Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS19015

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS19010 and BPHYT_RS19015 are separated by 21 nucleotidesBPHYT_RS19015 and BPHYT_RS19020 are separated by 221 nucleotidesBPHYT_RS19020 and BPHYT_RS19025 are separated by 51 nucleotides BPHYT_RS19010: BPHYT_RS19010 - flagellar motor protein MotB, at 4,268,782 to 4,269,789 _RS19010 BPHYT_RS19015: BPHYT_RS19015 - flagellar motor protein MotA, at 4,269,811 to 4,270,671 _RS19015 BPHYT_RS19020: BPHYT_RS19020 - transcriptional regulator, at 4,270,893 to 4,271,453 _RS19020 BPHYT_RS19025: BPHYT_RS19025 - transcriptional regulator, at 4,271,505 to 4,271,825 _RS19025 Position (kb) 4269 4270 4271Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1 2at 4269.107 kb on + strand, within BPHYT_RS19010at 4269.108 kb on - strand, within BPHYT_RS19010at 4269.128 kb on + strand, within BPHYT_RS19010at 4269.218 kb on + strand, within BPHYT_RS19010at 4269.218 kb on + strand, within BPHYT_RS19010at 4269.218 kb on + strand, within BPHYT_RS19010at 4269.218 kb on + strand, within BPHYT_RS19010at 4269.608 kb on + strand, within BPHYT_RS19010at 4269.608 kb on + strand, within BPHYT_RS19010at 4269.702 kb on - strandat 4269.812 kb on + strandat 4270.022 kb on + strand, within BPHYT_RS19015at 4270.286 kb on + strand, within BPHYT_RS19015at 4270.286 kb on + strand, within BPHYT_RS19015at 4270.287 kb on - strand, within BPHYT_RS19015at 4270.287 kb on - strand, within BPHYT_RS19015at 4270.334 kb on + strand, within BPHYT_RS19015at 4270.424 kb on + strand, within BPHYT_RS19015at 4270.425 kb on - strand, within BPHYT_RS19015at 4270.425 kb on - strand, within BPHYT_RS19015at 4270.425 kb on - strand, within BPHYT_RS19015at 4270.425 kb on - strand, within BPHYT_RS19015at 4270.425 kb on - strand, within BPHYT_RS19015at 4270.446 kb on + strandat 4270.448 kb on + strand, within BPHYT_RS19015at 4270.448 kb on + strand, within BPHYT_RS19015at 4270.448 kb on + strand, within BPHYT_RS19015at 4270.448 kb on + strand, within BPHYT_RS19015at 4270.448 kb on + strand, within BPHYT_RS19015at 4270.449 kb on - strand, within BPHYT_RS19015at 4270.449 kb on - strand, within BPHYT_RS19015at 4270.449 kb on - strand, within BPHYT_RS19015at 4270.463 kb on + strand, within BPHYT_RS19015at 4270.547 kb on - strand, within BPHYT_RS19015at 4270.771 kb on + strandat 4271.216 kb on - strand, within BPHYT_RS19020at 4271.224 kb on + strand, within BPHYT_RS19020at 4271.225 kb on - strand, within BPHYT_RS19020at 4271.225 kb on - strand, within BPHYT_RS19020at 4271.321 kb on + strand, within BPHYT_RS19020at 4271.321 kb on + strand, within BPHYT_RS19020at 4271.321 kb on + strand, within BPHYT_RS19020at 4271.321 kb on + strand, within BPHYT_RS19020at 4271.321 kb on + strand, within BPHYT_RS19020at 4271.321 kb on + strand, within BPHYT_RS19020at 4271.322 kb on - strand, within BPHYT_RS19020at 4271.324 kb on - strand, within BPHYT_RS19020at 4271.325 kb on + strand, within BPHYT_RS19020at 4271.325 kb on + strand, within BPHYT_RS19020at 4271.325 kb on + strand, within BPHYT_RS19020at 4271.326 kb on - strand, within BPHYT_RS19020at 4271.444 kb on + strandat 4271.444 kb on + strandat 4271.444 kb on + strandat 4271.445 kb on - strandat 4271.445 kb on - strandat 4271.446 kb on + strandat 4271.446 kb on + strandat 4271.446 kb on + strandat 4271.447 kb on - strandat 4271.484 kb on + strandat 4271.493 kb on + strandat 4271.494 kb on - strandat 4271.601 kb on + strand, within BPHYT_RS19025at 4271.601 kb on + strand, within BPHYT_RS19025at 4271.602 kb on - strand, within BPHYT_RS19025

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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4,269,107 + BPHYT_RS19010 0.32 -5.3
4,269,108 - BPHYT_RS19010 0.32 -2.1
4,269,128 + BPHYT_RS19010 0.34 -2.6
4,269,218 + BPHYT_RS19010 0.43 -3.3
4,269,218 + BPHYT_RS19010 0.43 -3.3
4,269,218 + BPHYT_RS19010 0.43 -3.3
4,269,218 + BPHYT_RS19010 0.43 -5.2
4,269,608 + BPHYT_RS19010 0.82 -4.0
4,269,608 + BPHYT_RS19010 0.82 -4.2
4,269,702 - -0.7
4,269,812 + -4.1
4,270,022 + BPHYT_RS19015 0.25 -3.4
4,270,286 + BPHYT_RS19015 0.55 -2.6
4,270,286 + BPHYT_RS19015 0.55 -1.5
4,270,287 - BPHYT_RS19015 0.55 -4.3
4,270,287 - BPHYT_RS19015 0.55 -4.0
4,270,334 + BPHYT_RS19015 0.61 -3.7
4,270,424 + BPHYT_RS19015 0.71 -5.4
4,270,425 - BPHYT_RS19015 0.71 -4.1
4,270,425 - BPHYT_RS19015 0.71 -2.3
4,270,425 - BPHYT_RS19015 0.71 -1.0
4,270,425 - BPHYT_RS19015 0.71 -2.7
4,270,425 - BPHYT_RS19015 0.71 +1.9
4,270,446 + +1.3
4,270,448 + BPHYT_RS19015 0.74 -4.8
4,270,448 + BPHYT_RS19015 0.74 -4.4
4,270,448 + BPHYT_RS19015 0.74 -3.3
4,270,448 + BPHYT_RS19015 0.74 -3.3
4,270,448 + BPHYT_RS19015 0.74 -2.1
4,270,449 - BPHYT_RS19015 0.74 -4.2
4,270,449 - BPHYT_RS19015 0.74 -5.8
4,270,449 - BPHYT_RS19015 0.74 -4.8
4,270,463 + BPHYT_RS19015 0.76 -1.5
4,270,547 - BPHYT_RS19015 0.85 -2.6
4,270,771 + -1.4
4,271,216 - BPHYT_RS19020 0.58 +0.1
4,271,224 + BPHYT_RS19020 0.59 +0.3
4,271,225 - BPHYT_RS19020 0.59 -2.2
4,271,225 - BPHYT_RS19020 0.59 +1.3
4,271,321 + BPHYT_RS19020 0.76 +0.9
4,271,321 + BPHYT_RS19020 0.76 -1.0
4,271,321 + BPHYT_RS19020 0.76 -0.6
4,271,321 + BPHYT_RS19020 0.76 -0.2
4,271,321 + BPHYT_RS19020 0.76 -1.0
4,271,321 + BPHYT_RS19020 0.76 -0.3
4,271,322 - BPHYT_RS19020 0.76 -1.1
4,271,324 - BPHYT_RS19020 0.77 -1.1
4,271,325 + BPHYT_RS19020 0.77 -0.9
4,271,325 + BPHYT_RS19020 0.77 -0.7
4,271,325 + BPHYT_RS19020 0.77 +0.5
4,271,326 - BPHYT_RS19020 0.77 -0.4
4,271,444 + -2.3
4,271,444 + +0.6
4,271,444 + -1.4
4,271,445 - -0.1
4,271,445 - +0.1
4,271,446 + -0.7
4,271,446 + -0.1
4,271,446 + -3.8
4,271,447 - -1.4
4,271,484 + -0.8
4,271,493 + -0.9
4,271,494 - -0.3
4,271,601 + BPHYT_RS19025 0.30 -0.9
4,271,601 + BPHYT_RS19025 0.30 -0.7
4,271,602 - BPHYT_RS19025 0.30 +0.2

Or see this region's nucleotide sequence