Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS19010 and BPHYT_RS19015 are separated by 21 nucleotides BPHYT_RS19015 and BPHYT_RS19020 are separated by 221 nucleotides BPHYT_RS19020 and BPHYT_RS19025 are separated by 51 nucleotides
BPHYT_RS19010: BPHYT_RS19010 - flagellar motor protein MotB, at 4,268,782 to 4,269,789
_RS19010
BPHYT_RS19015: BPHYT_RS19015 - flagellar motor protein MotA, at 4,269,811 to 4,270,671
_RS19015
BPHYT_RS19020: BPHYT_RS19020 - transcriptional regulator, at 4,270,893 to 4,271,453
_RS19020
BPHYT_RS19025: BPHYT_RS19025 - transcriptional regulator, at 4,271,505 to 4,271,825
_RS19025
Position (kb)
4269
4270
4271 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 4269.107 kb on + strand, within BPHYT_RS19010 at 4269.108 kb on - strand, within BPHYT_RS19010 at 4269.128 kb on + strand, within BPHYT_RS19010 at 4269.218 kb on + strand, within BPHYT_RS19010 at 4269.218 kb on + strand, within BPHYT_RS19010 at 4269.218 kb on + strand, within BPHYT_RS19010 at 4269.218 kb on + strand, within BPHYT_RS19010 at 4269.608 kb on + strand, within BPHYT_RS19010 at 4269.608 kb on + strand, within BPHYT_RS19010 at 4269.702 kb on - strand at 4269.812 kb on + strand at 4270.022 kb on + strand, within BPHYT_RS19015 at 4270.286 kb on + strand, within BPHYT_RS19015 at 4270.286 kb on + strand, within BPHYT_RS19015 at 4270.287 kb on - strand, within BPHYT_RS19015 at 4270.287 kb on - strand, within BPHYT_RS19015 at 4270.334 kb on + strand, within BPHYT_RS19015 at 4270.424 kb on + strand, within BPHYT_RS19015 at 4270.425 kb on - strand, within BPHYT_RS19015 at 4270.425 kb on - strand, within BPHYT_RS19015 at 4270.425 kb on - strand, within BPHYT_RS19015 at 4270.425 kb on - strand, within BPHYT_RS19015 at 4270.425 kb on - strand, within BPHYT_RS19015 at 4270.446 kb on + strand at 4270.448 kb on + strand, within BPHYT_RS19015 at 4270.448 kb on + strand, within BPHYT_RS19015 at 4270.448 kb on + strand, within BPHYT_RS19015 at 4270.448 kb on + strand, within BPHYT_RS19015 at 4270.448 kb on + strand, within BPHYT_RS19015 at 4270.449 kb on - strand, within BPHYT_RS19015 at 4270.449 kb on - strand, within BPHYT_RS19015 at 4270.449 kb on - strand, within BPHYT_RS19015 at 4270.463 kb on + strand, within BPHYT_RS19015 at 4270.547 kb on - strand, within BPHYT_RS19015 at 4270.771 kb on + strand at 4271.216 kb on - strand, within BPHYT_RS19020 at 4271.224 kb on + strand, within BPHYT_RS19020 at 4271.225 kb on - strand, within BPHYT_RS19020 at 4271.225 kb on - strand, within BPHYT_RS19020 at 4271.321 kb on + strand, within BPHYT_RS19020 at 4271.321 kb on + strand, within BPHYT_RS19020 at 4271.321 kb on + strand, within BPHYT_RS19020 at 4271.321 kb on + strand, within BPHYT_RS19020 at 4271.321 kb on + strand, within BPHYT_RS19020 at 4271.321 kb on + strand, within BPHYT_RS19020 at 4271.322 kb on - strand, within BPHYT_RS19020 at 4271.324 kb on - strand, within BPHYT_RS19020 at 4271.325 kb on + strand, within BPHYT_RS19020 at 4271.325 kb on + strand, within BPHYT_RS19020 at 4271.325 kb on + strand, within BPHYT_RS19020 at 4271.326 kb on - strand, within BPHYT_RS19020 at 4271.444 kb on + strand at 4271.444 kb on + strand at 4271.444 kb on + strand at 4271.445 kb on - strand at 4271.445 kb on - strand at 4271.446 kb on + strand at 4271.446 kb on + strand at 4271.446 kb on + strand at 4271.447 kb on - strand at 4271.484 kb on + strand at 4271.493 kb on + strand at 4271.494 kb on - strand at 4271.601 kb on + strand, within BPHYT_RS19025 at 4271.601 kb on + strand, within BPHYT_RS19025 at 4271.602 kb on - strand, within BPHYT_RS19025
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 4,269,107 + BPHYT_RS19010 0.32 -5.3 4,269,108 - BPHYT_RS19010 0.32 -2.1 4,269,128 + BPHYT_RS19010 0.34 -2.6 4,269,218 + BPHYT_RS19010 0.43 -3.3 4,269,218 + BPHYT_RS19010 0.43 -3.3 4,269,218 + BPHYT_RS19010 0.43 -3.3 4,269,218 + BPHYT_RS19010 0.43 -5.2 4,269,608 + BPHYT_RS19010 0.82 -4.0 4,269,608 + BPHYT_RS19010 0.82 -4.2 4,269,702 - -0.7 4,269,812 + -4.1 4,270,022 + BPHYT_RS19015 0.25 -3.4 4,270,286 + BPHYT_RS19015 0.55 -2.6 4,270,286 + BPHYT_RS19015 0.55 -1.5 4,270,287 - BPHYT_RS19015 0.55 -4.3 4,270,287 - BPHYT_RS19015 0.55 -4.0 4,270,334 + BPHYT_RS19015 0.61 -3.7 4,270,424 + BPHYT_RS19015 0.71 -5.4 4,270,425 - BPHYT_RS19015 0.71 -4.1 4,270,425 - BPHYT_RS19015 0.71 -2.3 4,270,425 - BPHYT_RS19015 0.71 -1.0 4,270,425 - BPHYT_RS19015 0.71 -2.7 4,270,425 - BPHYT_RS19015 0.71 +1.9 4,270,446 + +1.3 4,270,448 + BPHYT_RS19015 0.74 -4.8 4,270,448 + BPHYT_RS19015 0.74 -4.4 4,270,448 + BPHYT_RS19015 0.74 -3.3 4,270,448 + BPHYT_RS19015 0.74 -3.3 4,270,448 + BPHYT_RS19015 0.74 -2.1 4,270,449 - BPHYT_RS19015 0.74 -4.2 4,270,449 - BPHYT_RS19015 0.74 -5.8 4,270,449 - BPHYT_RS19015 0.74 -4.8 4,270,463 + BPHYT_RS19015 0.76 -1.5 4,270,547 - BPHYT_RS19015 0.85 -2.6 4,270,771 + -1.4 4,271,216 - BPHYT_RS19020 0.58 +0.1 4,271,224 + BPHYT_RS19020 0.59 +0.3 4,271,225 - BPHYT_RS19020 0.59 -2.2 4,271,225 - BPHYT_RS19020 0.59 +1.3 4,271,321 + BPHYT_RS19020 0.76 +0.9 4,271,321 + BPHYT_RS19020 0.76 -1.0 4,271,321 + BPHYT_RS19020 0.76 -0.6 4,271,321 + BPHYT_RS19020 0.76 -0.2 4,271,321 + BPHYT_RS19020 0.76 -1.0 4,271,321 + BPHYT_RS19020 0.76 -0.3 4,271,322 - BPHYT_RS19020 0.76 -1.1 4,271,324 - BPHYT_RS19020 0.77 -1.1 4,271,325 + BPHYT_RS19020 0.77 -0.9 4,271,325 + BPHYT_RS19020 0.77 -0.7 4,271,325 + BPHYT_RS19020 0.77 +0.5 4,271,326 - BPHYT_RS19020 0.77 -0.4 4,271,444 + -2.3 4,271,444 + +0.6 4,271,444 + -1.4 4,271,445 - -0.1 4,271,445 - +0.1 4,271,446 + -0.7 4,271,446 + -0.1 4,271,446 + -3.8 4,271,447 - -1.4 4,271,484 + -0.8 4,271,493 + -0.9 4,271,494 - -0.3 4,271,601 + BPHYT_RS19025 0.30 -0.9 4,271,601 + BPHYT_RS19025 0.30 -0.7 4,271,602 - BPHYT_RS19025 0.30 +0.2
Or see this region's nucleotide sequence