Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS30135

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS30130 and BPHYT_RS30135 are separated by 230 nucleotidesBPHYT_RS30135 and BPHYT_RS30140 are separated by 256 nucleotides BPHYT_RS30130: BPHYT_RS30130 - AraC family transcriptional regulator, at 2,311,464 to 2,312,450 _RS30130 BPHYT_RS30135: BPHYT_RS30135 - SAM-dependent methyltransferase, at 2,312,681 to 2,313,514 _RS30135 BPHYT_RS30140: BPHYT_RS30140 - 3-methylitaconate isomerase, at 2,313,771 to 2,314,958 _RS30140 Position (kb) 2312 2313 2314Strain fitness (log2 ratio) -2 -1 0 1 2at 2311.845 kb on - strand, within BPHYT_RS30130at 2312.093 kb on - strand, within BPHYT_RS30130at 2312.765 kb on + strand, within BPHYT_RS30135at 2313.109 kb on - strand, within BPHYT_RS30135at 2313.356 kb on + strand, within BPHYT_RS30135at 2313.597 kb on + strandat 2313.597 kb on + strandat 2313.606 kb on - strandat 2314.002 kb on - strand, within BPHYT_RS30140at 2314.002 kb on - strand, within BPHYT_RS30140

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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2,311,845 - BPHYT_RS30130 0.39 -1.9
2,312,093 - BPHYT_RS30130 0.64 -0.6
2,312,765 + BPHYT_RS30135 0.10 +0.3
2,313,109 - BPHYT_RS30135 0.51 +1.9
2,313,356 + BPHYT_RS30135 0.81 -0.1
2,313,597 + -0.1
2,313,597 + +0.2
2,313,606 - -0.0
2,314,002 - BPHYT_RS30140 0.19 -0.8
2,314,002 - BPHYT_RS30140 0.19 -0.2

Or see this region's nucleotide sequence