Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS32720 and BPHYT_RS32725 are separated by 11 nucleotides BPHYT_RS32725 and BPHYT_RS32730 are separated by 242 nucleotides BPHYT_RS32730 and BPHYT_RS32735 are separated by 18 nucleotides BPHYT_RS32735 and BPHYT_RS32740 are separated by 109 nucleotides
BPHYT_RS32720: BPHYT_RS32720 - membrane protein, at 2,920,980 to 2,921,720
_RS32720
BPHYT_RS32725: BPHYT_RS32725 - cobalt transporter, at 2,921,732 to 2,921,953
_RS32725
BPHYT_RS32730: BPHYT_RS32730 - histidine phosphatase, at 2,922,196 to 2,922,792
_RS32730
BPHYT_RS32735: BPHYT_RS32735 - hypothetical protein, at 2,922,811 to 2,923,251
_RS32735
BPHYT_RS32740: BPHYT_RS32740 - hypothetical protein, at 2,923,361 to 2,924,377
_RS32740
Position (kb)
2922
2923 Strain fitness (log2 ratio)
-1
0
1
2 at 2921.711 kb on + strand at 2921.711 kb on + strand at 2921.712 kb on - strand at 2921.787 kb on + strand, within BPHYT_RS32725 at 2921.787 kb on + strand, within BPHYT_RS32725 at 2921.788 kb on - strand, within BPHYT_RS32725 at 2921.788 kb on - strand, within BPHYT_RS32725 at 2921.788 kb on - strand, within BPHYT_RS32725 at 2922.044 kb on - strand at 2922.044 kb on - strand at 2922.103 kb on + strand at 2922.180 kb on - strand at 2922.180 kb on - strand at 2922.612 kb on + strand, within BPHYT_RS32730 at 2922.705 kb on - strand, within BPHYT_RS32730 at 2922.867 kb on + strand, within BPHYT_RS32735 at 2922.896 kb on + strand, within BPHYT_RS32735 at 2922.897 kb on - strand, within BPHYT_RS32735 at 2923.028 kb on + strand, within BPHYT_RS32735 at 2923.285 kb on + strand at 2923.285 kb on + strand at 2923.492 kb on + strand, within BPHYT_RS32740 at 2923.494 kb on + strand, within BPHYT_RS32740 at 2923.669 kb on - strand, within BPHYT_RS32740 at 2923.692 kb on + strand, within BPHYT_RS32740 at 2923.791 kb on + strand, within BPHYT_RS32740 at 2923.791 kb on + strand, within BPHYT_RS32740 at 2923.792 kb on - strand, within BPHYT_RS32740 at 2923.792 kb on - strand, within BPHYT_RS32740
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 2,921,711 + +0.6 2,921,711 + +0.0 2,921,712 - -1.1 2,921,787 + BPHYT_RS32725 0.25 +0.2 2,921,787 + BPHYT_RS32725 0.25 -0.8 2,921,788 - BPHYT_RS32725 0.25 -1.4 2,921,788 - BPHYT_RS32725 0.25 -0.4 2,921,788 - BPHYT_RS32725 0.25 -0.3 2,922,044 - -0.2 2,922,044 - +0.2 2,922,103 + +1.2 2,922,180 - +0.2 2,922,180 - +0.2 2,922,612 + BPHYT_RS32730 0.70 -1.0 2,922,705 - BPHYT_RS32730 0.85 -0.2 2,922,867 + BPHYT_RS32735 0.13 +2.1 2,922,896 + BPHYT_RS32735 0.19 +1.2 2,922,897 - BPHYT_RS32735 0.20 -0.8 2,923,028 + BPHYT_RS32735 0.49 +0.8 2,923,285 + +0.1 2,923,285 + -0.4 2,923,492 + BPHYT_RS32740 0.13 -0.6 2,923,494 + BPHYT_RS32740 0.13 -1.4 2,923,669 - BPHYT_RS32740 0.30 +0.4 2,923,692 + BPHYT_RS32740 0.33 +0.2 2,923,791 + BPHYT_RS32740 0.42 +0.8 2,923,791 + BPHYT_RS32740 0.42 -0.3 2,923,792 - BPHYT_RS32740 0.42 +0.5 2,923,792 - BPHYT_RS32740 0.42 -0.6
Or see this region's nucleotide sequence