Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS19885 and BPHYT_RS19890 are separated by 368 nucleotides BPHYT_RS19890 and BPHYT_RS19895 are separated by 47 nucleotides BPHYT_RS19895 and BPHYT_RS19900 are separated by 303 nucleotides BPHYT_RS19900 and BPHYT_RS19905 are separated by 279 nucleotides
BPHYT_RS19885: BPHYT_RS19885 - hypothetical protein, at 3,938 to 5,911
_RS19885
BPHYT_RS19890: BPHYT_RS19890 - hypothetical protein, at 6,280 to 6,783
_RS19890
BPHYT_RS19895: BPHYT_RS19895 - rod shape-determining protein RodA, at 6,831 to 7,265
_RS19895
BPHYT_RS19900: BPHYT_RS19900 - hypothetical protein, at 7,569 to 7,850
_RS19900
BPHYT_RS19905: BPHYT_RS19905 - membrane protein, at 8,130 to 9,530
_RS19905
Position (kb) 6
7
8 Strain fitness (log2 ratio) -2
-1
0
1 at 5.943 kb on - strand at 6.035 kb on - strand at 6.038 kb on - strand at 6.112 kb on + strand at 6.113 kb on - strand at 6.128 kb on + strand at 6.129 kb on - strand at 6.129 kb on - strand at 6.129 kb on - strand at 6.379 kb on - strand, within BPHYT_RS19890 at 6.939 kb on - strand, within BPHYT_RS19895 at 7.653 kb on + strand, within BPHYT_RS19900
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 5,943 - -0.1 6,035 - -0.2 6,038 - -2.0 6,112 + -0.2 6,113 - -0.4 6,128 + -1.0 6,129 - +1.0 6,129 - +0.2 6,129 - -1.0 6,379 - BPHYT_RS19890 0.20 +0.8 6,939 - BPHYT_RS19895 0.25 +0.8 7,653 + BPHYT_RS19900 0.30 -0.5
Or see this region's nucleotide sequence