Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS35845 and BPHYT_RS35850 are separated by 8 nucleotides BPHYT_RS35850 and BPHYT_RS35855 are separated by 6 nucleotides BPHYT_RS35855 and BPHYT_RS35860 are separated by 23 nucleotides
BPHYT_RS35845: BPHYT_RS35845 - hypothetical protein, at 2,404 to 3,792
_RS35845
BPHYT_RS35850: BPHYT_RS35850 - hypothetical protein, at 3,801 to 4,601
_RS35850
BPHYT_RS35855: BPHYT_RS35855 - type II secretion system protein E, at 4,608 to 6,626
_RS35855
BPHYT_RS35860: BPHYT_RS35860 - type II secretory pathway component PulF-like protein, at 6,650 to 7,768
_RS35860
Position (kb) 4
5
6
7 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 3.799 kb on + strand at 3.995 kb on + strand, within BPHYT_RS35850 at 4.077 kb on - strand at 4.308 kb on + strand, within BPHYT_RS35850 at 4.308 kb on + strand, within BPHYT_RS35850 at 4.318 kb on - strand, within BPHYT_RS35850 at 4.519 kb on + strand, within BPHYT_RS35850 at 4.519 kb on + strand, within BPHYT_RS35850 at 4.520 kb on - strand, within BPHYT_RS35850 at 4.599 kb on + strand at 4.599 kb on + strand at 4.599 kb on + strand at 4.690 kb on + strand at 5.222 kb on + strand, within BPHYT_RS35855 at 5.223 kb on - strand, within BPHYT_RS35855 at 5.261 kb on - strand, within BPHYT_RS35855 at 5.293 kb on - strand, within BPHYT_RS35855 at 5.375 kb on + strand, within BPHYT_RS35855 at 5.376 kb on - strand, within BPHYT_RS35855 at 5.378 kb on - strand, within BPHYT_RS35855 at 5.390 kb on + strand, within BPHYT_RS35855 at 5.390 kb on + strand, within BPHYT_RS35855 at 5.391 kb on - strand, within BPHYT_RS35855 at 5.614 kb on - strand, within BPHYT_RS35855 at 5.624 kb on + strand, within BPHYT_RS35855 at 5.920 kb on + strand, within BPHYT_RS35855 at 5.952 kb on - strand, within BPHYT_RS35855 at 5.952 kb on - strand, within BPHYT_RS35855 at 6.298 kb on - strand, within BPHYT_RS35855 at 6.386 kb on + strand, within BPHYT_RS35855 at 6.489 kb on + strand at 6.580 kb on + strand at 6.581 kb on - strand at 6.649 kb on + strand at 6.649 kb on + strand at 6.650 kb on - strand at 6.650 kb on - strand at 6.675 kb on + strand at 6.675 kb on + strand at 6.676 kb on - strand at 6.741 kb on - strand at 6.741 kb on - strand at 6.931 kb on + strand, within BPHYT_RS35860 at 6.932 kb on - strand, within BPHYT_RS35860 at 7.149 kb on + strand, within BPHYT_RS35860 at 7.149 kb on + strand, within BPHYT_RS35860 at 7.149 kb on + strand, within BPHYT_RS35860 at 7.150 kb on - strand, within BPHYT_RS35860 at 7.150 kb on - strand, within BPHYT_RS35860 at 7.185 kb on - strand, within BPHYT_RS35860 at 7.185 kb on - strand, within BPHYT_RS35860 at 7.185 kb on - strand, within BPHYT_RS35860 at 7.265 kb on + strand, within BPHYT_RS35860
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 3,799 + -1.9 3,995 + BPHYT_RS35850 0.24 +0.2 4,077 - -0.6 4,308 + BPHYT_RS35850 0.63 -0.1 4,308 + BPHYT_RS35850 0.63 -2.9 4,318 - BPHYT_RS35850 0.65 -0.3 4,519 + BPHYT_RS35850 0.90 +0.1 4,519 + BPHYT_RS35850 0.90 -1.2 4,520 - BPHYT_RS35850 0.90 -0.3 4,599 + -1.7 4,599 + -0.6 4,599 + -0.5 4,690 + +0.4 5,222 + BPHYT_RS35855 0.30 -1.0 5,223 - BPHYT_RS35855 0.30 +0.5 5,261 - BPHYT_RS35855 0.32 -2.9 5,293 - BPHYT_RS35855 0.34 +0.6 5,375 + BPHYT_RS35855 0.38 +0.6 5,376 - BPHYT_RS35855 0.38 +0.1 5,378 - BPHYT_RS35855 0.38 +0.8 5,390 + BPHYT_RS35855 0.39 +0.5 5,390 + BPHYT_RS35855 0.39 +0.7 5,391 - BPHYT_RS35855 0.39 +1.2 5,614 - BPHYT_RS35855 0.50 +0.8 5,624 + BPHYT_RS35855 0.50 -0.2 5,920 + BPHYT_RS35855 0.65 -0.4 5,952 - BPHYT_RS35855 0.67 +0.3 5,952 - BPHYT_RS35855 0.67 +1.3 6,298 - BPHYT_RS35855 0.84 +0.3 6,386 + BPHYT_RS35855 0.88 +1.2 6,489 + -1.0 6,580 + -1.9 6,581 - +1.0 6,649 + +0.3 6,649 + -0.4 6,650 - -1.1 6,650 - -0.0 6,675 + -2.1 6,675 + -0.1 6,676 - +1.0 6,741 - -0.8 6,741 - -0.1 6,931 + BPHYT_RS35860 0.25 -0.6 6,932 - BPHYT_RS35860 0.25 -0.1 7,149 + BPHYT_RS35860 0.45 -1.2 7,149 + BPHYT_RS35860 0.45 +0.9 7,149 + BPHYT_RS35860 0.45 -0.6 7,150 - BPHYT_RS35860 0.45 +0.7 7,150 - BPHYT_RS35860 0.45 -0.3 7,185 - BPHYT_RS35860 0.48 -0.9 7,185 - BPHYT_RS35860 0.48 +0.1 7,185 - BPHYT_RS35860 0.48 +0.9 7,265 + BPHYT_RS35860 0.55 -1.2
Or see this region's nucleotide sequence