Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS15395 and BPHYT_RS15400 overlap by 4 nucleotides BPHYT_RS15400 and BPHYT_RS15405 are separated by 149 nucleotides BPHYT_RS15405 and BPHYT_RS15410 are separated by 106 nucleotides
BPHYT_RS15395: BPHYT_RS15395 - 6,7-dimethyl-8-ribityllumazine synthase, at 3,494,922 to 3,495,428
_RS15395
BPHYT_RS15400: BPHYT_RS15400 - N utilization substance protein B homolog, at 3,495,425 to 3,495,859
_RS15400
BPHYT_RS15405: BPHYT_RS15405 - aminotransferase, at 3,496,009 to 3,497,205
_RS15405
BPHYT_RS15410: BPHYT_RS15410 - hypothetical protein, at 3,497,312 to 3,498,493
_RS15410
Position (kb)
3496
3497
3498 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3495.474 kb on - strand, within BPHYT_RS15400 at 3495.956 kb on + strand at 3495.956 kb on + strand at 3495.957 kb on - strand at 3495.957 kb on - strand at 3496.003 kb on - strand at 3496.008 kb on + strand at 3496.072 kb on + strand at 3496.073 kb on - strand at 3496.073 kb on - strand at 3496.134 kb on + strand, within BPHYT_RS15405 at 3496.134 kb on + strand, within BPHYT_RS15405 at 3496.135 kb on - strand, within BPHYT_RS15405 at 3496.135 kb on - strand, within BPHYT_RS15405 at 3496.219 kb on + strand, within BPHYT_RS15405 at 3496.220 kb on - strand, within BPHYT_RS15405 at 3496.270 kb on + strand, within BPHYT_RS15405 at 3496.271 kb on - strand, within BPHYT_RS15405 at 3496.282 kb on + strand, within BPHYT_RS15405 at 3496.282 kb on + strand, within BPHYT_RS15405 at 3496.282 kb on + strand, within BPHYT_RS15405 at 3496.282 kb on + strand, within BPHYT_RS15405 at 3496.282 kb on + strand, within BPHYT_RS15405 at 3496.402 kb on + strand, within BPHYT_RS15405 at 3496.403 kb on - strand, within BPHYT_RS15405 at 3496.458 kb on + strand, within BPHYT_RS15405 at 3496.626 kb on + strand, within BPHYT_RS15405 at 3496.645 kb on + strand, within BPHYT_RS15405 at 3496.645 kb on + strand, within BPHYT_RS15405 at 3496.645 kb on + strand, within BPHYT_RS15405 at 3496.646 kb on - strand, within BPHYT_RS15405 at 3496.646 kb on - strand, within BPHYT_RS15405 at 3496.729 kb on + strand, within BPHYT_RS15405 at 3496.987 kb on + strand, within BPHYT_RS15405 at 3496.987 kb on + strand, within BPHYT_RS15405 at 3496.988 kb on - strand, within BPHYT_RS15405 at 3496.988 kb on - strand, within BPHYT_RS15405 at 3497.046 kb on + strand, within BPHYT_RS15405 at 3497.122 kb on + strand at 3497.122 kb on + strand at 3497.122 kb on + strand at 3497.619 kb on + strand, within BPHYT_RS15410 at 3497.620 kb on - strand, within BPHYT_RS15410 at 3497.734 kb on - strand, within BPHYT_RS15410 at 3497.806 kb on - strand, within BPHYT_RS15410
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 3,495,474 - BPHYT_RS15400 0.11 +0.9 3,495,956 + -1.3 3,495,956 + +1.8 3,495,957 - -0.1 3,495,957 - +0.6 3,496,003 - +0.0 3,496,008 + -0.5 3,496,072 + +1.7 3,496,073 - +0.0 3,496,073 - -2.8 3,496,134 + BPHYT_RS15405 0.10 +0.1 3,496,134 + BPHYT_RS15405 0.10 +0.2 3,496,135 - BPHYT_RS15405 0.11 -0.7 3,496,135 - BPHYT_RS15405 0.11 +0.4 3,496,219 + BPHYT_RS15405 0.18 +0.2 3,496,220 - BPHYT_RS15405 0.18 -0.0 3,496,270 + BPHYT_RS15405 0.22 +0.8 3,496,271 - BPHYT_RS15405 0.22 +0.4 3,496,282 + BPHYT_RS15405 0.23 +0.5 3,496,282 + BPHYT_RS15405 0.23 +1.4 3,496,282 + BPHYT_RS15405 0.23 +0.2 3,496,282 + BPHYT_RS15405 0.23 -1.3 3,496,282 + BPHYT_RS15405 0.23 -0.1 3,496,402 + BPHYT_RS15405 0.33 -0.3 3,496,403 - BPHYT_RS15405 0.33 +0.4 3,496,458 + BPHYT_RS15405 0.38 -0.1 3,496,626 + BPHYT_RS15405 0.52 -0.1 3,496,645 + BPHYT_RS15405 0.53 +0.3 3,496,645 + BPHYT_RS15405 0.53 +0.3 3,496,645 + BPHYT_RS15405 0.53 -1.0 3,496,646 - BPHYT_RS15405 0.53 -2.5 3,496,646 - BPHYT_RS15405 0.53 +0.3 3,496,729 + BPHYT_RS15405 0.60 +0.9 3,496,987 + BPHYT_RS15405 0.82 -0.5 3,496,987 + BPHYT_RS15405 0.82 +0.9 3,496,988 - BPHYT_RS15405 0.82 +0.4 3,496,988 - BPHYT_RS15405 0.82 +0.4 3,497,046 + BPHYT_RS15405 0.87 +0.2 3,497,122 + +0.4 3,497,122 + +1.2 3,497,122 + -0.6 3,497,619 + BPHYT_RS15410 0.26 -0.1 3,497,620 - BPHYT_RS15410 0.26 +1.2 3,497,734 - BPHYT_RS15410 0.36 -0.3 3,497,806 - BPHYT_RS15410 0.42 +1.0
Or see this region's nucleotide sequence