Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS25350

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS25345 and BPHYT_RS25350 are separated by 374 nucleotidesBPHYT_RS25350 and BPHYT_RS25355 are separated by 91 nucleotides BPHYT_RS25345: BPHYT_RS25345 - arylsulfatase, at 1,260,505 to 1,262,157 _RS25345 BPHYT_RS25350: BPHYT_RS25350 - AMP-dependent synthetase and ligase, at 1,262,532 to 1,264,232 _RS25350 BPHYT_RS25355: BPHYT_RS25355 - LuxR family transcriptional regulator, at 1,264,324 to 1,267,026 _RS25355 Position (kb) 1262 1263 1264 1265Strain fitness (log2 ratio) -1 0 1 2at 1262.149 kb on - strandat 1262.261 kb on + strandat 1262.517 kb on + strandat 1262.517 kb on + strandat 1262.519 kb on + strandat 1262.520 kb on - strandat 1263.066 kb on - strand, within BPHYT_RS25350at 1263.266 kb on + strand, within BPHYT_RS25350at 1263.266 kb on + strand, within BPHYT_RS25350at 1263.314 kb on - strand, within BPHYT_RS25350at 1263.613 kb on - strand, within BPHYT_RS25350at 1264.156 kb on - strandat 1264.201 kb on + strandat 1264.610 kb on + strand, within BPHYT_RS25355at 1264.610 kb on + strand, within BPHYT_RS25355at 1264.611 kb on - strand, within BPHYT_RS25355at 1265.185 kb on + strand, within BPHYT_RS25355

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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1,262,149 - -0.4
1,262,261 + -0.9
1,262,517 + +2.2
1,262,517 + +0.1
1,262,519 + -1.1
1,262,520 - -1.0
1,263,066 - BPHYT_RS25350 0.31 +0.8
1,263,266 + BPHYT_RS25350 0.43 +0.4
1,263,266 + BPHYT_RS25350 0.43 +0.8
1,263,314 - BPHYT_RS25350 0.46 +0.6
1,263,613 - BPHYT_RS25350 0.64 +1.4
1,264,156 - -0.9
1,264,201 + +0.2
1,264,610 + BPHYT_RS25355 0.11 +0.8
1,264,610 + BPHYT_RS25355 0.11 +0.3
1,264,611 - BPHYT_RS25355 0.11 +0.3
1,265,185 + BPHYT_RS25355 0.32 +2.0

Or see this region's nucleotide sequence