Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS26540

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS26535 and BPHYT_RS26540 are separated by 120 nucleotidesBPHYT_RS26540 and BPHYT_RS26545 are separated by 61 nucleotides BPHYT_RS26535: BPHYT_RS26535 - GntR family transcriptional regulator, at 1,521,326 to 1,522,735 _RS26535 BPHYT_RS26540: BPHYT_RS26540 - hypothetical protein, at 1,522,856 to 1,523,941 _RS26540 BPHYT_RS26545: BPHYT_RS26545 - hypothetical protein, at 1,524,003 to 1,524,962 _RS26545 Position (kb) 1522 1523 1524Strain fitness (log2 ratio) -1 0 1at 1521.929 kb on + strand, within BPHYT_RS26535at 1522.164 kb on + strand, within BPHYT_RS26535at 1522.165 kb on - strand, within BPHYT_RS26535at 1523.343 kb on - strand, within BPHYT_RS26540at 1523.913 kb on + strandat 1523.913 kb on + strandat 1524.054 kb on + strandat 1524.368 kb on - strand, within BPHYT_RS26545

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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1,521,929 + BPHYT_RS26535 0.43 +0.2
1,522,164 + BPHYT_RS26535 0.59 -0.7
1,522,165 - BPHYT_RS26535 0.60 -0.4
1,523,343 - BPHYT_RS26540 0.45 +0.9
1,523,913 + -0.5
1,523,913 + -1.6
1,524,054 + -0.0
1,524,368 - BPHYT_RS26545 0.38 -0.3

Or see this region's nucleotide sequence