Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS32730

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS32720 and BPHYT_RS32725 are separated by 11 nucleotidesBPHYT_RS32725 and BPHYT_RS32730 are separated by 242 nucleotidesBPHYT_RS32730 and BPHYT_RS32735 are separated by 18 nucleotidesBPHYT_RS32735 and BPHYT_RS32740 are separated by 109 nucleotides BPHYT_RS32720: BPHYT_RS32720 - membrane protein, at 2,920,980 to 2,921,720 _RS32720 BPHYT_RS32725: BPHYT_RS32725 - cobalt transporter, at 2,921,732 to 2,921,953 _RS32725 BPHYT_RS32730: BPHYT_RS32730 - histidine phosphatase, at 2,922,196 to 2,922,792 _RS32730 BPHYT_RS32735: BPHYT_RS32735 - hypothetical protein, at 2,922,811 to 2,923,251 _RS32735 BPHYT_RS32740: BPHYT_RS32740 - hypothetical protein, at 2,923,361 to 2,924,377 _RS32740 Position (kb) 2922 2923Strain fitness (log2 ratio) -1 0 1 2at 2921.711 kb on + strandat 2921.711 kb on + strandat 2921.712 kb on - strandat 2921.787 kb on + strand, within BPHYT_RS32725at 2921.787 kb on + strand, within BPHYT_RS32725at 2921.788 kb on - strand, within BPHYT_RS32725at 2921.788 kb on - strand, within BPHYT_RS32725at 2921.788 kb on - strand, within BPHYT_RS32725at 2922.044 kb on - strandat 2922.044 kb on - strandat 2922.103 kb on + strandat 2922.180 kb on - strandat 2922.180 kb on - strandat 2922.612 kb on + strand, within BPHYT_RS32730at 2922.705 kb on - strand, within BPHYT_RS32730at 2922.867 kb on + strand, within BPHYT_RS32735at 2922.896 kb on + strand, within BPHYT_RS32735at 2922.897 kb on - strand, within BPHYT_RS32735at 2923.028 kb on + strand, within BPHYT_RS32735at 2923.285 kb on + strandat 2923.285 kb on + strandat 2923.492 kb on + strand, within BPHYT_RS32740at 2923.494 kb on + strand, within BPHYT_RS32740at 2923.669 kb on - strand, within BPHYT_RS32740at 2923.692 kb on + strand, within BPHYT_RS32740at 2923.791 kb on + strand, within BPHYT_RS32740at 2923.791 kb on + strand, within BPHYT_RS32740at 2923.792 kb on - strand, within BPHYT_RS32740at 2923.792 kb on - strand, within BPHYT_RS32740

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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2,921,711 + +0.6
2,921,711 + +0.0
2,921,712 - -1.1
2,921,787 + BPHYT_RS32725 0.25 +0.2
2,921,787 + BPHYT_RS32725 0.25 -0.8
2,921,788 - BPHYT_RS32725 0.25 -1.4
2,921,788 - BPHYT_RS32725 0.25 -0.4
2,921,788 - BPHYT_RS32725 0.25 -0.3
2,922,044 - -0.2
2,922,044 - +0.2
2,922,103 + +1.2
2,922,180 - +0.2
2,922,180 - +0.2
2,922,612 + BPHYT_RS32730 0.70 -1.0
2,922,705 - BPHYT_RS32730 0.85 -0.2
2,922,867 + BPHYT_RS32735 0.13 +2.1
2,922,896 + BPHYT_RS32735 0.19 +1.2
2,922,897 - BPHYT_RS32735 0.20 -0.8
2,923,028 + BPHYT_RS32735 0.49 +0.8
2,923,285 + +0.1
2,923,285 + -0.4
2,923,492 + BPHYT_RS32740 0.13 -0.6
2,923,494 + BPHYT_RS32740 0.13 -1.4
2,923,669 - BPHYT_RS32740 0.30 +0.4
2,923,692 + BPHYT_RS32740 0.33 +0.2
2,923,791 + BPHYT_RS32740 0.42 +0.8
2,923,791 + BPHYT_RS32740 0.42 -0.3
2,923,792 - BPHYT_RS32740 0.42 +0.5
2,923,792 - BPHYT_RS32740 0.42 -0.6

Or see this region's nucleotide sequence