Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS28320

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS28315 and BPHYT_RS28320 are separated by 4 nucleotidesBPHYT_RS28320 and BPHYT_RS28325 are separated by 459 nucleotides BPHYT_RS28315: BPHYT_RS28315 - transporter, at 1,906,546 to 1,907,223 _RS28315 BPHYT_RS28320: BPHYT_RS28320 - molecular chaperone DnaJ, at 1,907,228 to 1,907,905 _RS28320 BPHYT_RS28325: BPHYT_RS28325 - NADPH-dependent FMN reductase, at 1,908,365 to 1,909,024 _RS28325 Position (kb) 1907 1908Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1906.250 kb on - strandat 1906.286 kb on + strandat 1906.299 kb on + strandat 1906.299 kb on + strandat 1906.305 kb on + strandat 1906.305 kb on + strandat 1906.305 kb on + strandat 1906.306 kb on - strandat 1906.429 kb on + strandat 1906.472 kb on - strandat 1906.488 kb on + strandat 1906.565 kb on + strandat 1906.580 kb on - strandat 1907.158 kb on + strandat 1907.159 kb on - strandat 1907.161 kb on - strandat 1907.161 kb on - strandat 1907.701 kb on - strand, within BPHYT_RS28320at 1907.892 kb on + strandat 1907.892 kb on + strandat 1907.892 kb on + strandat 1907.947 kb on + strandat 1907.968 kb on - strandat 1908.087 kb on - strandat 1908.126 kb on - strandat 1908.229 kb on + strandat 1908.533 kb on + strand, within BPHYT_RS28325at 1908.701 kb on + strand, within BPHYT_RS28325

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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1,906,250 - +0.3
1,906,286 + +1.3
1,906,299 + -0.2
1,906,299 + +0.3
1,906,305 + +0.1
1,906,305 + +1.2
1,906,305 + +0.2
1,906,306 - +0.4
1,906,429 + +2.3
1,906,472 - -2.8
1,906,488 + +0.4
1,906,565 + -0.6
1,906,580 - +1.3
1,907,158 + -0.6
1,907,159 - -0.1
1,907,161 - -0.7
1,907,161 - -0.2
1,907,701 - BPHYT_RS28320 0.70 +0.9
1,907,892 + -1.3
1,907,892 + +0.3
1,907,892 + +0.3
1,907,947 + +0.3
1,907,968 - -0.3
1,908,087 - +0.3
1,908,126 - -0.0
1,908,229 + -0.3
1,908,533 + BPHYT_RS28325 0.25 -1.2
1,908,701 + BPHYT_RS28325 0.51 -1.9

Or see this region's nucleotide sequence