Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS28740

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS28730 and BPHYT_RS28735 are separated by 160 nucleotidesBPHYT_RS28735 and BPHYT_RS28740 are separated by 29 nucleotidesBPHYT_RS28740 and BPHYT_RS28745 are separated by 13 nucleotidesBPHYT_RS28745 and BPHYT_RS28750 are separated by 65 nucleotides BPHYT_RS28730: BPHYT_RS28730 - galactarate dehydratase, at 1,999,039 to 2,000,550 _RS28730 BPHYT_RS28735: BPHYT_RS28735 - hypothetical protein, at 2,000,711 to 2,001,406 _RS28735 BPHYT_RS28740: BPHYT_RS28740 - 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase, at 2,001,436 to 2,002,326 _RS28740 BPHYT_RS28745: BPHYT_RS28745 - 2-keto-4-pentenoate hydratase, at 2,002,340 to 2,003,149 _RS28745 BPHYT_RS28750: BPHYT_RS28750 - IclR family transcriptional regulator, at 2,003,215 to 2,003,499 _RS28750 Position (kb) 2001 2002 2003Strain fitness (log2 ratio) -1 0 1at 2000.817 kb on - strand, within BPHYT_RS28735at 2000.878 kb on + strand, within BPHYT_RS28735at 2000.879 kb on - strand, within BPHYT_RS28735at 2001.073 kb on - strand, within BPHYT_RS28735at 2001.077 kb on - strand, within BPHYT_RS28735at 2001.077 kb on - strand, within BPHYT_RS28735at 2001.406 kb on + strandat 2001.438 kb on - strandat 2001.480 kb on + strandat 2001.489 kb on - strandat 2001.609 kb on + strand, within BPHYT_RS28740at 2001.651 kb on - strand, within BPHYT_RS28740at 2001.810 kb on + strand, within BPHYT_RS28740at 2002.428 kb on + strand, within BPHYT_RS28745at 2002.778 kb on - strand, within BPHYT_RS28745at 2002.782 kb on - strand, within BPHYT_RS28745at 2002.843 kb on + strand, within BPHYT_RS28745

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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2,000,817 - BPHYT_RS28735 0.15 -1.5
2,000,878 + BPHYT_RS28735 0.24 +0.3
2,000,879 - BPHYT_RS28735 0.24 -0.1
2,001,073 - BPHYT_RS28735 0.52 +0.3
2,001,077 - BPHYT_RS28735 0.53 -0.3
2,001,077 - BPHYT_RS28735 0.53 -0.1
2,001,406 + +0.0
2,001,438 - +0.4
2,001,480 + -1.2
2,001,489 - +1.0
2,001,609 + BPHYT_RS28740 0.19 +0.7
2,001,651 - BPHYT_RS28740 0.24 +0.3
2,001,810 + BPHYT_RS28740 0.42 +0.8
2,002,428 + BPHYT_RS28745 0.11 +1.7
2,002,778 - BPHYT_RS28745 0.54 +1.4
2,002,782 - BPHYT_RS28745 0.55 -1.3
2,002,843 + BPHYT_RS28745 0.62 +1.6

Or see this region's nucleotide sequence