kb on + strand, within EX31_RS09340at 71.985 kb on + strand, within EX31_RS09340at 72.013 kb on - strandat 72.143 kb on + strandat 72.144 kb on - strandat 72.293 kb on + strandat 72.413 kb on + strand, within EX31_RS09345at 72.413 kb on + strand, within EX31_RS09345at 72.414 kb on - strand, within EX31_RS09345at 72.414 kb on - strand, within EX31_RS09345at 72.423 kb on + strand, within EX31_RS09345at 72.424 kb on - strand, within EX31_RS09345at 72.617 kb on - strand, within EX31_RS09345at 72.627 kb on + strand, within EX31_RS09345at 72.627 kb on + strand, within EX31_RS09345

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Per-strain Table

Position Strand Gene LocusTag Fraction casaminos (C)
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70,409 - EX31_RS09330 0.69 -2.0
70,409 - EX31_RS09330 0.69 +0.9
70,409 - EX31_RS09330 0.69 -1.0
70,448 + EX31_RS09330 0.72 +1.1
70,448 + EX31_RS09330 0.72 +2.6
70,448 + EX31_RS09330 0.72 +1.0
70,449 - EX31_RS09330 0.72 -0.4
70,449 - EX31_RS09330 0.72 -0.4
70,462 - EX31_RS09330 0.73 -2.6
70,929 - +0.2
71,124 + +1.6
71,124 + -0.4
71,124 + +1.6
71,125 - +0.9
71,125 - +0.4
71,250 - +0.4
71,250 - -0.2
71,318 + -0.4
71,424 + EX31_RS09335 0.26 +1.6
71,496 + EX31_RS09335 0.49 -1.6
71,496 + EX31_RS09335 0.49 -1.4
71,519 + EX31_RS09335 0.56 -0.4
71,621 + EX31_RS09335 0.89 +1.2
71,625 + -1.0
71,625 + -1.2
71,625 + -0.4
71,625 + +0.6
71,625 + -0.6
71,654 + +2.2
71,659 - +1.6
71,666 + -0.9
71,692 + -0.4
71,692 + +0.4
71,692 + +1.8
71,709 + -2.1
71,709 + -0.6
71,727 + -2.2
71,727 + -2.7
71,753 + EX31_RS09340 0.14 -0.1
71,753 + EX31_RS09340 0.14 -0.6
71,826 + EX31_RS09340 0.36 -2.0
71,826 + EX31_RS09340 0.36 -2.4
71,847 + EX31_RS09340 0.43 +3.8
71,943 + EX31_RS09340 0.73 +0.4
71,985 + EX31_RS09340 0.86 -0.7
72,013 - +0.2
72,143 + -0.4
72,144 - +0.8
72,293 + +0.6
72,413 + EX31_RS09345 0.17 -0.7
72,413 + EX31_RS09345 0.17 +0.6
72,414 - EX31_RS09345 0.17 -0.4
72,414 - EX31_RS09345 0.17 -0.1
72,423 + EX31_RS09345 0.18 -0.2
72,424 - EX31_RS09345 0.18 -1.2
72,617 - EX31_RS09345 0.30 +1.4
72,627 + EX31_RS09345 0.31 +1.6
72,627 + EX31_RS09345 0.31 -0.4

Or see this region's nucleotide sequence