Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS17410

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS17400 and BPHYT_RS17405 are separated by 30 nucleotidesBPHYT_RS17405 and BPHYT_RS17410 are separated by 46 nucleotidesBPHYT_RS17410 and BPHYT_RS17415 are separated by 84 nucleotides BPHYT_RS17400: BPHYT_RS17400 - anthranilate phosphoribosyltransferase, at 3,942,611 to 3,943,642 _RS17400 BPHYT_RS17405: BPHYT_RS17405 - indole-3-glycerol phosphate synthase, at 3,943,673 to 3,944,458 _RS17405 BPHYT_RS17410: BPHYT_RS17410 - adenylate cyclase, at 3,944,505 to 3,945,131 _RS17410 BPHYT_RS17415: BPHYT_RS17415 - uracil-DNA glycosylase, at 3,945,216 to 3,946,061 _RS17415 Position (kb) 3944 3945 3946Strain fitness (log2 ratio) -1 0 1 2 3at 3943.517 kb on + strand, within BPHYT_RS17400at 3943.518 kb on - strand, within BPHYT_RS17400at 3943.518 kb on - strand, within BPHYT_RS17400at 3943.648 kb on + strandat 3943.661 kb on - strandat 3943.672 kb on + strandat 3943.672 kb on + strandat 3943.672 kb on + strandat 3943.672 kb on + strandat 3943.672 kb on + strandat 3943.673 kb on - strandat 3943.673 kb on - strandat 3943.673 kb on - strandat 3943.673 kb on - strandat 3943.673 kb on - strandat 3943.694 kb on - strandat 3943.985 kb on + strand, within BPHYT_RS17405at 3944.492 kb on - strandat 3944.492 kb on - strandat 3944.618 kb on + strand, within BPHYT_RS17410at 3944.622 kb on + strand, within BPHYT_RS17410at 3944.623 kb on - strand, within BPHYT_RS17410at 3945.067 kb on + strand, within BPHYT_RS17410at 3945.067 kb on + strand, within BPHYT_RS17410at 3945.112 kb on - strandat 3946.059 kb on + strandat 3946.059 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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3,943,517 + BPHYT_RS17400 0.88 +0.5
3,943,518 - BPHYT_RS17400 0.88 -0.2
3,943,518 - BPHYT_RS17400 0.88 -0.0
3,943,648 + +0.8
3,943,661 - -0.3
3,943,672 + +0.5
3,943,672 + +0.1
3,943,672 + +2.9
3,943,672 + -0.2
3,943,672 + -0.5
3,943,673 - -1.3
3,943,673 - +0.3
3,943,673 - -0.1
3,943,673 - +0.9
3,943,673 - +0.3
3,943,694 - +0.5
3,943,985 + BPHYT_RS17405 0.40 -0.3
3,944,492 - -1.3
3,944,492 - +2.2
3,944,618 + BPHYT_RS17410 0.18 +1.0
3,944,622 + BPHYT_RS17410 0.19 +1.6
3,944,623 - BPHYT_RS17410 0.19 -0.2
3,945,067 + BPHYT_RS17410 0.90 +0.7
3,945,067 + BPHYT_RS17410 0.90 -0.2
3,945,112 - +0.8
3,946,059 + -1.3
3,946,059 + -1.2

Or see this region's nucleotide sequence