Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS15400

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS15390 and BPHYT_RS15395 are separated by 49 nucleotidesBPHYT_RS15395 and BPHYT_RS15400 overlap by 4 nucleotidesBPHYT_RS15400 and BPHYT_RS15405 are separated by 149 nucleotides BPHYT_RS15390: BPHYT_RS15390 - 3,4-Dihydroxy-2-butanone 4-phosphate synthase, at 3,493,736 to 3,494,872 _RS15390 BPHYT_RS15395: BPHYT_RS15395 - 6,7-dimethyl-8-ribityllumazine synthase, at 3,494,922 to 3,495,428 _RS15395 BPHYT_RS15400: BPHYT_RS15400 - N utilization substance protein B homolog, at 3,495,425 to 3,495,859 _RS15400 BPHYT_RS15405: BPHYT_RS15405 - aminotransferase, at 3,496,009 to 3,497,205 _RS15405 Position (kb) 3495 3496Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3494.714 kb on + strand, within BPHYT_RS15390at 3494.714 kb on + strand, within BPHYT_RS15390at 3494.736 kb on + strand, within BPHYT_RS15390at 3494.736 kb on + strand, within BPHYT_RS15390at 3495.474 kb on - strand, within BPHYT_RS15400at 3495.956 kb on + strandat 3495.956 kb on + strandat 3495.957 kb on - strandat 3495.957 kb on - strandat 3496.003 kb on - strandat 3496.008 kb on + strandat 3496.072 kb on + strandat 3496.073 kb on - strandat 3496.073 kb on - strandat 3496.134 kb on + strand, within BPHYT_RS15405at 3496.134 kb on + strand, within BPHYT_RS15405at 3496.135 kb on - strand, within BPHYT_RS15405at 3496.135 kb on - strand, within BPHYT_RS15405at 3496.219 kb on + strand, within BPHYT_RS15405at 3496.220 kb on - strand, within BPHYT_RS15405at 3496.270 kb on + strand, within BPHYT_RS15405at 3496.271 kb on - strand, within BPHYT_RS15405at 3496.282 kb on + strand, within BPHYT_RS15405at 3496.282 kb on + strand, within BPHYT_RS15405at 3496.282 kb on + strand, within BPHYT_RS15405at 3496.282 kb on + strand, within BPHYT_RS15405at 3496.282 kb on + strand, within BPHYT_RS15405at 3496.402 kb on + strand, within BPHYT_RS15405at 3496.403 kb on - strand, within BPHYT_RS15405at 3496.458 kb on + strand, within BPHYT_RS15405at 3496.626 kb on + strand, within BPHYT_RS15405at 3496.645 kb on + strand, within BPHYT_RS15405at 3496.645 kb on + strand, within BPHYT_RS15405at 3496.645 kb on + strand, within BPHYT_RS15405at 3496.646 kb on - strand, within BPHYT_RS15405at 3496.646 kb on - strand, within BPHYT_RS15405at 3496.729 kb on + strand, within BPHYT_RS15405

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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3,494,714 + BPHYT_RS15390 0.86 -0.3
3,494,714 + BPHYT_RS15390 0.86 -1.8
3,494,736 + BPHYT_RS15390 0.88 -1.1
3,494,736 + BPHYT_RS15390 0.88 -0.3
3,495,474 - BPHYT_RS15400 0.11 +0.9
3,495,956 + -1.3
3,495,956 + +1.8
3,495,957 - -0.1
3,495,957 - +0.6
3,496,003 - +0.0
3,496,008 + -0.5
3,496,072 + +1.7
3,496,073 - +0.0
3,496,073 - -2.8
3,496,134 + BPHYT_RS15405 0.10 +0.1
3,496,134 + BPHYT_RS15405 0.10 +0.2
3,496,135 - BPHYT_RS15405 0.11 -0.7
3,496,135 - BPHYT_RS15405 0.11 +0.4
3,496,219 + BPHYT_RS15405 0.18 +0.2
3,496,220 - BPHYT_RS15405 0.18 -0.0
3,496,270 + BPHYT_RS15405 0.22 +0.8
3,496,271 - BPHYT_RS15405 0.22 +0.4
3,496,282 + BPHYT_RS15405 0.23 +0.5
3,496,282 + BPHYT_RS15405 0.23 +1.4
3,496,282 + BPHYT_RS15405 0.23 +0.2
3,496,282 + BPHYT_RS15405 0.23 -1.3
3,496,282 + BPHYT_RS15405 0.23 -0.1
3,496,402 + BPHYT_RS15405 0.33 -0.3
3,496,403 - BPHYT_RS15405 0.33 +0.4
3,496,458 + BPHYT_RS15405 0.38 -0.1
3,496,626 + BPHYT_RS15405 0.52 -0.1
3,496,645 + BPHYT_RS15405 0.53 +0.3
3,496,645 + BPHYT_RS15405 0.53 +0.3
3,496,645 + BPHYT_RS15405 0.53 -1.0
3,496,646 - BPHYT_RS15405 0.53 -2.5
3,496,646 - BPHYT_RS15405 0.53 +0.3
3,496,729 + BPHYT_RS15405 0.60 +0.9

Or see this region's nucleotide sequence